International Science Community Association Research Journal of Recent Sciences 2277-2502 7 3 2018 03 2 Simultaneous validation of amoxicillin trihydrate and carbocisteine in pharmaceutical dosage by reverse phase high performance liquid chromatography 8 13 EN Polorigni Botolisam 1 Laboratory of forest researches, Faculty of Sciences, University of Lomé, 01BP 1515 Lomé 01-Togo Adjonou Kossi 2 Laboratory of forest researches, Faculty of Sciences, University of Lomé, 01BP 1515 Lomé 01-Togo Radji Raoufou A. 3 Laboratory of forest researches, Faculty of Sciences, University of Lomé, 01BP 1515 Lomé 01-Togo Kokou Kouami 4 Laboratory of forest researches, Faculty of Sciences, University of Lomé, 01BP 1515 Lomé 01-Togo Rele Rajan V. 1 Central Research Laboratory, Chemistry, D.G. Ruparel College, Dept. of Chemistry, Matunga, Mumbai-400 016, India Tiwatane Prathamesh P. 2 Central Research Laboratory, Chemistry, D.G. Ruparel College, Dept. of Chemistry, Matunga, Mumbai-400 016, India > Research Paper 2017 9 1 2018 03 2 For validation of amoxicillin trihydrate and carbocisteine from the formulation i.e. capsules, a RP-HPLC method is developed. The amoxicillin trihydrate and carbocisteine were resolved on a BDS hypersil C18 (150 x 4.6 mm i.d.) 5 µ particle size. The water and methanol in the ratio of 80: 20 % (v/v) constituted as mobile phase with detection wavelength as 220 nm. The column BDS hypersil C18 was found to be most suitable for chromatographic separation. The parameters for robustness were validated. The linear ranges amoxicillin trihydrate as 125 - 375 and carbocisteine at 75 - 225 & Copyright@ International Science Community Association