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Green synthesis of Copper Nanoparticles from Coleus amboinicus stem extract and its Characterization, Antibacterial potential study against Staphylococcus aureus

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Author Affiliations

  • 1Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India
  • 2Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India
  • 3Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India
  • 4Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India
  • 5Insearch Laboratory, Bengaluru, Karnataka, India

Res. J. Recent Sci., Volume 15, Issue (3), Pages 29-35, July,2 (2026)

Abstract

The green synthesis of nanoparticles is widely preferred due to their versatile nature, and this approach enabled the successful synthesis of nanoparticles in the laboratory. The plant extract from Coleus amboinicus possesses antibacterial properties, and copper, a natural antimicrobial element, has been used since ancient times. Combining them as nanoparticles enhanced their antibacterial activity, effectively inhibiting the growth of Staphylococcus aureus, which was confirmed by agar well diffusion, showing the largest zone of inhibition at the maximum dose of 40 μl, and nutrient broth assay, exhibiting 88% inhibition at the same dose. Biofilm disruption was demonstrated using the Congo red agar method, linked to reactive oxygen species generation. 3.8% SOD activity was shown by Superoxide dismutase assay, indicating oxidative stress and confirming ROS presence. These findings validate the antibacterial potential of Mexican mint–copper nanoparticles, highlighting their applicability in biomedical and environmental disciplines.

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