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Genomic characterization of multidrug-resistant Vibrio Cholerae O1 El Tor strains across diverse Indian geographic regions

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Author Affiliations

  • 1MATS School of Sciences, MATS University, Raipur, Chhattisgarh, India
  • 2MATS School of Sciences, MATS University, Raipur, Chhattisgarh, India
  • 3Department of Environmental Science, Sant Gahira Guru Vishwavidyalaya Sarguja, Ambikapur, Chhattisgarh, India

Int. Res. J. Biological Sci., Volume 14, Issue (2), Pages 37-45, May,10 (2025)

Abstract

Cholera poses a major health risk in several countries worldwide, even in the present century. Hence, it is very important to develop new therapeutic and diagnostic methods for restricting the transmission of the disease. Continued research in the field is necessary for understanding how the dynamics of bacterial virulence, pathogenesis and survival strategies keep changing. In the present study, a total of 20 clinical Vibrio cholerae O1 four strains from each year, from all over the country, were selected from different parts of India were investigated to analyze their genotypic and phenotypic dynamicity. The strains studied mostly used Ogawa and T-27 as the prevalent phage type. The study revealed an alarming situation of the emergence of Multidrug resistance. These strains from India emerged in the form of Haitian ctxB and Haitian tcpA. This is quiet alarming to note the shifting of genotype in V. cholerae strains in India. PFGE analysis showed that overall PFGE similarity between the strain about 80% with fourteen different patterns. Additionally, rabbit ileal loop assay showed the toxicity of V. cholerae strains. Overall, the study showed the emergence of multidrug resistance V.choleraehybrid strain circulating throughout the country.

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