Effect of Dietary Vitamin A Supplement on Serum Protein of Rats Infected with Trypanosoma brucei
Author Affiliations
- 1Nnamdi Azikiwe University Awka, Anambra State, NIGERIA
- 2 Federal University of Science and Technology Owerri, Imo State, NIGERIA
Res.J.chem.sci., Volume 2, Issue (11), Pages 61-63, November,18 (2012)
Abstract
The role of dietary vitamin A supplement on the serum protein of trypanosome-infected rats was studied. The rats were inoculated with trypanosomes intraperitoneally and samples were collected on fourth, eight, twelfth and sixteenth days of post infection. The experiment was carried out at the Department of Biochemistry, Nnamdi Azikiwe University Awka. 60 parasite free-albino rats were used, which were divided into four groups. Group A (control) was left uninfected with trypanosomes, group B and C were infected with trypanosomes and treated with 50mls and 100mls of vitamin A per kg of feed respectively and group D was infected and left untreated with vitamin A. Analyses of the sera using Bradford method and cellulose acetate electrophoresis showed that vitamin A influenced the state of hypoproteinaemia in the trypanosome-infected rats. This was manifested by a positive increase in the level of total serum protein concentration, albumin and beta-globulin. Vitamin A also delayed the proliferation of the parasites associated with trypanosomiasis.
References
- Morrison W.I., Murray M. and McIntyre W.I.M., Bovine trypanosomiasis, In Risstic M. and McIntyre, W.I.M. (Eds), Disease of Cattle in Tropics, The Hagne, Martinis Nijhoff Publishers, 469–497 (1981)
- Jackson G.J., Trypanosoma congolense: inheritance of susceptibility to infection in inbred strains of mice, Experimental Parasitology, 48, 378-383 (1979)
- Krampitz H.E., Beobach, tungen an experimentallan infection ostafrickascher Zebundider Mit wild Stamen Von, T. congolense, Trop. Para., 21, 1-30 (1970)
- Katungka-Rwakishaya E., Interaction between animal nutrition and parasites, studies with experimental trypanosomiasis in sheep, Pages 1–9, In (Lebbie, S.H. B. and Kagwini, E., Eds), Small Ruminant Research and Development in Africa, International Livestock Research Institute (ILRI) Nairobi Kenya (1996)
- Agyemang K., Dwinger R.H., Touray B.N., Jeannin P., Fofana D. and Grieve A.S., Effects of Nutrition on degree of aneamia and live weight changes in N’Dama cattle infected with trypanosomes, Livestock Production Science,26, 39–51 (1990)
- Little D.A., Dwinger R.H., Clifford D.J., Grieve A.S., Kora S. and Bojang M., Effect of Nutritional level and body condition on susceptibility of N’Dama cattle to T. congolense infection in the Gambia, Proceedings of the Nutrition society, 49, 209A (1990)
- Raza A., Khan F.K., Saced M.A. and Basir N.I., Effect of vitamin A on Growth Traits, Immuno-regulatory Organs and Immune Response in Broiler Chiken, J. Applied Ani. Res., 12, 81–88 (1997)
- Sijtsma R.S., West F.C., Pombout M.W.J. and Van Der Zipp J.A., The interaction between vitamin A status and Newcastle Disease virus infections in chicken, J. Nutr., 119, 932–939 (1991)
- Johannsen A.K.B., Jensen S.K. and Jakobsen K., A note on vitamin A activity of B-Carotene in rats, Acta Agric. Scand. Sect. J. Ani. Sc., 48, 260-263 (1998)
- Bang F.B., Bang B.G. and Foard M., Acute Newcastle viral infection of the upper respiratory tract of the chicken: 2, Effect of diets deficient in vitamin A on the infection, J. Ani. Path., 78, 417–426 (1995)
- Benynen A.C., Sijtsma R.S., Kiepurski A.K., West C.E., Baumans V., Herck Van. H., Stafleu R.F. and Van Tintele G., Objective clinical examination of poultry as illustrated by the comparison of chicken with different vitamin A status, British J. An. Sc., 23, 307–312 (1989)
- Herbert W.J. and Lumbsden W.H.R., Trypanosoma brucei, A rapid matching method for estimating the host’s parasitemia, Expt. Para., 40, 427-431 (1976)
- Nauss K.M., Phua C.C., Ambrodi L. and Newberne P.M., Immunological changes during progressive stages of vitamin A deficiency in the rats, J. Nutr., 115909-918 (1985)