Isolation, identification and screening of PGPRs and evaluation of their effectiveness on pigeon pea growth with regard to disease complex management
Author Affiliations
- 1Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India
- 2Department of Botany, Aligarh Muslim University, Aligarh, Uttar Pradesh, India
Int. Res. J. Biological Sci., Volume 14, Issue (2), Pages 12-22, May,10 (2025)
Abstract
Plant disease is major problem to global food production. Employments of excess of chemical pesticides in the management of plant disease became major problem to main table agronomics. PGPRs is usually known for plant protection as well as for enhancing crop productivity. The implementation of PGPR can reduce the requirement of pesticides and chemical fertilizers which is very important for sustainable agriculture. This is proved by the researchers PGPRs enhancing the crop yield via direct and indirect mechanism of plants. Pseudomonas fluorescnes is an important PGPR which have great importance in systemic resistance ,plant growth promotion, and biotic management of plant disease and plant growth promotion. Farmers applied large amount of chemical pesticides and food production. PGPR are most important agents in the reduction of disease and increment of growth and yield of plants. Seven strains of Pseudomonas fluorescnes were separated from the soil of pigeon pea field. Pseudomonas fluorescens have significant importance in the increment of plant health and growth. The current study aim to isolated and identify the Pseudomonas fluorescens from the soil their role in the control of wilt disease of pigeon pea. PGPR suppresses plant disease by production of IAA, sulphur, phosphorus, and siderophores. Pseudomonas fluorescens significantly control the plant pathogens. Pseudomonas fluorescens produced the solubilized phosphorus, potassium and fix the atmospheric nitrogen. Pseudomonas fluorescens not only protected plant from pathogens but also enhanced the soil fertility. PGPRs are the important microorganism produced the molecules which enhanced production plant hormone, growth and development.
References
- Gupta, S., Didwania, N., & Srinivasa, N. (2020)., Role of Biofertilizer in Biological Management of Fungal Diseases of Pigeon Pea [(Cajanus cajan) (L.) Millsp.]., Springer. pp. 205-217.
- Siddiqui, S., Siddiqui, Z. A., & Ahmad, I. (2005)., Evaluation of fluorescent Pseudomonads and Bacillus isolates for the biocontrol of a wilt disease complex of pigeonpea., World Journal of Microbiology and Biotechnology, 21(5), 729-732.
- Hasan, A. (1984)., Synergism between Heterodera cajani and Fusarium udum attacking Cajanus cajan., Nematologia Mediterranea, 12, 159–162.
- Siddiqui, Z. A., & Mahmood, I. (1999)., Effect of Heterodera cajani and Meloidogyne incognita with Fusarium udum and Bradyrhizobium japonicum on the wilt disease complex of pigeonpea., Indian Phytopathology, 52(1), 66-70.
- Ciftci, C., Tekdal, D., & Cetiner, S. (2021)., The importance of plant growth–promoting rhizobacteria for plant productivity., Abiotic Stress and Legumes, 7(9), 69-80.
- Sun, W., Shahrajabian, M. H., & Soleymani, A. (2024)., The Roles of Plant-Growth-Promoting Rhizobacteria (PGPR)-Based Biostimulants for Agricultural Production Systems., Plants, 13(5), 613.
- Bhat, M. A., Mishra, A. K., Jan, S., Bhat, M. A., Kamal, M. A., Rahman, S., & Jan, A. T. (2023)., Plant growth promoting rhizobacteria in plant health: a perspective study of the underground interaction., Plants, 12(3), 629.
- Karabulut, F., Khan, T., Shafi, N., & Parray, J. A. (2023)., Amelioration of biotic stress by using rhizobacteria for sustainable crop produce., Elsevier. pp. 19-42.
- Chaudhary, P., Singh, S., Chaudhary, A., Sharma, A., & Kumar, G. (2022)., Overview of biofertilizers in crop production and stress management for sustainable agriculture., Frontiers in Plant Science, 13, 930340.
