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Enhanced production and Purification of L-asparaginase from fusarium proliferatum and its biochemical characterization

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Author Affiliations

  • 1Plant Pathology and Microbiology Division, Regional Plant Resource Centre, Bhubaneswar-751015, Odisha, India
  • 2Plant Pathology and Microbiology Division, Regional Plant Resource Centre, Bhubaneswar-751015, Odisha, India
  • 3Plant Pathology and Microbiology Division, Regional Plant Resource Centre, Bhubaneswar-751015, Odisha, India

Res. J. Agriculture & Forestry Sci., Volume 13, Issue (2), Pages 1-11, April,8 (2025)

Abstract

Asparaginase converts L-asparagine to L-aspartic acid and has received considerable attention in the recent years for its anti-carcinogenic potential. L-asparaginase is a drug that is used to treat lymphoblastic cancer. The ability of a new strain to produce extracellular L-asparaginase was investigated in this work. The study examined the production of L-asparaginase utilizing MCDB, which had the highest L-asparaginase activity. The optimization, synthesis, purification, and characterization of the L-Asparaginase enzyme obtained from Fusarium proliferatum are the main objectives of this investigation. After the crude enzyme was removed, it was precipitated with ammonium sulphate, filtered using a Sephadex column, and then further purified using ion exchange chromatography to yield L-asparaginase. To increase L-asparaginase synthesis, several sources and quantities of nutritional factors—such as carbon, nitrogen, and amino acid inducers—as well as physical parameters—such as pH and temperature—were adapted. The study findings indicated that the most favourable conditions for enzyme production were a pH of 8.0 at 37°C. The carbon and nitrogen sources that demonstrated the highest efficacy were Lactose (0.5%) and L-asparagine (0.5%), respectively. The yield was also high in vitamin-B1 (1%), sodium Nitrate (1%), Tween-20 (1%), Aspartic acid (1%), and Ammonium flouride (1%). The production of L-asparaginase on a large scale can be achieved through continuous fermentation using the medium composition outlined in the study.

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