@Research Paper
<#LINE#>Isolation, screening, optimization and production of Anti-tumor L-Asparaginase by fungi from karwar coastal region<#LINE#>Sanjotha @G.,Sudheer I. @Manawadi <#LINE#>1-7<#LINE#>1.ISCA-RJRS-2017-021.pdf<#LINE#>Department of Biotechnology, Government Arts and Science College Karwar-581301, Karnataka, India@Department of Biotechnology, Government Arts and Science College Karwar-581301, Karnataka, India<#LINE#>27/11/2016<#LINE#>28/1/2017<#LINE#>L-asparaginase is an extracellular enzyme that converts L-asparagine to L-Aspartic acid and has gained considerable attention in the recent years. In the present study, soil samples were gathered from different areas in and around the Karwar regions, Karnataka, India. Several fungal species were isolated and identified using standard manuals, screened primarily for the production of L-Asparaginase on Czapek’s Dox medium containing L- Asparagine as main source of carbon. A total of 50 fungal cultures were isolated from soil. The fungal isolates obtained were selected by plate assay method for screening of potential L-Asparaginase production on Modified Czapek Dox’s (mCD) medium. Enzyme production was carried out by submerged fermentation process and was performed by using mCD liquid media. Quantitative enzyme assay was performed to determine the rate of hydrolysis of L-Asparagine by measuring the liberated ammonia by nesslerization. From the 50 total isolates 10 fungal cultures showed L-Asparaginase activities. The cultures were identified as Penicillum species, Basidiomycetes species. Aspergillus species, Mucor species. Fusarium species. Among the above species Aspergillus sp showed potential L-Asparaginase production (155U/ml). An attempt is made to optimize the cultural conditions for the production of potential L-Asparaginase by using submerged fermentation. Different temperature (150C, 250C, 350C, 450C) different pH (3.5, 5.5, 7.5, 9.5, and 11.5) different carbon and nitrogen source were used. The highest amount of enzyme production was observed at pH 7.5(155 U/ml) and temperature at 35°C (160 U/ml), among the various carbon sources dextrose promoted maximum enzyme activity (176 U/ml) and highest activity was obtained when nitrogen source ammonium sulphate was used (185 U/ml).<#LINE#>Jemal A., Bray F., Center M.M., Ferlay J., Ward and E. Forman D. (2011).@Global cancer statistics.@CA Cancer J Clin., 61(2):69-90. doi: 10.3322/caac.20107. Erratum in: CA Cancer J Clin. 2011 Mar-Apr; 61(2):134.@Yes$Weinberg R.A. (1996).@How cancer arises.@Scientific American, 275(3), 62-71.@Yes$Kotzia G.A. and Labrou N.E. (2007).@L-Asparaginase from Erwinia chrysanthemi 3937, Cloning, Expression and Characterization.@Journal of Biotechnology, 127(4), 657-669.@Yes$Berenbaum M.C., Ginsburg H. and Gilbert D.M. (1970)@Effects of L-asparaginaseon lymphocyte target cell reactions in Vitro.@Nature, 227(5263), 1147-1148.@Yes$Broome J.D. (1963).@L-asparaginase EC-II from Escherichia coli.Some substrate specificity characteristics.@Biochemistry 8, 3766-3772.@Yes$Wriston J. and Yellin T. (1973).@L-Asparaginase: A Review.@Adv Enzymol Relat Areas Mol Biol,  39, 185-248.@Yes$Yellin T. and Wriston J. (1973).@Purification and Properties of Guinea Pig Serum Asparaginase.@Biochemistry, 5(5), 1605-1612.@Yes$Capizzi R.L., Poole M., Cooper M.R., Richards F., Stuart J.J., Jakson D.V., White D.R., Spurr C.L., Hopkins J.O. and Muss H.B. (1984).@Treatment of poor risk acute leukaemia with sequential hig-done ARA-C and asparaginase.@Blood 63(3), 694-700.@Yes$Lubkowski J., Palm G.J., Gilliland G.L., Derst C., Röhm K.H. and Wlodawer A. (1996).@Crystal structure and amino acid sequence of Wolinella succinogenes l-asparaginase.@European Journal of Biochemistry, 241(1), 201-207.@Yes$Clementi A. (1922).@La désamidation enzymatique de l@Archives Internationales de Physiologie, 19(4), 369-398.@Yes$Gulati R., Saxena R.K. and Gupta R. (1997).@A rapid plate assay for screening L-asparaginase producing micro-organisms.@Lett Appl Microbiol, 24(1), 23-26@Yes$Dhevagi P. and poorani E. (2006).@Isolation and characterization of l asparaginase from marine actinomycetes.@international journal of biotechnology, 5, 514-520@Yes$Selvakumar N. (1991).@vanajakumar and Natarajan R.@Partial purification, characterization and anti tumor properties of l asparaginase from vibrio in bioactive compounds from microorganisms, 289-300.@Yes$Tiwari N. and Dua R.D. (1996).@purification and preliminary characterization of l asparaginase from Erwinia aroideae NRRL B- 138.@Ind. J Biochem Biophys, 33(5), 371-376.