International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Study of phosphate Solubilizing activity of Lead tolerant Pseudomonas aeruginosa HMT 51 isolated from Zawar mines, Udaipur, India

Author Affiliations

  • 1Department of Biotechnology, Vigyan Bhawan, Block B, Mohanlal Sukhadia University, Udaipur, Rajasthan, INDIA

Res. J. Recent Sci., Volume 4, Issue (ISC-2014), Pages 280-282, (2015)

Abstract

Phosphate is one of the most vital macronutrient required for the growth and development of plants. A large number of microorganisms present in the rhizosphere are known to solubilize the insoluble phosphorus present in the soil, converting it into soluble form and make available to the plants. The present study is aimed to determine the efficiency of phosphate solubilizing activity of lead tolerant Pseudomonas aeruginosa HMT51 isolated from mine spoil of Zawar. The phosphate solubilizing activity was determined using modified Sperber medium containing 10% CaCl2 and 10% KHPO by spot assay method. The isolate was found positive as it showed halo zone around its colonies. The efficiency of phosphate solubilization of HMT51 was determined by comparing the diameter of halo zones after 24h, 48h, and 72h. It was found to be 200, 281.81 and 314.28 after 24h, 48h and 72 h respectively. Lead tolerant phosphate solubilizing bacteria will support the plants growing in mine spoil by providing soluble form of phosphate and can thus enhance microbe assisted phytoremediation of metal polluted sites

References

  1. A.H. Goldstein, Bacterial solubilization of mineralphosphates: historical perspectives and future prospects, Am. J. Altern. Agricult, 1, 5765 (1986)
  2. Ivanova R., Bojinova D. and Nedialkova K., Rock phosphate solubilization by soil bacteria, J. of the Univ. of Chem. Technol. And Metal. 41, 297-02 (2006)
  3. Goldstein A.H., Recent progress in understanding the molecular genetics and biochemistry of calcium phosphate solubilization by Gram negative bacteria, Biol. Agric. Hort, 12, 185193 (1995)
  4. Kumar A., Bhargava P., and Rai L.C., Isolation and molecular characterization of phosphate solubilizing Enterobacter and Exiguobacterium species from paddy fields of Eastern Utter Pradesh, India, Afr. J. Microbiol. Res, 4 (9), 820-829 (2010),280-282 (2015)
  5. Vikram A., Alagawadi A.L., Hamzehzarghani H. and Krishnaraj P.U., Factors Related to the Occurrence of Phosphate Solubilizing Bacteria and Their Isolation in Vertisols, Int. J. Res, 2(7), 571-580 (2007)
  6. Foy C.D., Chaney R.L. and White M.C., The physiology of metal toxicity in plants. Annual review of plant physiology, 29, 511-566, (1978)
  7. Shanab R.A., Angle J.S., Delorme T.A., Chaney R.L., van Berkum P., Moawad H., Ghanem K. and Ghozlan H.A., Rhizobacterial effects on nickel extraction from soil and uptake by Alyssum murale, N Phytol., 158(1), 219224 (2003)
  8. Smith S.E. and Read D.J., Mycorrhizal Symbiosis, San Diego: Academic Press Inc, (1997)
  9. Krishnaraj P.U., Genetic characterization of mineral phosphate solubilization in Pseudomonas sp, Ph.D. Thesis, Indian Institute of Agricultural Sciences, New Delhi, India, (1996)
  10. Nguyen C., Yan W., Tacon F.Le. and Lapayrie F., Genetic variability of phosphate solubilising activity by mono-caryotic and dicaryotic mycelia of the ectomycorrhizal fungus Laccariabicolor (Maire) P.D. Orton, Plant and Soil, 143, 193-199 (1992)
  11. Venkateswaran K. and R Natarajan. Seasonal distribution of inorganic phosphate solubilizing bacteria and phosphatase producing bacteria in Porto Novo waters. Indian J. Mar. Sci., 12, 213-217 (1983)
  12. Mishra N., Gupta G. and Jha P.N., Assesment of mineral phosphate-solubilizing properties and molecular characterization of zinc-tolerant bacteria, J. Basic Microbiol., 52, 549-558 (2012)
  13. Pikovskaya R.I., Mobilization of phosphorus in soil in connection with the vital activity of some microbial species, Mikrobiologiya, 17, 362-370, (1948)
  14. Sperber J.I., The incidence of apatite solubilizingorganisms in the rhizosphere and soil, Aust. J. Agri. Res, 9, 778-781 (1958)
  15. Seshadri S., S. Ignacimuthu and C. Laksminarsimhan,Variations in heterotrophic and phosphate solubilizing bacteria from Chennai. South East Coast of India, Indian.J.Mar.Sci., 31, 69-72 (2002)
  16. Shivakumar S., Bhakthavatchalu S. and Shankar B. Sullia., Characterization of multiple plant growth promotion traits of Pseudomonas aeruginosa FP6, apotential stress tolerant biocontrol agent, Annals of Biological Research., 4(2), 214-223 (2013)
  17. Sagervanshi A., P. Kumara, A. Nagee and A. Kumar., Isolation and characterization of phosphate solubilizing bacteria from anand agriculture soil. International journal of life science and pharma research., 2, 256-266 (2012)
  18. Sadaf S. and Ahmed N., Effect of various parameters on the efficiency of zinc phosphate solubilization by indigenous bacterial isolates. African Journal of Biotechnology,7(10), 1543-1549 (2008)
  19. Varsha NHH, Phosphate solubilization by Penicillium spp. closely associated with wheat roots. Biology and Fertility of Soils, 40, 36-43 (2002)
  20. Sandeep C., Radhika R.M., Thejas M.S., Patra S., Gowda T., Suresh C.K. and Mulla S.R., Effect of inoculation of Bacillus megaterium isolates on growth, biomass and nutrient content of Peppermint, J. Phytol, 3(11), 19-24,(2011)
  21. Bolan N.S. and Hedley M.J., Dissolution of phosphate rocks in soils. 2. Effect of pH on the dissolution and plant availability of phosphate rock in soil with pH dependent charge. Nutrient Cycling in Agroecosystems, 24, 125-134 (1990)