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Synthesis of Nickel Hydroxide Nanoparticles by Reverse Micelle Method and its Antimicrobial Activity

Author Affiliations

  • 1Material Science and Nano materials Lab, Dept. of Chemistry, Ch. Devi Lal University, Sirsa 125 055, INDIA
  • 2 Microbial Lab, Dept. of Biotechnology, Ch. Devi Lal University, Sirsa 125 055, INDIA

Res.J.chem.sci., Volume 1, Issue (9), Pages 42-48, December,18 (2011)


Rod shaped Ni(OH)2 nanoparticles of average size 10-20 nm were synthesized in Triton/water/cyclohexane/Polyvinyl pyrolidone quaternary microemulsion by reverse micelle method in different water to surfactant ratio. Nanoparticles were characterized by XRD, TEM, FTIR and DSC techniques. Antibacterial activity of Ni(OH)2 nanoparticles at different concentrations (0.05 to 1.2 mg/ml) were tested on three bacterial strains i.e. E. coli, P. aerugenosa and S. aureus and further compared with seven well known antibiotics. A significant increase in the antibacterial activity of nickel hydroxide nanoparticles have been observed with the increase in concentration of nanoparticles. Optimum concentration for inhibiting growth of bacterial cell was found to be 0.4 mg/ml. It is believed that Ni(OH)2 nanoparticles due to its small size penetrated the bacterial cell membrane and binds to functional groups of proteins, resulting in denaturation of protein and also believed to have caused damage to the bacterial cell by interacting with phosphorous and sulphur containing compounds such as DNA causing death of bacterial cell.


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