α-Glucosidase inhibition, antioxidant and cytotoxicity activities of semi-ethanolic extracts of Bridellia ferruginea benth. and Ceiba pentandra L. Gaerth from Benin
Author Affiliations
- 1Laboratoire d'Etude et de Recherche en Chimie Appliquée, Ecole Polytechnique d'Abomey Calavi/Université d'Abomey Calavi, Cotonou, BENIN
- 2Laboratoire de chimie organique, UMR INSERM 990, Faculté de Pharmacie rue Montalembert, Clermont- Ferrand, FRANCE
- 3Herbier National, Département de Botanique, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, BENIN
Res.J.chem.sci., Volume 2, Issue (12), Pages 31-36, December,18 (2012)
Abstract
The use of plant extracts in the treatment of human disease requires a definition of optimal conditions. This study objective is to evaluate the inhibition capacity of -glucosidase activity of Bridelia ferruginea benth bark and Ceiba pentandra L. Gaerthroot semi-ethanolicextracts compared to acarbose. Their antioxidant activity were also tested by three techniques: 2,2-diphenyl-1-picrylhydrazyl (DPPH)radical scavenging, Ferric reducing antioxidant power (FRAP) and the Oxygen Radical Absorbance Capacity(ORAC). Results indicated that these extracts have antioxidant property and -Glucosidase inhibitory activity (IC50 = 1.4 ± 0.04 µg/ mL for B. ferruginea and 51 ± 0.7 µg / mL for C. pentandra) higher than the reference compound acarbose (IC50 = 726 ± 15 mg / mL). Bridelia ferruginea extract was the most active of the two. In vitro cytotoxicity evaluation of the extracts was done by fluorometric assay: Resazurin reduction test onhuman fibroblast primary culture have showed a very low toxicity. Bridelia ferruginea and Ceiba pentandra The semi-ethanolic extracts could therefore constitute a credible alternative to replace the expensive synthetic drugs in the treatment of diabetes.
References
- Shrabana C., Tuhin K. B., Tapan S., Begum R., Liaquat A., Khan A. K., Nilufer N., Mosihuzzaman M. and Biswapati M., Antidiabetic activity of Caesalpinia bonducella F. in chronic type 2 diabetic model in Long-Evans rats and evaluation of insulin secretagogue property of its fractions on isolated islets, J. Ethnopharmacol., 97,117-122 (2005)
- Abayomi S., Felicitas C. and Kurt H., Plantes médicinales et médecine traditionnelle d'Afrique, Académie Suisse des sciences naturelles Paris Karthala, 1, 378 (2010)
- Fontbonne A., Favier F. and Papoz L., Le diabète de type 2 dans le monde, analyse d’une épidémie, Flammarion(2003)
- Salah Z., Christian B. and Kaoual M, Approche épidémiologique du diabète en milieux urbain et rural dans la région de Tlemcen (Ouest algérien), Cah. Etude Rech. Francoph./Santé,17(1),(2007)
- World Health Organization: Who launches the first global strategy on traditional medicine, Press release,38, Geneva (2002)
- Ambe G. A. and Malaisse F., Diversité des plantes médicinales et ethnotaxonomie en pays malinke de Côte d'Ivoire, Rev. Méd. Pharm.Afr.,14, 121-130 (2000)
- Akoègninou A., Van der Burg W. J. and Van der Maesen L. J. G., Flore analytique du Bénin Many line drawings, XXII, 1034 Hardcover (2006)
- Addae-Mensah I. and Munenge R.W., Quercetin-3-neohesperidoside (Ruti) and other flavonoids as the active hypoglycaemic agents of Bridelia Ferruginea, Fitoterapia, 4, 359-362 (1989)
- Onunkwo G. C., Akah P. A. and Udeala O. K., Studies on Bridelia ferruginea Leaves Stability and Hypoglycaemic Actions of the Leaf Extract Tablets, Phytother. Res.,10,418-420 (1996)
- Ladeji O., Omekarah I. and Solomon M., Hypoglycemic properties of aqueous bark extract of Ceiba pentandra in streptozotocin-induced diabetic rats, J. Ethnopharmacol.,84, 139-142 (2003)
- Dzeufiet P. D., Dieudonné Y. O., Léonard T., Emmanuel A. A., Théophile D., Sokeng D. S. and Pierre K., Antidiabetic Effect Of Ceiba Pentandra extract on StreptozoTocin-Induced Non-Insulin-Dependent Diabetic (Niddm), Afr. J. Tradit. Complement. Altern. Med.,4, 47-54 (2007)
- Kalu F. N., OguguaV. N., Ujowundu C.O. and Chinekeokwu C. R. K., Chemical Composition and Acute Toxicity Studies on the Aqueous Extract of Combretum dolichopentalum Leaf in Swiss Albino Mice, Res. J. Chem. Sci., 1(8), 72-75 (2011)