@Yes$Wade H.E., Robinson H.K. and Philips B.W. (1971).@Asparaginase and glutaminase activities of bacteria.@J.Gen Microbiol., 69(3), 299-312.@Yes$Wiame J.M., Grenson M. and Arst N.H. Jr. (1985).@Biodiversity of higher yeasts and filamentous fungi.@AdvMicrobial Physiol., 26, 1-88.@No$Pinheiro I.O., Araujo J.M., Ximenes E.C.P.A., Pinto J.C.S.  and Alves T.L.M. (2001).@Production of L-Asparaginase by zymononas mobiles strain Cp4.@Biomaterial and Diagnostic, 6, 243-244.@Yes$Saxena R.K. and Sinha U. (1981).@L-asparaginase and glutaminase activities in the culture filtrates of Aspergillus nidulans.@Cur Sci., 50, 218-219.@Yes$Imada A., Igarasi S., Nakahama K. and Isono M. (1973).@Asparaginase and glutaminase activities of micro-organisms.@J Gen Microbiol, 76(1), 85-99.@Yes$Ellis M.B. (1976).@More “dematiaceous hyphomycetes”. Commonwealth Mycological Institute, Kew, Survey.@England 507, ISBN 0-79235957-7.@Yes$Raper K.B. and Fennell D.I. (1965).@The Aspergillus, Baltimore: Williams and Wilkins.@1-686.@Yes$Lapmak Kodchakorn, Lumyong Saisamorn, Thongkuntha Sutheera, Wongputtisin Pairote and Sardsud Uraporn (2010).@L-ASPARAGINASE PRODUCTION BY BIPOLARIS SP. BR438 ISOLATED FROM BROWN RICE IN THAILAND.@Chiang Mai Journal of Science., 37, 160-164.@Yes$Hosamani R. and Kaliwal B.B. (2011).@Isolation, molecular identification and optimization of fermentation parameters for the production of L-asparaginase, an anticancer agent by Fusarium equiseti.@Int J Microbiol Res, 3(2), 108-119.@Yes$Rani S.A., Lalitha S. and Praveesh B.V. (2011).@In vitro antioxidant and anticancer activity of L-asparaginase from Aspergillus flavus (KUFS20).@Asian J. Pharm. Clin. Res., 4, 174-177.@Yes$Sarquis M.I., Oliveira E.M., Santos A.S. and Costa G.L. (2004).@Mem Inst Oswaldo Cruz.@99(5), 489-92.@No$Siddalingeshwara K.G. and Lingappa K. (2011).@Production and characterization of L-asparaginase-a tumour inhibitor.@Int J Pharm Tech Res, 3(1), 314-319.@Yes$Monica T., Lynette L., Niyonzima F.N. and Sunil S.M. (2013).@Isolation, Purification and Characterization of Fungal Extracellular L- Asparaginase from Mucor Hiemalis. J Biocatal Biotransformation 2: 2.@9, 12-14.@Yes$Kotra S.R., Prudvi N., Sada Sai K.R.A., Mannava K.K., Peravali J.B., Kumar Anmol, Sambasiva Rao K.R.S. and Pulicherla K.K. (2013). Cost effective process for the production of fungal L-asparaginases from Penicillium sps isolated from local soil sample. Mintage Journal of Pharmaceutical and Medical Sciences, 2(1), 45-50.@undefined@undefined@Yes$Jalgaonwala R.E. and Mahajan R.T. (2014).@A review: bacterial endophytes and their bioprospecting.@J Pharm Res., 4, 795-799.@Yes$Chandrasekhar A.P. (2012).@Isolation, purification and characterization of asparaginase from aspergillus species.@Int J Res Chem Environ, 2, 38-43.@Yes$Thirunavukkarasu N., Suryanarayanan T.S., Murali T.S., Ravishankar I.P. and Gummadi S.N. (2011). Mycosphere, 2(2), 147-15.@undefined@undefined@No$Mohsin S.M., Dutt Sunil, Siddalingeshwara PLNSN, Karthik K.G., Jayaramu J., Naveen M., Vishwanatha M. and Prathiba T.K.S. (2012). J Acad , Ind Res,1(4), 180-182.@undefined@undefined@No$Yadav Niharika and Sarkar Supriya (2014). Intl J Pharmaceutical Science Invention,  3(6), 32-40.@undefined@undefined@No$Thirumurugan G., Moses Jeyakumar Rajesh,  Leelavathy Rajesh, Vanapalli VSV and Rajarammohan Sivasubramanian (2011).@Effect of Inducers and Physical Parameterson the Production of L-Asparaginase Using Aspergillus Terreus.@J Bioprocess Biotechniq, 1, 1-6. doi:10.4172/2155-9821.1000110.@Yes$Dange V. and Peshwe S. (2015).@Purification and biochemical characterization of L-asparaginase from Aspergillus niger and evaluation of its antineoplastic activity.@Int. J. Sci. Res., 4(2), 564-569.@Yes$Kalyanasundaram J., Nagamuthu B., Srinivasan A., Pachayappan S. and Muthukumarasamy (2015).@Production, purification and characterization of extracellular L-asparaginase from salt marsh fungi endophytes.@World J. Pharma. Sci., 4(3), 663-667.@Yes$Baskar G. and Renganathan S. (2011).@Design of experiments andartificial neural network linked genetic algorithm formodelling and optimization of L-asparaginase production by Aspergillus terreus MTCC 1782.@Biotechnology and Bioprocess Engineering, 16(1), 50-58.@Yes$Murali T.S. (2011).@L-Asparaginase from marine derived fungal endophytes.@Mycosphere, 2(2), 147-155.@Yes$Kalyanasundaram I., Nagamuthu J., Srinivasan B., Pachayappan A. and Muthukumarasamy S. (2015).@Production, purification and characterization of extracellular L-asparaginase from salt marsh fungi endophytes.@World J. Pharma. Sci., 4(3), 663-667.@Yes$Gaffar S.A. and Shethna Y.I. (1977).@Purification and Some Biological Properties of Asparaginase from Azotobactervinelandii.@Appl Environ Microbiol., 33(3), 508-514.@Yes$Sreenivasulu V., Jayaveera K.N. and Mallikarjuna Rao (2009).@Optimization of process parameters for the production of Lasparaginasefrom an isolated fungus.@Research J.Pharmacognosy and Phytochemistry, 1(1), 30-34.@Yes
