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Thin Layer Chromatographic investigation on leave of Leucas Aspera extracted in Ethanol and Dichloromethane

Author Affiliations

  • 1Arya Vidyapeeth College, Gopinath Nagar, Guwahati, Assam, INDIA

Int. Res. J. Biological Sci., Volume 4, Issue (7), Pages 69-72, July,10 (2015)


The state of Assam has an abundance of medicinal plants known to the native people. Luecas aspera is used by rural and tribal population in Assam in various problems like dysentery, diarrhea, sinusitis, headache, tonsil etc. For thin layer chromatographic study the leaves of Leucas aspera are extracted in ethanol and dichloromethane. For thin layer chromatography, glass plate and precoated silica gel sheets were used. Twenty nine different solvent systems were prepared to develop thin layer chromatographic plate. Four visualizing reagents, viz, Iodine, H2SO4, NH3 solution, KOH solution in methanol were used to separate different spots in thin layer chromatographic plate. Dichloromethane was proved as better solvent to extract different compounds of Luecas aspera.


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