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Binding studies of Ru(II) complex with DNA isolated from orange pulp extract

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Author Affiliations

  • 1Department of Chemistry and Research Centre, Scott Christian College (Autonomous) Nagercoil (Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli, Tamilnadu, India
  • 2Department of Chemistry and Research Centre, Scott Christian College (Autonomous) Nagercoil (Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli, Tamilnadu, India
  • 3Department of Chemistry and Research Centre, Scott Christian College (Autonomous) Nagercoil (Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli, Tamilnadu, India

Res. J. Recent Sci., Volume 14, Issue (3), Pages 14-18, July,2 (2025)

Abstract

For a long time, researchers have been closely examining how metal complexes interact with DNA in order to create novel compounds or medications for use in medicine. Ru (II) complexes have shown an excellent DNA binding results. These complexes bind to DNA through intercalative mode. The binding of Ru (II) phenanthroline complex [RuL3]2+ (where L=1, 10-phenanthroline) with DNA of guava fruit extract have been studied in aqueous medium by means of absorption and emission spectral techniques. Using the Benesi-Hildebrand equation, the complex's binding constant (Kb) with the DNA extracted from guava fruit was ascertained. The luminophore and the DNA molecule have a hydrophobic ground state interaction. It was found to be 9.506x104M-1. The ligands in the complex and the purity of the DNA determine the binding constant's value.

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