<#LINE#>Determination of divalent Mercury in environmental samples using 1, 5-diphenyl-3-thiocarbazone: with modified, ultrasensitive, direct Spectrophotometric method<#LINE#>Ghangale @Sharmila S.,Bholay @A.D.,Saler @R.S. <#LINE#>8-12<#LINE#>2.ISCA-RJRS-2017-023.pdf<#LINE#>Department of Environmental Science and Research Centre, K.R.T. Arts, B.H. Commerce and A.M. Science College, Nashik, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India@Departments of Microbiology, K.R.T. Arts, B.H. Commerce and A.M. Science College, Nashik, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India@Department of Botany, K.R.T. Arts, B.H. Commerce and A.M. Science College, Nashik, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India<#LINE#>30/11/2016<#LINE#>25/2/2017<#LINE#>The present research investigated that, the analytical reagent,1,5-diphenyl-3 thiocarbazone dissolves in acetone in strongly acidic and 1,4 dioxane media which reacts with mercury (II) to produce orange-red coloured complex at pH 2 (0.2N sulphuric acid). The complex was showing maximum absorbance at 488 nm. Therefore, further analytical parameters were performed at 488nm. The reaction occurs within a minute and absorbance remains unchanged for 24 hrs. The analytical parameters like, effect of metal concentration, reagent concentration, acidity, interference by other metal ions, were investigated. The Lambert-Beer’s law followed within a range of 0.1-25 µgml-1of Hg(II). The stoichiometric ratio of a reagent with the reacting metal is 1:2 (Mercury:Dithiazone). The molar absorptivity was found to be 2.4×104 lmol-1cm-1 and that of specific absorptivity was 0.015µg of mercury (II)/cm2. The selected method is modified, ultrasensitive and is successfully applied for estimation of divalent mercury from polluted water samples.<#LINE#>Krabbenhoft David P. and Rickert David A. (1995).@Mercury Contamination of Aquatic Ecosystems.@U.S. Geological Survey, FS-216-95, 1-4.@Yes$Martin S. (2009).@Human Health Effects of Heavy Metals.@Environmental Science and Technology Briefs for Citizens, (CHSR), 15, 1-6.@Yes$Jarup Lars (2003).@Hazards of heavy metal contamination.@Oxford Journals, Medicine and health, British medical bulletin, 68(1), 167-182.@Yes$Kudesia V.P. (1988).@Toxicity of metals.@in: Air Pollution, Pragall Prakashan, Meerut, 178-199.@No$De A.K. (1989).@Environmental Chemistry.@Wiley Eastern Limited, New Delhi, 2nd edition, 75-271.@No$Irving H.M.N.H. and Iwantscheff G. (1980).@The analytical application of Dithizone.@J. CRC Critical reviews of analytical Chemistry, 8(4), 321-366.@Yes$Yano T., Ide S., Tobeta Y., Kobayashi H. and Ueno K. (1976).@Analytical applications of organic reagents in hydrophobic gel media—II: Selective preconcentration of mercury(II) with dithizone or thiothenoyltrifluoroacetone gel.@Talanta, 23(6), 457-459.@Yes$Manzoori J.L., . Sorouraddin M.H. and   Haji Shabani A.M. (1998).@Determination of mercury by cold vapour atomic absorption spectrometry after preconcentration with dithizone immobilized on surfactant-coated alumina.@J. Anal. At. Spectrom.,13(4), 305-308@Yes$Jamaluddin A. and Alam Md. (2003).@A rapid spectrophotometric method for the determination of mercury in environmental, biological, soil and plant samples using diphenylthiocarbazone.@Spectroscopy, 17(1), 45-52.@Yes$Berman E. (1980).@Toxic Metals and Their Analysis, Heyden International topics in Science Series.@John Wiley and Sons, Canada, 1-293.@Yes$Fergusson J.E.(1990), The Heavy Elements: Chemistry, Environmental Impact and Health Effects, Pergamon Press, Oxford, 85-547.@undefined@undefined@Yes$Jones J.B. (1984).@Developments in the measurement of trace metal constituents in food.@Analysis of Food Contaminants, J. Gilbert, ed., Elsevier, Oxford, 157-205.@Yes$Magos L. (1984). Mercury, in:@Hazardous Metals in Human Toxicology, Part B.@A. Vercruysse, ed., Elsevier, New York, 171-194.@Yes$Sandell E.B. (1950).@Colorimetric Determination of Traces of Metals.@3rd edn, Interscience, New York, 269.@Yes$Allport N.L. and Brocksopp J.E. (1963).@Colorimetric Analysis.@Chapman and Hall Ltd., London, 2, 184-193.@No$Jackson M.L. (1965).@Soil Chemical Analysis.@Prentice Hall Inc., N.J., USA, 326-338.@No$Christian G.D. (1986).@Analytical Chemistry.@4th edn, John Wiley and Sons, New York, 357-389.@Yes$Mitra S. (2004).@Sample Preparation Techniques in Analytical Chemistry.@Wiley Interscience, New Jersey, 230-233.@Yes$Job P. (1928).@Formation and stability of inorganic complexes in solution.@SID, 9, 113-113.@Yes$Wheatley B. and Wyzga R. (1997).@Mercury as a Global Pollutant, Human Health-Issues.@Kluwer, Dordrecht, The Netherlands, 56-77.@Yes$You J.A. and Jones A.L. (1944).@Limits of molar ratio method.@Ind. Eng. Chem. Anal. Ed., 16, 111.@No
