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Study of Polyhydroxybutyrate producing Bacillussp. isolated from Soil

Author Affiliations

  • 1Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, INDIA
  • 2 Sanghvi Institute of Management and Science, Indore, MP, INDIA

Res. J. Recent Sci., Volume 4, Issue (IYSC-2015), Pages 61-69, September,2 (2015)


Poly-ß-hydroxybutyrate (PHB), one of the polyhydroxyalkanoates is the most popularly used bioplastic. These, being biodegradable and biocompatible polymers, are accumulated as energy reserve granules by many microbia intracellularly under carbon rich and nutrient starving conditions. PHB has proved itself as a promising alternative to non degradable plastics. PHB as bioplastics serves advantage of biological origin and can be completely degraded by variety of microorganisms. In the present study, different PHB producing microorganisms were isolated from soil using E2 medium and its rapid screening for PHB production was performed by Sudan Black B dye plate assay. The PHB accumulators were then subjected to spectrophotometric quantitation method and the highest accumulator was identified and characterized. Isolate KOL IV showed maximum accumulation of PHB and was optimized for its ability to accumulate maximum amount of PHB. It was identified as one of the Bacillus species and could finally accumulate 48% of PHB against dry cell mass after optimization.


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