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Impact of temperature and pH Variation on in-vitro Protocorm formation of Vanda tessellata (Roxb.) Hook. ex G.Don an endangered medicinal Orchid

Author Affiliations

  • 1 Department of Botany, Sarojini Naidu Govt. Girls Post Graduate (Autonomus) College Shivaji Nagar, Bhopal, MP, INDIA

Res. J. Recent Sci., Volume 4, Issue (IYSC-2015), Pages 10-13, September,2 (2015)


The present research work is carried out to study the In-vitro protocorm formation of an endangered medicinal orchid Vanda tessellata at different temperature(15C, 20C, 25C, 30C) and pH (4.5, 5.5, 6.5,7.5) . Immature seeds obtained from green pods successfully shown protocorm formation on basal media MS without various combinations of growth hormones. Highest protocorm formation (940.17%) were observed at temperature 20C and pH 5.5 , whereas the medium which is adjusted at temperature 15C, 25C, 30C and pH 4.5, 6.5, 7.5 showed poor protocorm formation.


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