<#LINE#>Dissolution study and method validation of alprazolam by high performance liquid chromatography method in pharmaceutical dosage form<#LINE#>Rele @Rajan V.,Patil @Sandip P. <#LINE#>13-18<#LINE#>3.ISCA-RJRS-2017-024.pdf<#LINE#>Central research Laboratory, D.G. Ruparel College, Matunga, Mumbai-400016, India@Central research Laboratory, D.G. Ruparel College, Matunga, Mumbai-400016, India<#LINE#>12/11/2016<#LINE#>23/1/2017<#LINE#>Dissolution study was carried for alprazolam tablets containing 0.25 mg of active pharmaceutical ingredient. From the solubility study, conditions selected for paddle was 100 rpm, using 500 ml potassium dihydrogen phosphate of pH 6.8 at temperature as 37°C. These conditions are useful for % release of alprazolam coated 0.25 mg. The validation of dissolution of alprazolam was achieved on symmetyshield RP8 (150 x 3.9 mm i.d, 5 µ.) column was most suitable for dissolution study with mixture of (60:40 % v/v) of buffer and acetonitrile as mobile phase. A 0.1 % orthophosphoric acid was used as a buffer and to tri-ethylamine was used to adjust the pH 3.0 of buffer. The potassium dihydrogen phosphate of pH 6.8 was used as a diluent. The detector wavelength was set at 225 nm. Flow rate was adjusted at 0.8 ml/min. The validation of method was performed as per ICH guidelines. The linearity was found between 50- 150% with coefficient of co-relation was 0.996. The % RSD of precision was 0.66%.<#LINE#>Pérez-Lozano P., Garc´&#305;a-Montoya E., Orriols A., Miñarro M., Ticó J.R. and Suñé-Negre J.M. (2004).@Development and validation of a new HPLC analytical method for the determination of alprazolam in tablets.@Journal of Pharmaceutical and Biomedical Analysis, 34(5), 979-987.@Yes$Rele Rajan V. (2016).@Development of analytical method by RP-HPLC technique for determination of alprazolam in pharmaceutical dosage form.@International Journal of PharmTech Research,  9(9), 408-414.@Yes$Kumar Ata Kiran, Mohanakrishna A., Sudheer M., Sai Rajesh K. and Ramalingam P. (2011).@UV Spectrophotometric Method for the estimation of Alprazolam in Tablet Dosage Form.@International Journal of ChemTech Research, 3(1), 161-164.@Yes$Shaik Sarfaraz, Ch. Venkata Ramana Readdy and K.M.A. Shareef, (2014).@Method development Validation and determination of Alprazolam in its pharmaceutical dosage by 2,3- dichloro 5,6-dicyano-1,4-benzoquinone.@Journal of Chemical  and Pharmaceutical Research, 6(9), 411-418.@No$Sharma S. and Sharma M.C. (2011).@Method development and validation of UV spectrophotometric method for alprazolam in pharmaceutical dosage form using ferric chloride and indigo carmine.@European journal of applied sciences, 3(3), 81-85.@No$Rajan V. Rele and Amey Deshpande (2016).@UV spectrophotometric estimation of alprazolam by area under curve and first order derivative methods in bulk and pharmaceutical dosage form.@Der pharmacia letter, 8(5), 105-110.@No$Rajan V. Rele and Amey Deshpande (2016).@UV Spectrophotometric Estimation of Alprazolam by second and third order derivative Methods in Bulk and Pharmaceutical Dosage Form.@Journal of Chemical and Pharmaceutical Research, 8(4), 272-278.@No$Venkateswarlu K., Venisetty R.K., Yellu N.R., Keshetty S. and Pai M.G. (2007).@Development of HPTLC-UV absorption densitometry method or the analysis of alprazolam and sertraline in combination and its application in the evaluation of the marketed preparation.@Journal of Chromatography, 45(8), 537-543.@Yes
