International Science Community Association Research Journal of Recent Sciences 2277-2502 7 7 2018 07 2 Antioxidant and antimicrobial activities of Halocnemum strobilaceum fractions and their related bioactive molecules identification by GC/MS and HPLC 1 9 EN Saada Mariem 1 Laboratoire des Plantes Aromatiques et Médicinales, Centre de Biotechnologie de Borj-Cédria (CBBC), BP 901, 2050 Hammam-lif, Tunisia Kasmi Mariam 2 High School of Food Industry of Tunis (ESIAT), University of Carthage,Cité El Khadhra 1003, Tunisia and Laboratoire de Traitement et de Valorisation des Rejets Hydriques (LTVRH), Water Researches and Technologies Center (CERTE), University of Carthage, Tourist route Soliman, BP 273-8020 Nabeul, Tunisia Ben Jemaa Mariem 3 Laboratoire des Plantes Aromatiques et Médicinales, Centre de Biotechnologie de Borj-Cédria (CBBC), BP 901, 2050 Hammam-lif, Tunisia Ksouri Riadh 4 Laboratoire des Plantes Aromatiques et Médicinales, Centre de Biotechnologie de Borj-Cédria (CBBC), BP 901, 2050 Hammam-lif, Tunisia > Research Paper 2018 1 15 2018 07 2 Polar and apolarfractions of Halocnemum strobilaceum shoot extracts were evaluated for their phenolic contents and biological activities. HPLC and GC/MS methods have been implemented for phenolics and fatty acids content identification, respectively. Results showed that phenolic contents and antioxidant activities varied considerably according to the fraction nature. In fact, the apolar fraction contains two folds higher flavonoïd and tannin contents (1.87 and 0.83mg CE.g-1DW, respectively) compared to the polar one. In contrast, total polyphenol content was superior in the polar fraction. The important phenolics content in chloroformic extract was correlated to the best antioxidant activity such as total antioxidant activity (5.54mg GAE.g-1DW), antiradical activity against DPPH radical, & Copyright@ International Science Community Association 946;-carotene bleaching and Fe-reducing capacities with the lowest IC50 and EC50 values compared to the polar fraction. Furthermore unlike the polar fraction the chloroformic one was efficient against all pathogen strains mainly Salmonella typhimurium. The HPLC analysis showed that among twelve phenolic compounds detected only five were successfully identified (rutin hydrate p-coumaric O-coumaric rosmarinic and 3 4-dimethoxybenzoic acids). The major fatty acids identified by GC/MS were palmitic oleic and linolenic acids. The antioxidant and antimicrobial potency differences between the selected fractions was mainly attributed to different bioactive molecules contained in each fraction. Such characteristics would be of paramount importance for this halophyte valorization and use in cosmetic and pharmaceutical industries as a valuable source of antioxidant molecules.