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Phytochemicals and antioxidant capacity of selected Rutaceae species used in Sri Lanka

Author Affiliations

  • 1Department of Crop Science, Faculty of Agriculture, University of Ruhuna, Sri Lanka
  • 2Industrial Technology Institute, Baudhdhaloka Mawatha, Colombo 7, Sri Lanka
  • 3Department of Crop Science, Faculty of Agriculture, University of Ruhuna, Sri Lanka
  • 4Industrial Technology Institute, Baudhdhaloka Mawatha, Colombo 7, Sri Lanka

Res.J.chem.sci., Volume 10, Issue (3), Pages 48-52, October,18 (2020)


Pas-pangiri is a collection of five different Rutaceae species known as Citrus aurantium Linn, Citrus aurantifolia (Christm. & Panzer) Swingle, Citrius sinensis Linn, Atalantica ceylanica (Am.) Oliver and Citrus reticulata Blanco. They exhibit many beneficial effects such as anti-bacterial,anti-fungal, antiseptic, anti-inflammatory, etc. Even though these Rutaceae species have extensively used in different medicinal systems, scientific information on phytochemical contents and antioxidant capacity are scattered or lacking. Hence, scientific experiments were done to investigate the phytochemicals and antioxidant potential of these five Rutaceae species grown in Sri Lanka. Both aqueous leaf extracts and leaf oil of all five Rutaceae species were subjected to Thin Layer Chromatographic (TLC) analysis and phytochemical screening using standard protocols. In addition, total phenolic content, total flavonoid content and DPPH free radical scavenging ability were investigated. Preliminary phytochemical screening revealed that presence of phenols, flavonoids, tannin, saponins, steroid glycosides, coumarins, terpenoid and alkaloids in all tested plant species except A. ceylanica. TLC fingerprint profiles of C. sinensis and C. reticulata are similar to each other. However, TLC fingerprint profiles of other tested Rutaceae species were different to each other. Further, C. reticulata exhibited the highest amount of phenols and flavonoids followed by C. aurantifolia, C. aurantium, C. sinensis and A. ceylanica respectively. Accordingly, best free radical scavenging ability was also exhibited with C. reticulata and lowest ability was exhibited with A. ceylanica. Therefore, tested Rutaceae species can be utilized in pharmaceutical and food industries in future.


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