<#LINE#>Consumption pattern and dietary practices of pregnant women during second trimester in Paddipalai divisional Secretariat area of Batticaloa District, Sri Lanka<#LINE#>S. @Amuthenie,R. @Sivakanesan <#LINE#>19-24<#LINE#>4.ISCA-RJRS-2017-025.pdf<#LINE#>Department of Agric. Chemistry, Faculty of Agriculture, Eastern University, Sri Lanka@Department of Biochemistry, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka<#LINE#>14/9/2016<#LINE#>1/1/2017<#LINE#>This study was conducted in antenatal health care clinics at Paddipalai Divisional Secretariat area of Batticaloa District, Sri Lanka during January 2015 to May 2015 to collect data on the eating habit of pregnant women. A total of 55 pregnant women at 12-24 weeks of pregnancy were identified and chosen by random sampling from the seven health centers. Personal interview was conducted privately in the local language (Tamil) with the duration of 20 to 30 minutes. Pre-tested structured questionnaires was used to collect primary data and food frequency questionnaire was used to estimate the frequency of eating different food items. Weight of pregnant women was measured by using solar digital weighing scale of bathroom beam balance scale developed by SECA (Germany). Height was measured in centimeters using a rod attached to the weighing scale and body mass index (BMI) calculated as weight/height2 (kg/m2). “Warrior digital Body Mass Caliper” from Sequoia fitness products USA was used to measure the percentage of Body fat. Collected data was analyzed using SPSS version 19. Results revealed that, 58% of the pregnant mothers were between the age ranges of 20-35. Only 1.8% had university level education while 90.9% had secondary education, 49.1% earn income through labour. On food intake, majority (87.2%) consumed rice on a daily basis. For protein intake, 41.8% consumed fish curry 2-4 times a week while 49.1% consumed chicken curry once in a week. Banana (40%) accounted for the mostly consumed fruit on daily basis. Leafy vegetable (58.2%) are frequently used as 2-4 times per week. Of the milk and milk products, milk is the one that has higher percent (52.7%) of daily consumption, while curd and butter were consumed once in a week by 25.5% and 9.1%, respectively. Low BMI in pregnant women was also recorded in the study area that may leads to low birth weight (LBW) of infants. Therefore, awareness campaign is needed to sensitize the pregnant women on the importance of good nutrition especially fruits, vegetables and dairy products.<#LINE#>Almurshed Khalid S., Bani Ibrahim A., Al-Kanhal Mohammed A. and Al-Amri Mohammed A. (2007).@A study of maternal dietary intake during pregnancy in Riyadh, Saudi Arabia.@Journal of Family and Community Medicine, 14(1), 9-13.@Yes$Metcoff Jack, Costiloe J Paul, Crosby Warren, Bentle Larry, D Seshachalam, Weaver Frances and McClain Philip (1981).@Maternal nutrition and fetal outcome.@American Journal of Clinical Nutrition, 34,708-721.@Yes$Ademuyiwa M.O. and Sanni S.A. (2013).@Consumption Pattern and Dietary Practices of Pregnant Women in Odeda Local Government Area of Ogun State.@Int J Biol Vet Agric Food Eng, 7, 11-15.@Yes$Doerr M. (2001).@Morning sickness, nausea and vomiting during pregnancy: http:// www.storknet.com/complications/ morning sickness/nausea.htm.@Accessed on, 26(8).@Yes$Caplan C.D. (2001).@Evolutionary causes and importance of pregnancy sickness.@http://www.geogle.com/ derekeqplan.htm.@No$Harding J.E. (2001).@The nutritional basis of the fetal origins of adult disease.@International Journal of Epidemiology, 30(1), 15-23.@Yes$Statistical Handbook (2009).@District secretariat@Batticaloa district, Sri Lanka.@No$Nyaruhucha C.N.M. (2009).@Food cravings, aversions and pica among pregnant women in Dar es Salaam, Tanzania.@Tanzania Journal of Health Research, 11(1), 29-34.@Yes$Koryo-Dabrah A., Christina Nti and Adanu R. (2012).@Dietary Practices and Nutrient Intakes of Pregnant Women in Accra, Ghana.@Current Research Journal  of Biological Sciences, 4(4), 358-365.@Yes$Santiago Sarah E., Park Grace H. and Huffman Kelly J. (2013).@Consumption habits of pregnant women and implications for developmental biology: a survey of predominantly Hispanic women in California.@Nutrition Journal, 12(1), 91.@Yes
@Research Article
<#LINE#>Geology and tectonic setting of the volcaniclastic succession of the Eocene, Aliabad area, central Iran<#LINE#>Farzaneh @Rajaie,Mohsen @Ranjbaran,Mojgan Esfahani @Nejad,Azadeh @Rezvani <#LINE#>25-31<#LINE#>5.ISCA-RJRS-2013-978.pdf<#LINE#>Department of Geology, Yerevan University, Yerevan, Armenia@Department of Geology, Faculty of Science, Tehran University, Tehran, Iran@Ideaas Group Inc., Richmond Hill, Ontario, Canada@Department of Geology, Lund University, Lund, Sweden<#LINE#>30/11/2013<#LINE#>26/1/2017<#LINE#>The volcaniclastic rocks of the Eocene are exposed throughout the Aliabad area near the Hoz-e-Soltan lake. The study area is located in central Iran and is a part of Urumieh–Dokhtar volcanic belt. These volcanic rocks generally comprise basic volcanic rocks, volcanic conglomerate and breccias, sandstone, mudstone, and ash beds, deposited by various processes of sediment gravity flows. The article details discussion on the field and laboratory studies in Aliabad, central Iran. The area consists of the Eocene volcanic, volcanosediment, and sedimentary units formed in the marine environments. In thisstudy, we explore the concepts of petrography and sedimentology on the volcaniclastic deposits. The study utilizes modal analysis of sandstones from Aliabad for inferring the sediment provenance in detail.<#LINE#>Pettijohn F.J., Potter P.E. and Siever R. (2012).@Sand and sandstone.@Springer Science & Business Media.@Yes$Zuffa G.G. (1994).@Composition of arenites in sequence stratigraphy.@In H. W. Posamentier.@No$White J.D.L. and Houghton B.F. (2006).@Primary volcaniclastic rocks.@Geology, 34(8), 677-680.@Yes$Johnsson M.J. (1990).@Tectonic versus chemical-weathering control on the composition of fluvial sands in tropical environments.@Sedimentology, 37(4), 713-726.@Yes$Garrels R.M. and Mackenzie F.T. (1971).@Evolution of sedimentary rocks.@A comprehensive account of the origin of sedimentary rocks and their development in space and time. New York: Norton, 397.@Yes$Weltje G.J. and von Eynatten H. (2004).@Quantitative provenance analysis of sediments: Review and outlook.@Journal of Sedimentary Geology, 171(1), 1-11.@Yes$Picard M.D. and McBride E.F. (2007).@Comparison of river and beach sand composition with source rocks. Dolomite Alps drainage basins, northeastern Italy.@In J. Arribas, S. Critelli, & M. J. Johnsson (Eds.), Sedimentary provenance and petrogenesis: Perspectives from petrography and geochemistry. Geological Society of America, Special Papers, 420, 1-12.@Yes$Grantham J.H. and Velbel M.A. (1988).@The influence of climate and topography on rock fragment abundance in modern fluvial sands of the southern Blue Ridge Mountains, North Carolina.@Journal of Sedimentary Petrology, 58(2), 219-227.@Yes$Jones R.L. and Blatt H. (1984).@Mineral dispersal patterns in the Pierre shale.@Journal of Sedimentary Petrology, 54(1), 17-28.@Yes$Jackson M.P.A. (1995).@Retrospective salt tectonics.@Palaios, 18, 1-28.@Yes$Kelley P.H., Kowalewski M. and Hansen T.A. (2003).@Predator–prey interactions in the fossil record.@New York: Kluwer Academic.@Yes$Dickinson W.R. (1970).@Interpreting detrital models of graywacke and Arkose.@Sedimentary Petrology, 40(2), 625-707.@Yes$Dickinson W.R. (1988).@Provenance and sediment in relation to paleotectonics and paleogeography of sediment ary basins.@In K. L. Klein Spehn & C. Paola (Eds.), New perspectives in basin analysis, 3-25.@Yes$Dickinson W.R. and Suczek C.A. (1979).@Plate tectonics and sandstone composition.@Aapg Bulletin, American Association of Petroleum Geologists Bulletin, 63(12), 2164–2182.@Yes$Yerino L.N. and Maynard J.B. (1984).@Petrography of modern marine sands Form the Peru–Chile trench and adjacent areas.@Sedimentology, 31(1), 83-89.@Yes$Dickson J.A.D. (1980).@Staining this section: Alizarin red sand potassium ferricyanide stain.@Unpublished data.@No$Stow D.A.V., Howell D.G. and Nelson C.H. (1983).@Sedimentary, tectonic, and sea level controls on submarine fan and slope-apron turbidite systems.@Geo-Marine Letters, 3(2-4), 57-64.@Yes$Yulong H. and Zhiyong L. (2005).@Interpretation of two-dimensional/three-dimensional seismic data of Aran structural belt in Kashan block.@Tehran: National Iranian Oil Company, 30-40.@No$Folk R.L. and Ward W.C. (1957).@Brazos river bar: A study in the significance of grain size parameters.@Journal of Sedimentary Petrology, 27(1), 3-26.@Yes$Ingersoll R.V., Bullard I.F. and Ford R.L. (1985).@The effect of grain size on detrital modes: A test of the Gazzi–Dickinson point counting method.@Journal of Sedimentary Petrology, 55(4), 618-621.@Yes$Blum M.D., Toomey III, R.S. and Valastro S. (1994).@Fluvial response to late quaternary climatic and environmental change, Edwards Plateau, Texas.@Palaeogeography, Palaeoclimatology, Palaeoecology, 108(1-2), 1-21.@Yes$Berberian M. and King G.C.P. (1981).@Towards a paleogeography and tectonic evolution of Iran.@Canadian Journal of Earth Sciences, 18(2), 210-265.@Yes$Garzanti Eduardo, Canclini Stefania, Foggia Ferdinando Moretti and Petrella Nicola (2002).@Unraveling magmatic and orogenic provenance in modern sand: The back-arc side of the Apennine thrust belt, Italy.@Journal of Sedimentary Research, 72(1), 2-17.@Yes$Shanmugam G. (1985).@Types of porosity in sand stone and their significance in interpreting provenance.@In G. G. Zuffa (Ed.), Provenance of Arenite. Holland: D. Reidal Publishing Company, 115-137.@Yes$Chatroodi A.Y., Karizaki H.S. and Ghorbani M. (2010).@Petrology and geochemistry of volcanic rocks in Hoz-e-Soltan area.@World Academy of Science, Engineering and Technology, 71, 44-48.@No$Amorosi A. and Zuffa G. (2011).@Sand composition changes across key boundaries of siliciclastic and hybrid depositional sequences.@Sedimentary Geology, 236(1), 153-163.@Yes
