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Determination of divalent Mercury in environmental samples using 1, 5-diphenyl-3-thiocarbazone: with modified, ultrasensitive, direct Spectrophotometric method

Author Affiliations

  • 1Department of Environmental Science and Research Centre, K.R.T. Arts, B.H. Commerce and A.M. Science College, Nashik, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India
  • 2Departments of Microbiology, K.R.T. Arts, B.H. Commerce and A.M. Science College, Nashik, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India
  • 3Department of Botany, K.R.T. Arts, B.H. Commerce and A.M. Science College, Nashik, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India

Res. J. Recent Sci., Volume 6, Issue (3), Pages 8-12, March,2 (2017)


The present research investigated that, the analytical reagent,1,5-diphenyl-3 thiocarbazone dissolves in acetone in strongly acidic and 1,4 dioxane media which reacts with mercury (II) to produce orange-red coloured complex at pH 2 (0.2N sulphuric acid). The complex was showing maximum absorbance at 488 nm. Therefore, further analytical parameters were performed at 488nm. The reaction occurs within a minute and absorbance remains unchanged for 24 hrs. The analytical parameters like, effect of metal concentration, reagent concentration, acidity, interference by other metal ions, were investigated. The Lambert-Beer’s law followed within a range of 0.1-25 µgml-1of Hg(II). The stoichiometric ratio of a reagent with the reacting metal is 1:2 (Mercury:Dithiazone). The molar absorptivity was found to be 2.4×104 lmol-1cm-1 and that of specific absorptivity was 0.015µg of mercury (II)/cm2. The selected method is modified, ultrasensitive and is successfully applied for estimation of divalent mercury from polluted water samples.


  1. Krabbenhoft David P. and Rickert David A. (1995)., Mercury Contamination of Aquatic Ecosystems., U.S. Geological Survey, FS-216-95, 1-4.
  2. Martin S. (2009)., Human Health Effects of Heavy Metals., Environmental Science and Technology Briefs for Citizens, (CHSR), 15, 1-6.
  3. Jarup Lars (2003)., Hazards of heavy metal contamination., Oxford Journals, Medicine and health, British medical bulletin, 68(1), 167-182.
  4. Kudesia V.P. (1988)., Toxicity of metals., in: Air Pollution, Pragall Prakashan, Meerut, 178-199.
  5. De A.K. (1989)., Environmental Chemistry., Wiley Eastern Limited, New Delhi, 2nd edition, 75-271.
  6. Irving H.M.N.H. and Iwantscheff G. (1980)., The analytical application of Dithizone., J. CRC Critical reviews of analytical Chemistry, 8(4), 321-366.
  7. Yano T., Ide S., Tobeta Y., Kobayashi H. and Ueno K. (1976)., Analytical applications of organic reagents in hydrophobic gel media—II: Selective preconcentration of mercury(II) with dithizone or thiothenoyltrifluoroacetone gel., Talanta, 23(6), 457-459.
  8. Manzoori J.L., . Sorouraddin M.H. and Haji Shabani A.M. (1998)., Determination of mercury by cold vapour atomic absorption spectrometry after preconcentration with dithizone immobilized on surfactant-coated alumina., J. Anal. At. Spectrom.,13(4), 305-308
  9. Jamaluddin A. and Alam Md. (2003)., A rapid spectrophotometric method for the determination of mercury in environmental, biological, soil and plant samples using diphenylthiocarbazone., Spectroscopy, 17(1), 45-52.
  10. Berman E. (1980)., Toxic Metals and Their Analysis, Heyden International topics in Science Series., John Wiley and Sons, Canada, 1-293.
  11. Fergusson J.E.(1990), The Heavy Elements: Chemistry, Environmental Impact and Health Effects, Pergamon Press, Oxford, 85-547., undefined, undefined
  12. Jones J.B. (1984)., Developments in the measurement of trace metal constituents in food., Analysis of Food Contaminants, J. Gilbert, ed., Elsevier, Oxford, 157-205.
  13. Magos L. (1984). Mercury, in:, Hazardous Metals in Human Toxicology, Part B., A. Vercruysse, ed., Elsevier, New York, 171-194.
  14. Sandell E.B. (1950)., Colorimetric Determination of Traces of Metals., 3rd edn, Interscience, New York, 269.
  15. Allport N.L. and Brocksopp J.E. (1963)., Colorimetric Analysis., Chapman and Hall Ltd., London, 2, 184-193.
  16. Jackson M.L. (1965)., Soil Chemical Analysis., Prentice Hall Inc., N.J., USA, 326-338.
  17. Christian G.D. (1986)., Analytical Chemistry., 4th edn, John Wiley and Sons, New York, 357-389.
  18. Mitra S. (2004)., Sample Preparation Techniques in Analytical Chemistry., Wiley Interscience, New Jersey, 230-233.
  19. Job P. (1928)., Formation and stability of inorganic complexes in solution., SID, 9, 113-113.
  20. Wheatley B. and Wyzga R. (1997)., Mercury as a Global Pollutant, Human Health-Issues., Kluwer, Dordrecht, The Netherlands, 56-77.
  21. You J.A. and Jones A.L. (1944)., Limits of molar ratio method., Ind. Eng. Chem. Anal. Ed., 16, 111.