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Evaluation of the repellent activity of Azadirachta indica oil (meliaceae) from Cameroon, and its preparations on Anopheles gambiae, Culex quinquefasciatus and Aedes albopictus

Author Affiliations

  • 1Laboratory of Galenic Pharmacy – Université des Montagnes, Bangangté / Cameroon
  • 2Laboratory of Galenic Pharmacy – Université des Montagnes, Bangangté / Cameroon
  • 3Laboratory of Pharmacognosy - Université des Montagnes, Bangangté / Cameroon
  • 4Laboratory of Parasitology- Yaoundé 1 University / Cameroon

Res. J. of Pharmaceutical Sci., Volume 11, Issue (1), Pages 1-12, June,30 (2022)


The emergence of synthetic insecticides resistant genes in mosquitoes has prompted researchers to develop vector control tools based on the use of natural repellent plants. Thus, in this study, the repellent activity of Azadirachta indica seed oil (Neem oil) as well as that of three of its preparations (candle, cream and lotion all at 30%), were evaluated on experimental populations and on wild populations of Anopheles gambiae s.l., Culex quinquefasciatus and Aedes albopictus. The repellent effect was assessed in the laboratory, and the percentage of protection duration were noted on the field. Under laboratory condition, Neem oil resulted in 48% repulsion against Culex quinquefasciatus, 10% against Anophelès gambiae and 6% vis-à-vis Aedes albopictus; with the lotion, there was 46% repulsion against Anopheleles gambiae, 25% against Culex quinquefasciatus and 10% against Aedes albopictus. The repellent effect of the cream, was more pronounced on Culex quinquefasciatus with 22% repulsion, versus 19% against Aedes albopictus and 3% against Anopheles gambiae. Candles showed 8% and 14% repulsion respectively against Culex quinquefasciatus and Anopheles gambiae. In the field, Culex quinquefasciatus was less sensitive to Neeem oil with a repellency rate of 89% and a protection time of 1hour and 30 minutes. He was even less sensitive to lotion with 30 minutes of protection for a repellency rate of 19%. No bite of Anopheles gambiae s.l. nor of Mansonia was recorded during the entire duration of the test by the volunteers who used Neem oil, that means a repellency rate of 100%. The oily solution (lotion) also recorded a 100% repellency rate against Mansonia sp and Anopheles gambiae for 2 hours 30mn and 3 hours respectively. Thus, Neem oil from Cameroon and its 30% lotion can be used as alternatives to synthetic insecticides in reducing mosquito-human contact and in reducing the density of vector populations.


  1. Mandal S. (2011)., Repellent activity of Eucalyptus and Azadirachta indica seed oil against the filarial mosquito Culex quinquefasciatus Say (Diptera: Culicidae) in India., Asian Pacific J of Tropical Biomed.; Suppl 1: S109-S112.
  2. Isman M.B, Koul O, Arnason J.T, Stewart J. and Salloum G.S. (2012)., Develloping a Neem-based insecticide for Canada., Memoirs of the Entomological Society of Canada.; 123-Suppl 159: S39-46.
  3. Biwole M. Cameroon (2017)., Far - north - the Neem tree, a tree with multiple services., The voice of the peasant. [09/06/2017]; (310): Available at url:
  4. Abiy E, Teshome GM, Meshesha B and Girmay M. (2015), Reppelent efficacity of DEET, MyggA, Neem (azadirachta indica) oil and chinaberry (Mélia azedarach) oil against Anopheles arabiensis, the principal malaria vector in Ethiopia., Malarial J.; (14), 187-193.
  5. Dua VK, Pandey AC, Raghavendra K, Gupta A, Sharma T and AP Dash (1995)., Repellent action of Neem cream against mosquitoes., Indian J malariol.; 32(2), 47-53.
  6. Bashigue V. (2013)., Ethnobotanical, biological and chemical study of plants known to be antimalarial in Lubumbashi in DR Congo., Unpublished Pharmacy Thesis: Univ Lubumbashi; 80p.
  7. Kandji N. (2001)., Study of the chemical composition and quality of vegetable oils consumed in Senegal., Pharmacy thesis: Dakar;. 99p.
  8. Practical biochemistry test, lipid indices (2016)., Cheikh Anta Diop University [cited 2016/11/30]., Available at URL:
  9. National Institute for Public Health Surveillance (2016)., Weekly epidemiological bulletin: Sanitary recommendations for travelers 2016., France: invs; 2016.
  10. Council of Europe (2014)., European Pharmacopoeia., 8th edition Volume 1.
  11. Dally L, Coulibaly S and Agnimel H, (2007)., Bamba M. Formulation, galenic, toxicological control and biogenic test of a cream with healing activity based on fresh leaves of Baphia Nitida., J. Sci. Pharm. Biol .; 8(1), 33-40.
  12. Allo O, Blanc P and Delmasso M.A. (2013)., Galenic 2013 pharmacy BP, . 3rd edition, pharmacy preparer
  13. National Institute for Industrial Environment and Risks (2011)., Classification of candles and incense according to emissions of volatile. Organic compounds and particles in indoor air., Second preliminary report for the labeling of consumer products. Paris: INERIS;
  14. Bigoga J, Saahkem P, Ndindeng S, Ngondi J, Nyegue M, Oben J et al. (2013)., Larvicidal and Repellent potential of chenopodium ambrosioides Linn Essential oil against Anopheles gambiae s. l. Giles (Diptera: culcidae)., The Open Entomology J. (7), 16-22.
  15. World Health Organization (2009)., Guidelines for efficacy testing of mosquito repellents for human skin.,
  16. Sagoua W (2009)., Synergistic study of the coupling of the Lacto peroxidase system with other active natural molecules with antifungal properties for the improvement of the fresh preservation of bananas., Agronomic Sciences: Avignon; 2009. 219p.
  17. Adjanohoun E.J. et al. (1986)., Traditional medicine and Pharmacopoeia Contribution to ethnobotanical and floristic studies in Togo., Agency for Cultural and Technical Cooperation. 1, 101, 219, 359.
  18. Agbizounon A. (2010)., Efficacy tests of vegetable oil extracts on Anophelès gambiae Giles and Culex quinquefasciatus Say resistant to pyrethroids [brief]., Environment: Abomey; 57p.
  19. Tahiri A, Adjé A and Amusant N. (2011)., Pesticide effect and screening of extracts of Azadirachta indica (A.) Juss. on the termite Macrotermes bellicosus Rambur., Woods and forests of the tropics. 310(4), 79-88.
  20. Formad environment (2013)., Neem (azadirachta indica)., [cited 2016-11-21] Available at URL: