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Comparison of Microwave Irradiation and Conventional Synthesis of 2-thiobarbituric acid Derivatives and in vitro Evaluation of Antimicrobial and Cytotoxic activity

Author Affiliations

  • 1Department of Applied Chemistry and Chemical Technology, Islamic University, Kushtia-7003, Bangladesh
  • 2Department of Applied Chemistry and Chemical Technology, Islamic University, Kushtia-7003, Bangladesh
  • 3Department of Applied Chemistry and Chemical Technology, Islamic University, Kushtia-7003, Bangladesh and Jessore Science and Technology University, Jessore-7408, Bangladesh

Res.J.chem.sci., Volume 6, Issue (10), Pages 24-34, October,18 (2016)


The research work was involved in rapid and efficient procedure for the attachment of 2-thiobarbituric acid with arylidene acetophenone under microwave irradiation (MWI) and conventional heating. We found that the preparation time for the compounds was reduced from 24 hours to 5-10 minutes duing microwave heating. The yield also very poor (78-80%) for the compounds during conventional heating, but in MWI methods the yields were observed 96.68-98.50% which was comparatively too high. By using FT-IR, 1H-NMR spectral data, the synthesized compounds were characterized. The cytotoxic and antimicrobial activities of the compounds were also investigated. Staphylococcus aureus, Bacillus megaterium, Pseudomonas aeruginosa and Escherichia coli revealed the inhibition zone were 8-14 mm where sample concentration was 100 µg/disc. However, cytotoxic analysis, the mortality 49-89% were appeared when sample concentration were 0.78- 6.25(µg/ml) and more than 12.5 (µg/ml) concentration showed 100% mortality. The presence of a reactive and unsaturated ketone function in synthesized compounds may be responsible for their potential antimicrobial and cytotoxic activity.


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