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Innovative Lignin Nanoparticles: Biocontrol Agents against Garlic Crop Pathogen Fungi

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Author Affiliations

  • 1Microbiology Laboratory, Dept. of Basic Sciences, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni-Solan (Himachal Pradesh), India and Department of Biological and Chemical Sciences, Baba Farid Colleges, Bathinda (Punjab), India
  • 2Microbiology Laboratory, Dept. of Basic Sciences, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni-Solan (Himachal Pradesh), India

Res. J. Recent Sci., Volume 13, Issue (3), Pages 9-19, July,2 (2024)

Abstract

By being employed for diverse applications by itself, lignin, which is thrown as waste during lignocellulosic biomass pre-treatments for the manufacture of biofuel, biogas, paper, and several other products, can greatly boost the economic viability of bio-refineries. We focused on discarded lignin and its usage in the synthesis of nanoparticles and their application as biocontrol agents for garlic crop. By fractionating agricultural waste biomass using the modified organosolv technique, lignin, which served as a capping agent for the synthesis of zinc oxide nanoparticles, was obtained and characterized using the Nuclear Magnetic Resonance (NMR) technique, whereas, the analytical investigations of lignin-derived zinc oxide nanoparticles (L-ZnO NPs) were carried out using the NMR, UV- Visible spectroscopy, Field Emission Scanning Electron Microscopy, and High Resolution Transmission Electron Microscopy techniques, which validated their production, morphology, shape, and size. The NPs were effective against 3 phyto-pathogenic fungi (Fusarium oxysporum, Fusarium proliferatum and Stemphylium vesicarium) that cause dry rot, basal rot, and blight of important cash crop- garlic (Allium sativum) in Himachal Pradesh, India. This was done in a lab setting before a net-house pot trial on garlic, where the L-ZnO NPs demonstrated strong antagonistic efficacy against the phyto-pathogenic fungi. Additionally, the environmentally friendly, cost-effective lignin-derivation from agricultural waste and subsequent lignin-mediated zinc oxide nanoparticle synthesis process would help in the production of numerous value-added materials in the future, as well as a potent antifungal agent for use in agriculture.

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