@Short Communication
<#LINE#>Isolation, identification and biotyping of brucella abortus in cattle and camels in Northern State, Sudan<#LINE#>Zein @A.M. <#LINE#>32-34<#LINE#>6.ISCA-RJRS-2015-055.pdf<#LINE#>Department of Microbiology and Parasitology, Faculty of Medicine and Health Sciences, University of Dongola, Sudan<#LINE#>22/3/2015<#LINE#>19/1/2017<#LINE#>Brucellosis is a thropo-zoonotic infection which can be considered as a great challenge to development of dairy production in developing countries. It is one of the most economically devastating disease, which causes heavy economical losses resulting both from direct effects on animals such as abortions, irregular breeding, sterility and decreased milk yield. From indirect effects on the animal industry associated with costs of veterinary attendance, replacement of animals and impediment to free animal movement and export. The objective of this study was to isolate and characterize Brucella species and determine the biovars associated with the disease. Seven Brucella a bortus biovar l was isolated and identified from cattel and camels in Northern State, Sudan. Tow isolates were isolated from bovine milk. Four from bovine vaginal swabs and one from a placental specimens from a camel. Placental fragment, vaginal swabs, milk and hygroma fluids were collected. Then cultured into Mueller Hinton agar medium. Isolates were identified as Brucella (spp) on the basis of morphology, staining of the organism, production of catalase, oxidase and urease. The colonies appeared after 4-5 days of incubation and were smooth and transparent. Suspected colonies were stained by Gram stain used Modified Zihl-Neelsen (MZN) methods. Gram- negative coccobacilli were found in smears from the colonies. All biochemical tests of the showed characteristics of brucella. The isolates were agglutinated with Brucella A but not with M Brucella antiserum and lyses by Wb, Tb, Bk and Fi phages. We concluded that the seven isolates were sensitive to the antibodies used for treatment of the disease and one isolate show in vitro resistance to Rifampicin.<#LINE#>Luna Martinez J.E and Mejia Teran C. (2002).@Brucellosis in Mexico: Curent Status and trends.@Vet. Microbiol., 90(1), 19-30.@Yes$Clutler S.J, Whatmore A.M, Commander N.J (2005).@Brucellosis, New aspect of an old disease.@J. App Microbiol., 98(6), 1270-1281.@Yes$Bannaty R., Jackson M. and Memuh Z. (1997).@Rapid diagnosis of Brucella Bactermia by using BACTFC 9240 system.@J. Clin Microbiol., 35(10), 2673-2674.@Yes$Majid A.A and Goraish I.A. (1999).@Seroepidemiological observations of camel brucellosis in Eastern and Western Sudan.@Camel Newsletter.,17.@Yes$Makey R.D. (2007).@Modern chemotherapy for brucellosis in human.@Rev.Infect. Dis.,12, 60-99.@Yes$Musa M.T. (1995).@Brucellosis in Darfur States: the magnitude of the problem and methods of diagnosis and control.@PhD Thesis, University of Khartoum, 73-98.@Yes$Alton G.G., Jones L.M., |Angus R.D. and Verger J.M. (1988).@Techniques for the brucellosis laboratory, Paris: Institute National de la Rechereche Agronomique.@@Yes$Musa M.T. and Shigidi M.T.A. (2001).@Brucellosis in camels in intensive animal breeding areas in the Sudan Implication in observations and early life infection.@Rev. Elev. Med . Vet. Day., 54, 11-15.@Yes$Rahman A.A. (2007).@Prevalence of brucellosis in Kuku dairy,Khartoum State and the suceptipility of the isolates to some chemotherapeutic agents.@MSc.hesis, Faculty of Pharmacy, University of Khartoum, Sudan.@Yes$Omer E.E., Habiballa N. and Dafalla E.A. (1977).@Studies on human and bovine brucellosis in the Sudan.@Med.J., 15, 9-16@No
