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Standardization of DNA extraction method of milk samples and development of PCR-based method of adulteration detection in milk

Author Affiliations

  • 1Guru Ghasidas University, Bilaspur, CG, India

Res. J. Forensic Sci., Volume 13, Issue (2), Pages 11-15, July,29 (2025)

Abstract

Milk and dairy products are generally nutritious foods that are widely consumed by the public and are an essential component of the diet for specific consumer categories, especially youngsters and pregnant women. Due to its significant value and popularity, fraud in the dairy business has become widespread. Species identification using DNA-based techniques is especially helpful for the analysis of commercial dairy products because all dairy products contain DNA derived from physical animal cells, which is stable and recoverable for polymerase chain response (PCR) analysis even after thermal treatment and other forms of processing. The most common way to identify differences in dairy products is grounded on PCR, given its low limits of discovery, strong particularity, and high perceptivity. In this work DNA extraction was done from cow, goat, buffalo, and sheep by using PCR amplification with two primer UMP and cytochrome B successfully.

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