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Determination of Paracetamol from different Medicinal sample Colorimetrically and its FTIR study

Author Affiliations

  • 1Department of Chemistry, Brijlal Biyani Science College, Amravati, Maharashtra, India

Res. J. of Pharmaceutical Sci., Volume 14, Issue (2), Pages 27-37, December,30 (2025)

Abstract

Paracetamol (acetaminophen or para-hydroxyacetanilide) is a non-opioid analgesic and antipyretic agent used to treat fever and mild to moderate pain. Paracetamol is available in oral, suppository, and intravenous forms. In some formulations, paracetamol is combined with the opiate codeine, sometimes referred to as co-codamol (BAN) and Panadeine. Paracetamol is also combined with other opioids such as dihydrocodeine, referred to as co-dydramol (British Approved Name (BAN)), oxycodone or hydrocodone. Another very commonly used analgesic combination includes paracetamol in combination with propoxyphene napsylate. A combination of paracetamol, codeine, and the doxylamine succinate is also available. Paracetamol is sometimes combined with phenylephrine hydrochloride. Sometimes a third active ingredient, such as ascorbic acid, caffeine, chlorpheniramine maleate, or guaifenesin is added to this combination. Present study deals with determination of paracetamol from different medicinal sample Macfast 500, SumoL 650, LANOL ER 650, P-250, Arden 650, Calpal 500, Polo-650, Wellpar 500, MEDOMOL 650 and Dolo 650 Colorimetrically and FTIR study of Dolo 650Paracetamol medicinal sample. Determination of paracetamol from different medicinal sample colorimetrically involves preparation of standard paracetamol solution, paracetamol medicinal sample solution and resorcinol solution. Different systems were prepared. Absorbance of standard paracetamol system was taken at different wavelength. And λmax was calculated. Which was 530 nm. Absorbance of standard paracetamol systems were taken at 530 nm and Calibration plot was prepared. Then absorbance of paracetamol medicinal systems were taken at 530 nm and concentration of paracetamol present in different medicinal sample were calculated from calibration plot. Paracetamol with resorcinol gave azodye and the concentration of paracetamol was investigated colorimetrically. The results thus obtained are in good agreement with the quoted values. The method is simple, rapid and precise. FTIR spectra of Dolo 650 Paracetamol medicinal sample is obtained at room temperature by using an FTIR Spectrophotometer – Perkin Elmer – Spectrum RX-IFTIR. The spectra is collected in a range from 400 to 4000 cm-1. Interpretation of FTIR spectra of Paracetamol shows various groups such as C-H bending - Aromatic Compound, C-N stretching - Amine, O-H stretching - alcohol, O-H bending - Alcohol, O-H bending - Phenol, N-H stretching - Secondary amine, S-H stretching - Thiol, C=C stretching - Conjugated alkene, C≡C stretching - Alkyne, C≡N stretching – Nitrile.

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