<#LINE#>Phytoplankton population in relation to physico-chemical properties of River Tapi, Surat, Gujarat, India<#LINE#>Taruni @Sarang,Kapila @Manoj <#LINE#>35-37<#LINE#>7.ISCA-RJRS-2017-012.pdf<#LINE#>Dept. of Aquatic Biology, Veer Narmad South Gujarat University, Surat, Gujarat, India@Dept. of Aquatic Biology, Veer Narmad South Gujarat University, Surat, Gujarat, India<#LINE#>30/11/2016<#LINE#>7/2/2017<#LINE#>Monthly variation in Phytoplankton population in relation to Physico-Chemical properties of Tapi river was analysed from September-2015 to February -2016. Water samples and plankton samples were collected from two pre-selected sites. Water samples were analyzed for Temperature, pH, Nitrate, Nitrite, Silicate and Phosphate by using standard methods. Quantitative estimation of Phytoplankton was done by using standard method. Pearson correlation was calculated among the various Physico-Chemical variables and Phytoplankton density.<#LINE#>Fouzia Ishaq, D.R. Khanna and Aamir Khan (2013).@Physico-chemical and phytoplanktonic characteristics of river Tons at Dehradun(Uttarakhand), India.@Journal of Applied and Natural Science, 5(2), 465-474.@Yes$Sharma D.K. and Singh R.P. (2013).@Correlation between Physico-Chemical parameters and Phytoplankton of Tighra reservoir, Gwalior, Madhya Pradesh.@International Journal of security and Networks, 4(1), 90-95.@Yes$Das M. and Panda T. (2010).@Water quality and Phytoplankton population in sewage Fed river of Mahanadi.@Journal of Life scince, 2(2), 81-85.@Yes$Komala H.P., Nanjundaswamy L. and Devi Prasad A.G. (2013).@An assessment of plankton diversity and abundance of Arkavathi River with reference to pollution.@Advance in applied Science Research, 4(2), 320-324.@Yes$Bimbisar D. Waghmare and A.N. Kulkarni (2015).@An Assessment of Phytoplankton Population and Seasonal variation in Lendi River,District Nanded, Maharashtra, India.@Intrnational Journal of Science and Research, 4(12), 936-940.@Yes$Sharma Jyoti, Parashar Alka, Bagre Pratibha and Qayoom Imtiyaz (2015).@Phytoplanktonic diversity and its relation to Physico-Chemical Parameters of water at Dogarwada ghat of River Narmada.@Current world Environment, 10(1), 206-214.@Yes$Sarode  P.T.  and  Kamat  N.D. (1984).@Freshwater diatoms of Maharashtra.@Saikrupa Prakasan.@Yes$Desikachary T.V. (1959).@Cyanophyta.@Indian council of Agriculture Research, New Delhi.@Yes$APHA (2005).@Standard Method for the Examination of Water and Wastewater.@American Public Health Association.@Yes
<#LINE#>Bioremediation of crude oil in synthetic mineral salt medium enriched with bacterial consortium and sapindus mukorossi<#LINE#>Vallimayil @J.,Ramasubramanian @V. <#LINE#>38-39<#LINE#>8.ISCA-RJRS-2017-022.pdf<#LINE#>Department of Botany, The Standard Fireworks Rajaratnam college for Women, Sivakasi,Virudhunagar, Tamilnadu, India@Department of Plant Biology and Plant Biotechnology, Ayya Nadar Janki Ammal College, Sivakasi, Virudhunagar, Tamilnadu, India<#LINE#>30/11/2016<#LINE#>18/1/2017<#LINE#>Soil pollution with crude oil is one of the major environmental problem today. Crude oil is a mixture of hydrocarbons which leads reduction in soil microflora. Hence, a necessary step has to be taken to remove oil pollutants from the soil. Several assays were performed to reduce crude oil contaminated soil in lab scale. Bacterial consortium along with Sapindus mukorossi absorbtion studies was performed. It has improved the remediation process upto 70%.<#LINE#>Usharani K., Sreejina K., Sruthi T. and Vineeth T. (2016).@Diesel Oil Utilization Efficiency of Selective Bacterial Isolates from Automobile Workshop and Thesjaswini River of Kerala.@Pollution Spring, 2(2), 221-232.@Yes$Chang W.N, Liu C.W. and Liu H.S. (2009).@Hydrophobic cell surface and bioflocculation behavior of Rhodococcus erythropolis.@Process Biochem, 44(9), 955-962.@Yes$Dziegielewska E. and Adamczak M. (2013).@Evaluation of waste products in the synthesis of surfactants by yeasts.@Chem. Pap., 67(9), 1113-1122.@Yes$Kasai R., Fujino H., Kuzuki T., Wrong W.H., Goto C., Yata N. et al. (1986).@Acyclic sesquiterpene oligoglycosides from pericarps of Sapindus mukorossi.@Phytochemistry, 25(4), 871-876.@Yes$Makkar R.S., Cameotra S.S. and Banat I.M. (2011).@Advances in utilization of renewable substrates for biosurfactant production.@AMB express, 1(1), 1-5.@Yes$Mukherjee S., Das P. and Sen R. (2006).@Towards commercial production of microbial surfactants.@Trends Biotechnol., 24(11), 509-515.@Yes$Rodrigues L., Banat I.M., Teixeira J. and Oliveira R. (2006).@Biosurfactants: Potential applications in medicine.@J. Antimicrob. Chem., 57(4), 609-618.@Yes$Shavandi M., Mohebali G., Haddadi A., Shakarami H. and Ashrafossadat N. (2011).@Emulsification potential of a newly isolated biosurfactant producing bacterium, Rhodococcus strain TA6.@Collids Surf B, 82(2), 477-482.@Yes$Zi-Jing Tian, Li-Yuan Chen, Da-Hui Li, Hao-Ying Pang, Shuo Wu., Jin-Biao Liu and Lei Huang (2016).@Characterization of a Biosurfactant-producing Strain Rhodococcus sp. HL-6.@Romanian Biotechnological Letters, 21(4), 11650-11659.@Yes