- Teja, A. R., Leona, G., Prasanth, J., Yatung, T., Singh, S., & Bhargav, V. (2023)., Role of plant growth–promoting rhizobacteria in sustainable agriculture., In Advanced Microbial Technology for Sustainable Agriculture and Environment (pp. 175-197). Academic Press.
- Sharma, I. P., Chandra, S., Kumar, N., & Chandra, D. (2017)., PGPR: heart of soil and their role in soil fertility., Agriculturally Important Microbes for Sustainable Agriculture: Volume I: Plant-soil-microbe nexus, 51-67.
- Chen, X. H., Koumoutsi, A., Scholz, R., Schneider, K., Vater, J., Süssmuth, R., ... & Borriss, R. (2009)., Genome analysis of Bacillus amyloliquefaciens FZB42 reveals its potential for biocontrol of plant pathogens., Journal of biotechnology, 140(1-2), 27-37.
- El-Sayed, W. S., Akhkha, A., El-Naggar, M. Y., & Elbadry, M. (2014)., In vitro antagonistic activity, plant growth promoting traits and phylogenetic affiliation of rhizobacteria associated with wild plants grown in arid soil., Frontiers in microbiology, 5, 651.
- Siddiqui, Z. A. (2006)., PGPR: prospective biocontrol agents of plant pathogens., PGPR: biocontrol and biofertilization, 111-142.
- Antoun, H. and Prévost, D. (2006)., Ecology of plant growth promoting rhizobacteria., PGPR: Biocontrol and biofertilization, 1-38.
- Weller, D. M., & Thomashow, L. S. (1993)., , Use of rhizobacteria for biocontrol.
- Kumar, H., Bajpai, V. K., Dubey, R. C., Maheshwari, D. K., & Kang, S. C. (2010)., Wilt disease management and enhancement of growth and yield of Cajanus cajan (L) var. Manak by bacterial combinations amended with chemical fertilizer., Crop Protection, 29(6), 591-598.
- Weller, D. M., & Thomashow, L. S. (1993)., Use of rhizobacteria for biocontrol., Current Opinion in Biotechnology, 4(3), 306-311.
- Hoffland, E., Hakulinen, J., & Van Pelt, J. A. (1996)., Comparison of systemic resistance induced by avirulent and nonpathogenic Pseudomonas species., Phytopathology, 86(7), 757-762.
- Wei, G., Kloepper, J. W., & Tuzun, S. (1996)., Induced systemic resistance to cucumber diseases and increased plant growth by plant growth-promoting rhizobacteria under field conditions.,
- Kurabachew, H., & Wydra, K. (2013)., Characterization of plant growth promoting rhizobacteria and their potential as bioprotectant against tomato bacterial wilt caused by Ralstonia solanacearum., Biological control, 67(1), 75-83.
- Kuarabachew, H., Assefa, F., & Hiskias, Y. (2007)., Evaluation of Ethiopian isolates of Pseudomonas fluorescens as biocontrol agent against potato bacterial wilt caused by Ralstonia (Pseudomonas) solanacearum., Acta Agriculturae Slovenica, 90(2), 125-135.
- Kumar, V., Sharma, N., & Kansal, S. (2019)., Characterization of potential PGPRs isolated from rhizoshere of wheat from trans-Himalayas and their efficacy on seed germination and growth promotion of wheat under net house conditions., Journal of Plant Development Sciences, 11(3), 121-131.
- Holt, J. G., Krieg, N. R., Sneath, P. H., Staley, J. T., & Williams, S. T. (1994)., Bergey’s Manual of Determinative bacteriology, 9th edn., Williams and Wilkins, Baltimore, USA, 749-755.
- Zhang, B., Phetsang, W., Stone, M. R. L., Kc, S., Butler, M. S., Cooper, M. A., ... & Blaskovich, M. A. (2023)., Synthesis of vancomycin fluorescent probes that retain antimicrobial activity, identify Gram-positive bacteria, and detect Gram-negative outer membrane damage., Communications Biology, 6(1), 409.
