International Science Community Association Research Journal of Recent Sciences 2277 - 2502 4 ISC-2014 2015 -----Select---- 2 Changes in the histology of Oreochromis niloticus Liver Fed crude extract of Azadirachta indica Saponins 32 36 EN Pravin Dahiphale 1 WE Deppt., CTAE, MPUAT, Udaipur, INDIA P.K.Singh 2 SWE Deppt., CAET, Dapoli, INDIA Kothari Mahesh 3 Kishor Gharde 4 Sagarika Swain 1 Kerala University of Fisheries and Ocean Studies (KUFOS), P.O. Panangad, Kochi, Kerala, INDIA Shyama S. 2 Dinesh K. 3 Sreenath V.R. 4 Adnankhan Golandaj 5 aj 6 Bhojiya A.A. 1 Department of Biotechnology, Mohanlal Sukhadia University, Udaipur-313001, Rajasthan, INDIA Joshi H. 2 P.M. Ingle 1 Department of Irrigation & Drainage Engineering, DBSKKV, Dapoli, MS, INDIA S.E.Shinde 2 AICRP on Water Management, DBSKKV, Dapoli, MS, INDIA M.S. Mane 3 Thokal R.T. 4 Ayare B.L. 5 Sharma Madhu 1 Department of Zoology, Guru Nanak Dev University Amritsar, 143005 Punjab, INDIA Chadha Pooja 2 Obaroh I.O. 1 Department of Biological Sciences, Kebbi State University of Science and Technology Aliero, NIGERIA Keta J.N. 2 Department of Pure and Applied Chemistry, Kebbi State University of Science and Technology Aliero, NIGERIA Elinge M. 3 Department of Biochemistry Kebbi State University of Science and Technology Aliero, NIGERIA Nwaogu J 4 > Research Paper 2014 9 19 2015 -----Select---- 2 Effect of sub-lethal concentrations of crude extract of Azadirachta indica saponins on liver of Oreochromis niloticus was investigated. One hundred and eighty fish were divided into 6 groups (represented by O, A, B, C, D and E) were fed for 56 days with varying sub-lethal concentrations of A. indica saponins (0.5, 1.0, 2.0, 4.0 and 8.0 g/kg with 0.0 g/kg as the control) incorporated into basal diet. The withdrawal effect of A. indica saponins was also investigated after 28 days of feeding all the fish with the control diet. Liver were sectioned into 6 µm using standard procedure for tissue sectioning. Fish fed varying concentrations varying concentrations of A. indica saponins, gradual increase in rodlet cells around the vein, dilation of the veins and thickening of the hepatocytes were observed in the liver, as the concentrations increase from 0.5 to 8.0 g/kg with. Withdrawal effects shows gradual decrease in the rodlet cells, gradual splitting of each vein into two and slight distortion in the wall of the vein were also observed. The changes observed in the vein as a result of withdrawal of A. indica saponins could be processes towards reverting to the normal shape. This study infers that, withdrawal of A. indica saponins may gradually reverse the effects on fish especially at lower concentrations. Copyright@ International Science Community Association