International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Effects of Cu and Zn Supplementation on Metal Uptake by Hibiscus sabdariffa

Author Affiliations

  • 1Aix-Marseille Université, CNRS, LCE, FRE 3416, 13331 Marseille, FRANCE
  • 2Laboratoire pluridisciplinaire des sciences, Ecole Normale Supérieure, B.P 17009 Libreville, GABON

Res.J.chem.sci., Volume 2, Issue (11), Pages 45-50, November,18 (2012)


Pot experiment was carried out in order to assess the addition effects of copper (Cu) and zinc (Zn) on their distribution and those of other metals in plant tissues. The experiment consisted of growth Hibiscus sabdariffa, a leafy vegetable, for 30 days in a soil to which copper and zinc were added alone and in combination. At the end of the experiment roots, stems and leaves of plants were analyzed for metal uptake in tissues. The results show that the Cu and Zn contents in the plant tissues varied with their single and combined additions in soil. The Cu and Zn accumulation in plant tissues was as follows roots > stems > Leaves. An antagonist or synergic effect observed in root and stem tissues according to Cu or Zn was added in soil showed that Zn and Cu uptake in plant seemed to be controlled by the concentration of both metals in soil. Generally the Cu and Zn addition in soil had antagonistic effects with Cd, Cr, Fe, Mn, Ni and Pb in root and stem tissues. In opposite, the synergic effect observed with Cr, Fe and Mn uptake in leave tissues led to the conclusion that Cu and Zn can help to have metal deficiency decrease in chain food.