- Bauer, A. W. (1996)., Antibiotic susceptibility testing by a standardized single disc method., Am. J. of Clinc. Path., 45(4) 149-158.
- Antoun, H., Bordeleau, L. M., & Prevost, D. (1982)., Strain identification in Rhizobium meliloti using the antibiotic disk susceptibility test., Plant and soil, 66, 45-50.
- Lorck, H. (1948)., Production of hydrocyanic acid by bacteria., Physiologia Plantarum, 1(2), 142-146.
- Penrose, D. M., & Glick, B. R. (2003)., Methods for isolating and characterizing ACC deaminase‐containing plant growth‐promoting rhizobacteria., Physiologia plantarum, 118(1), 10-15.
- Kotasthane, A. S., Agrawal, T., Zaidi, N. W., & Singh, U. S. (2017)., Identification of siderophore producing and cynogenic fluorescent Pseudomonas and a simple confrontation assay to identify potential bio-control agent for collar rot of chickpea., Biotech, 7(2), 137.
- Riker, A. J., & Riker, R. S. (1936)., Introduction to research of Plant diseases., Formerly Research Associate in Plant Pathology.
- Siddiqui, Z. A., & Mahmood, I. (1995)., Biological control of Heterdora cajani and Fusarium udum by Bacillus subtilis, Bradhyrhizobium japonicum and Glomus fasciculatum on pigeon pea., Fundamental and Applied Nematology, 18(6), 559-566.
- Wu, Y., Xiao, S., Qi, J., Gong, Y., & Li, K. (2022)., Pseudomonas fluorescens BsEB-1: an endophytic bacterium isolated from the root of Bletilla striata that can promote its growth., Plant Signaling & Behavior, 17(1), 2100626.
- Holbrook, C. C. Knauft, D. A. & Dickson, D. W. (1983)., A technique for screening peanut for resistance to Meloidogyne arenaria. Plant Disease, 67(9), 957., undefined
- Taylor, A. L. & Sasser, J. N. (1978)., Biology, Identification and Control of Root-Knot Nematodes (Meloidogyne Species)., A Cooperative Publication of the Department of Plant Pathol. pp. 111.
- Aron, D. (1949)., Copper enzymes isolated chloroplasts, polyphenoloxidase in Beta vulgaris., Plant Physiol, 24(1), 1-15.
- Bates, L. S., Waldren, R. A., & Teare, I. D. (1973)., Rapid determination of free proline for water-stress studies., Plant and soil, 39, 205-207.
- Kaur, C., & Kapoor, H.C. (2002)., Anti-oxidant activity and total phenoliccontent of some Asian vegetables., Int J. Food Sci Technol, 37(2), 153–161.
- Dukare, A., & Paul, S. (2021)., Biological control of Fusarium wilt and growth promotion in pigeon pea (Cajanus cajan) by antagonistic rhizobacteria, displaying multiple modes of pathogen inhibition., Rhizosphere, 17, 100278.
- Bhardwaj, D., Ansari, M. W., Sahoo, R. K., & Tuteja, N. (2014)., Biofertilizers function as key player in sustainable agriculture by improving soil fertility, plant tolerance and crop productivity., Microbial cell factories, 13, 1-10.
- Lalande, R., Bissonnette, N., Coutlée, D., & Antoun, H. (1989)., Identification of rhizobacteria from maize and determination of their plant-growth promoting potential., Plant and Soil, 115, 7-11.
- Glick, B. R. (1995)., The enhancement of plant growth by free-living bacteria., Canadian J of Microbiol, 41(2), 109-117.
- Whipps, J. M. (2001)., Microbial interactions and biocontrol in the rhizosphere., J of Exp Bot, 52(1), 487-511.
- Ahmad, F., Ahmad, I., & Khan, M. S. (2008)., Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities., Microbiol Res, 163(2), 173-181.
