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Simple and sensitive LC-ESI-MS method for estimation of sildenafil in plasma samples

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Author Affiliations

  • 1Synergen Bio Private Limited, Old Mumbai - Pune Highway, Wakadewadi, Shivajinagar, Pune, Maharashtra - 411003, India
  • 2Synergen Bio Private Limited, Old Mumbai - Pune Highway, Wakadewadi, Shivajinagar, Pune, Maharashtra - 411003, India
  • 3Synergen Bio Private Limited, Old Mumbai - Pune Highway, Wakadewadi, Shivajinagar, Pune, Maharashtra - 411003, India

Res.J.chem.sci., Volume 15, Issue (1), Pages 1-7, February,18 (2025)

Abstract

The quantity of sildenafil in human plasma was determined using a simple and incredibly accurate LC-ESI-MS approach. Thermo, Hypurity Cl8, 100*4.6 mm, 5µm, was injected with sildenafil and sildenafil d3 (internal standard), which were extracted from the plasma using the solid phase extraction technique. Acetonitrile: 0.1% Formic Acid (70:30, v/v) made up the mobile phase, which was supplied isocratically at a flow rate of 0.6 mL/min. The run time was 3 minutes, and the MRM mode for sildenafil and internal standard sildenafil d3 was found at m/z 475.2→283.2 and 478.2→283.2. The calibration curve for sildenafil was linear between 25.0 and 2000 ng/mL, and the detection limit was 25.0 ng/mL. A validated LC-MS/MS method was used to determine drug concentrations.

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