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Antioxidative defense response of selenium by hyper accumulator plant Brassica rapa var. PS66 and Toria towards phytoremediation

Author Affiliations

  • 1 Department of P.G. Studies and Research in Biological Sciences, Rani Durgawati University, Jabalpur, MP, 482001, INDIA

Res. J. Recent Sci., Volume 4, Issue (IYSC-2015), Pages 136-143, September,2 (2015)


Natural processes for instance volcanic eruptions and anthropogenic activities lead to emission of heavy metals in ecosystem. Brassica species have competency to absorb and sequester Se and harness to manage environmental Se contamination via phytoremediation. All the test species of Brassica were found to respond to Se by registering changes in the expression of antioxidative enzymes, and tolerance level showed significant inhibition at higher concentration. The raised value of oxidative stress determinants - lipid peroxidation and hydrogen peroxide, near 100µM signified build up of stress at this concentration. Brassica rapa showed greater Se tolerance as was evident from the increased expression of glutathione peroxidase (GPX) in the treated plants, results corroborated with in gel assays for the enzymes, whereas little or no basal activity was found in the control plants. The positive tie-up between Se enhancement and GPX activity is suggestive for the existence of Se-dependent GPX despite the fact that most plant GPX studied so far have not been reported to require Se for their function. One noteworthy attribute of Brassica rapa PS66 and Toria have adequacy to transform inorganic Se to volatile forms, principally dimethylselenide (DMSe), which is 500-600 times less toxic than the available form of selenium in the environment, thus a prospect benefit for selenium phytoremediation.


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