- Bhattacharyya, P. N., & Jha, D. K. (2012)., Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture., World J of Microbiol and Biotechnol, 28, 1327-1350.
- Mirza Sajjad, M., Ahmad, W., Latif, F., Haurat, J., Bally, R., Normand, P., & Malik, K. A. (2001)., Isolation, partial characterization, and the effect of plant growth-promoting bacteria (PGPB) on micro-propagated sugarcane in vitro., Plant and soil, 237, 47-54.
- Kloepper, J.W., Lifshitz, R. and Zablotowicz, R.M. (1989)., Free-living bacterial inocula for enhancing crop productivity., Trends in Biotechnol, 7(2), 39-43.
- Arshad, M., & Frankenberger Jr, W. T. (1997)., Plant growth-regulating substances in the rhizosphere: microbial production and functions., Adv in Agron, 62, 45-151.
- Latha, T. K. S., & Rajeswar, E. (2000)., Management of root-rot disease complex through antagonists and chemicals., Indian Phytopathol, 53(2), 216-218.
- Siddiqui, S., Siddiqui, Z. A., & Ahmad, I. (2005)., Evaluation of fluorescent Pseudomonads and Bacillus isolates for the biocontrol of a wilt disease complex of pigeonpea., World Jof Microbiol and Biotechnol, 21, 729-732.
- Prasad, R. D., & Rangeshwaran, R. (2000)., Effect of soil application of a granular formulation of Trichoderma harzianum on Rhizoctonia solani incited seed rot and damping-off of chickpea., J of Mycol and Plant Pathol, 30(2), 216-220.
- Siddiqui, I. A., & Shaukat, S. S. (2002)., Resistance against the damping‐off fungus Rhizoctonia solani systemically induced by the plant‐growth‐promoting rhizobacteria Pseudomonas aeruginosa (IE‐6S+) and P. fluorescens (CHA0)., J of Phytopathol, 150, (9), 500-506.
- Ali, S., Hameed, S., Shahid, M., Iqbal, M., Lazarovits, G., & Imran, A. (2020)., Functional characterization of potential PGPR exhibiting broad-spectrum antifungal activity., Microbiol Res, 232, 126389.
- Kloepper, J. W., Leong, J., Teintze, M., & Schroth, M. N. (1980)., Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria., Nature, 286(5776), 885-886.
- Weller, D. M. (1988)., Biological control of soilborne plant pathogens in the rhizosphere with bacteria., Annual review of phytopathol, 26(1), 379-407.
- Siddiqui, Z. A. (2006)., PGPR: prospective biocontrol agents of plant pathogens., PGPR: biocontrol and biofertilization, pp.111-142.
- Siddiqui, Z. A. & Shakeel, U. (2006)., Wilt Disease Complex of Pigeonpea in Green House Assay and under Pot Condition., Plant Pathol J., 5(1), 99-105.
- Bagnasco, P., De La Fuente, L., Gualtieri, G., Noya, F., & Arias, A. (1998)., Fluorescent Pseudomonas spp. as biocontrol agents against forage legume root pathogenic fungi., Soil Biol and Biochem, 30(10-11), 1317-1322.
- Karabulut, F., Khan, T., Shafi, N., & Parray, J. A. (2023)., Amelioration of biotic stress by using rhizobacteria for sustainable crop produce., In Rhizobiome (pp. 19-42). Academic Press.
- Chauhan, V., Mazumdar, S., Pandey, A., & Kanwar, S. S. (2023)., Pseudomonas lipopeptide: An excellent biomedical agent., MedComm–Biomaterials and Appl, 2(1), e27.
- Manjula, A., Gautam, A. K., & Kumar, A. (2024)., Trichoderma as Potential Biocontrol Agenton Diseases of Soybean (Glycine max L.): A Comprehensive Review., Biol Forum – An Inte J, 16(3), 242-247.