  1. World Health Organization Food and Agriculture Organization (WHO/FAO), Guidelines on Food Fortification with Micronutrients. Geneva WHO (2006)
  2. Bhattacharya T., Chakraborty S., Fadadu B. and Bhattacharya P., Heavy metal concentrations in Street and Leaf Deposited Dust in Anand city, India, Res. J. Chem. Sci., 1(5), 61-66 (2011)
  3. Nwajei G.E., Okwagi P., Nwajei R.I. and Obi-Iyeke G.E., Tomato Leaves and Fruits in the Vicinity of Paint Industry, Nigeria, Res. J. Recent Sci.,1(4), 22-26 (2012)
  4. Vaishnav V., Daga K., Chandra S. and Lal M., Adsorption Studies of Zn (II) ions from Wastewater using Calotropis procera as an Adsorbent, Res. J. Recent Sci., 1(ISC-2011), 160-165 (2012)
  5. Ogbe A.O. and George G.A.L., Nutritional and Anti-nutrient Composition of Melon Husks: Potential as Feed Ingredient in Poultry Diet, Res. J. Chem. Sci., 2(2), 35-39 (2012)
  6. Shrivastava S. and Dwivedi S., Effect of fly Ash Pollution on Fish Scales, Res. J. Chem. Sci., 1(9), 24-28 (2011)
  7. Mane T.T. and Raskar Smita S., Management of Agriculture Waste from Market Yard Through Vermicomposting, Res. J. Recent Sci., 1(ISC-2011) 289-296 (2012)
  8. Ayodele J.T. and Mohammed S.S., Zinc Speciation in Maize and Soils, Res. J. Chem. Sci., 1(4), 98-108 (2011)
  9. Abii T.A., Levels of Heavy Metals (Cr, Pb, Cd) Available for Plants within Abandoned Mechanic Workshops in Umuahia Metropolis, Res. J. Chem. Sci., 2(2), 79-82 (2012)
  10. Gaetke L.M. and Chow C.K., Copper Toxicity, Oxidative Stress, and Antioxidant Nutrients, Toxicol.189(1-2), 147-163 (2003)
  11. Turnlund J.R., Human Whole-Body Copper Metabolism, Am. J. Clin. Nutr., 67(5), 960-964 (1998)
  12. Uriu-Adams J.Y. and Keen C.L., Copper, Oxidative Stress, and Human Health, Mol. Aspects Med., 26(4-5), 268-298 (2005)
  13. Turley E., McKeown A., Bonham M. P., O’Connor J.M., Chopra M., Harvey L.J., Majsak-Newman G., Fairweather-Tait S.J., Bügel S., Sandström B., Rock E., Mazur A., Rayssiguier Y. and Strain J.J., Copper Supplementation in Humans does not affect the Susceptibility of Low Density Lipoprotein to in Vitro Induced Oxidation (FOODCUE project), Free Radical Biol. Med., 29(11), 1129-1134 (2000)
  14. Kaji M. and Nishi Y., Growth and Minerals: Zinc. Growth Genet. Horm.,22(1), 1-7 (2006)
  15. Jalbani N., Ahmed F., Kazi T.G., Rashid U., Munshi A.B. and Kandhro A., Determination of Essential Elements (Cu, Fe and Zn) in Juices of Commercially Available in Pakistan, Food Chem. Toxicol.,48(10), 2737-2740 (2010)
  16. Onianwa P.C., Adetola I.G., Iwegbue C.M.A., Ojo M.F. and Tella O.O., Trace Heavy Metals Composition of some Nigerian Beverages and Food Drinks, Food Chem., 66(3), 275–279 (1999)
  17. Abdulla M. and Suck C., Blood Levels of Copper, Iron, Zinc, and Lead in Adults in India and Pakistan and the Effect of Oral Zinc Supplementation for Six Weeks, Biol. Trace Elem. Res., 61(3), 323-331 (1998)
  18. Graham R.D., Micronutrient Deficiencies in Crops and their Global Significance, In: Alloway BG (ed.), Micronutrient Deficiencies in Global Crop Production, Springer, Dordrecht, 41-61 (2008)
  19. Nardi E.P., Evangelist E.S., Tormen L., Saint´Pierre T.D., Curtius A.J., de Souza S.S., Barbosa Jr F., The Use of Inductively Coupled Plasma Mass Spectrometry (ICP-MS) for the Determination of Toxic and Essential Elements in Different Types of Food Samples, Food Chem.,112(3), 727-732 (2009)
  20. Kabata-Pendias A., Trace Elements in Soils and Plants. Taylor and Francis Group, New York 548 (2011)
  21. Graham R.D., Absorption of Copper by Plant Roots, In: Copper in Soils and Plants. Loneragan J.F., Robson A.D., Graham R.D. (eds), Academic Press, New-York, 141-163 (1981)
  22. Shi J., Yuan X., Chen X., Wu B., Huang Y. and Chen Y., Copper Uptake and Its Effect on Metal Distribution in Root Growth Zones of Commelina communis Revealed by SRXRF, Biol. Trace Elem. Res., 141(1-3), 294-304 (2011)
  23. Aref F., Influence of Zinc and Boron Nutrition on Copper, Manganese and Iron Concentrations in Maize Leaf, Aust. J. Basic Appl. Sci.,5(7), 52-62 (2011)
  24. Zayed A., Lytle C.M., Qian J.H. and Terry N., Chromium Accumulation, Translocation and Chemical Speciation in Vegetable Crops, Planta,206(2), 293-299 (1998)
  25. World Health Organization (WHO), Nutrition. Micronutrient Deficiencies, Geneva, WHO (2011)
  26. van der Waals J.H. and Laker M.C., Micronutrient Deficiencies in Crops in Africa with Emphasis on Southern Africa. In: Alloway BJ (ed.) Micronutrient Deficiencies in Global Crop Production. Springer, Dordrecht 201-224 (2008)
  27. Holloway R.E., Graham R.D. and Stacey S.P., Micronutrient Deficiencies in Australian Field Crops, In: Alloway B.J. (ed.) Micronutrient Deficiencies in Global Crop Production, Springer, Dordrecht 63-92 (2008)
  28. Watts D.L., The Nutritional Relationships of Manganese, J. Orthomolec. Med., 5(4), 219-222 (1990)