- Rattanachitthawat S., Suwannalert P., Riengrojpitak S., Chaiyasut C. and Pantuwatana S., Phenolic content and antioxidant activities in red unpolished Thai rice prevents oxidative stress in rats, J. Med. Plant Res.,4, 796-801 (2010)
- Djeridane A., Yousfi M., Nadjemi B., Boutassouna D., Stocker P. and Vidal N., Antioxidant activity of some Algerian medicinal plant extracts containing phenolic compounds, Food Chem.,97, 654-660 (2006)
- Khady B., Emmanuel T., Jacqueline D., Ndiaga C., Philippe T., Étude comparative des composés phénoliques, du pouvoir antioxydant de différentes variétés de sorgho sénégalais et des enzymes amylolytiques de leur malt, Biotechnol. Agron. Soc. Environ.,14, 131-139 (2010)
- Tiwari, A. K., Swapna, M., Ayesha, S. B., Zehra, A., Agawane, S. B. and Madhusudana, K, Identification of proglycemic and antihyperglycemic activity in antioxidant rich fraction of some common food grains International, Food Res. J., 18, 915-923 (2011)
- Djandé A., Kiendrébéogo M., Compaoré M., Kaboré L., Nacoulma G. O., Aycard J-P. and Saba A., Antioxidant Potentialities of 4-Acyl isochroman-1,3-Diones, Res. J Chem. Sci., 1(5), 88-90(2011)
- Piljac-egarac J., Stipevi T. and Belšak A., Antioxidant properties and phenolic content of different floral origin honeys, J. Apiprod. Apimed. Sci., 1, 43 – 50 (2009)
- Ou B., Maureen H.W., and Ronald L. P., Development and Validation of an Improved Oxygen Radical Absorbance Capacity Assay Using Fluorescein as the Fluorescent Probe, J. Agric. Food Chem.49, 4619-4626 (2001)
- Mathieu S., Éric D., Bettina A., Michelle P. and Pascale M., Synthesis and antiproliferative activities of indolin-2-one derivatives bearing amino acid moieties, Eur. J. Med. Chem..,41, 709–716 (2006)
- Stuart A. R., Gulve E. A. and Wang, M., Chemistry and biochemistry of type 2 diabetes, Chem. Rev.104, 1255–1282 (2004)
- Youn J.Y., Park H.Y. and Cho K.H., Anti-hyperglycemic activity of Commelina communis L. inhibition of a-glucosidase, Diabetes Res. Clin.,66S, S149-S155 (2004)
- Shinde J.T., Taldone M., Barletta N., Kunaparaju B., Hu S., Kumar J.P., and Zito S.W., -Glucosidase inhibitory activity of Syzygium cumini (Linn.) Skeels seed kernel in vitro and in Goto-Kakizaki (GK) rats, Carbohydr. Res.,343, 1278-1281 (2008)
- Liu R. H., and Finley J., Potential cell culture models for antioxidant research, J. Agric. Food. Chem., 53, 4311–4314 (2005)
- Hsieh P-C., Guan-Jhong H., Yu-Ling H.O., Yaw-Huei L., Shyh-Shyun H., Ying-Chen C., Mu-Chuan T. and Yuan-Shiun C., Activities of antioxidants, -Glucosidase inhibitors and aldose reductase inhibitors of the aqueous extracts of four Flemingia species in Taiwan, Bot. Stud., 51, 293-302 (2010)
- Jung M., Park M., Chul H.L., Kang Y., Seok-Kang E. and Ki-Kim S., Antidiabetic agents from medicinal plants, Curr. Med. Chem.,13, 1–16 (2006)
- Apostolidis E., Young-In K. and Kalidas S., Potential of cranberry-based herbal synergies for diabetes and hypertension management, Asia Pac. J. Clin. Nutr., 15,433-441(2006)
- Kim M. H., Sung-Hoon J., Hae-Dong J., Mee S. L. and Young-In K., Antioxidant activity and -glucosidase inhibitory potential of onion (Allium cepa L.) extracts, Food Sci. Biotechnol., 19, 159-164 (2010)
- Huang D., Boxin O., and Ronald L. P., The Chemistry behind Antioxidant Capacity Assays, J. Agric. Food Chem.,53, 1841–1856 (2005)
- Sadhu S. K., Okuyama E., Fujumoto H. and Ishibashi M., Seperartion of leucas, aspara amedicinal plant of Bangladesh, guided by prostaglandin inhibitory and antioxidant activities, Chem. Pharm. Bull., 51, 595-598 (2003)
- Benzie I.F.F. and Szeto Y.T., Total Antioxidant Capacity of Teas by the Ferric Reducing / Antioxidant Power Assay, J. Agric. Food Chem.,47, 633-636 (1999)
- Jokhadze M., Eristavi L., Kutchukhidze J., Chariot A., Angenot L., Tits M., Jansen O., and Frédérich M., In vitro cytotoxicity of some medicinal plants from Georgian Amaryllidaceae, Phytother. Res.,21(7), 622-624 (2007)
- Rashid M. A., Gustafson K. R., Cardellina J.H. and Boyd M. R., A New Podophyllotoxin Derivative from Bridelia ferruginea, Nat. Prod. Lett.,14, 285-292 (2000)
- Castro M.A., Miguel del Corral J.M., Gordaliza M., Gómez-Zurita M.A., García P.A. and San Feliciano A., Chemoinduction of cytotoxic selectivity in Podophyllotoxin-related lignans, Phytochem. Rev.,2, 219–233 (2003)