International Science Community Association Research Journal of Recent Sciences 15 3 2026 07 2 Green synthesis of Copper Nanoparticles from Coleus amboinicus stem extract and its Characterization, Antibacterial potential study against Staphylococcus aureus 29 35 EN Sahu Suresh 1 Government Engineering College, Ajmer, Rajasthan, India Kumar Vinod 1 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Aggarwal Akanksha 2 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Kulshershtha Amit 3 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Kumar Manish 4 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Kumar Shanta 5 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Tripathi Raj Kumar 6 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Saini Yashwant Kumar 7 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Gupta Shyam Manohar 8 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Ram Anshu Beulah 1 Department of Biotechnology, Nrupathunga University, Nrupathunga Road, Bangalore, Karnataka 560001, India Kour Gurshish 2 Clinical Microbiology Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu 180 001, India P. Priya 1 Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India P.R. Druthi 2 Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India M. Jeevitha 3 Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India Kabhade Sarina P. 4 Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India Stella Bhupanapadu Sunkesula Mary 5 Insearch Laboratory, Bengaluru, Karnataka, India > Research Paper 2026 4 12 2026 07 2 The green synthesis of nanoparticles is widely preferred due to their versatile nature, and this approach enabled the successful synthesis of nanoparticles in the laboratory. The plant extract from Coleus amboinicus possesses antibacterial properties, and copper, a natural antimicrobial element, has been used since ancient times. Combining them as nanoparticles enhanced their antibacterial activity, effectively inhibiting the growth of Staphylococcus aureus, which was confirmed by agar well diffusion, showing the largest zone of inhibition at the maximum dose of 40 μl, and nutrient broth assay, exhibiting 88% inhibition at the same dose. Biofilm disruption was demonstrated using the Congo red agar method, linked to reactive oxygen species generation. 3.8% SOD activity was shown by Superoxide dismutase assay, indicating oxidative stress and confirming ROS presence. These findings validate the antibacterial potential of Mexican mint–copper nanoparticles, highlighting their applicability in biomedical and environmental disciplines. Copyright@ International Science Community Association