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Study of phosphate Solubilizing activity of Lead tolerant Pseudomonas aeruginosa HMT 51 isolated from Zawar mines, Udaipur, India

Author Affiliations

  • 1Department of Biotechnology, Vigyan Bhawan, Block B, Mohanlal Sukhadia University, Udaipur, Rajasthan, INDIA

Res. J. Recent Sci., Volume 4, Issue (ISC-2014), Pages 280-282, (2015)


Phosphate is one of the most vital macronutrient required for the growth and development of plants. A large number of microorganisms present in the rhizosphere are known to solubilize the insoluble phosphorus present in the soil, converting it into soluble form and make available to the plants. The present study is aimed to determine the efficiency of phosphate solubilizing activity of lead tolerant Pseudomonas aeruginosa HMT51 isolated from mine spoil of Zawar. The phosphate solubilizing activity was determined using modified Sperber medium containing 10% CaCl2 and 10% KHPO by spot assay method. The isolate was found positive as it showed halo zone around its colonies. The efficiency of phosphate solubilization of HMT51 was determined by comparing the diameter of halo zones after 24h, 48h, and 72h. It was found to be 200, 281.81 and 314.28 after 24h, 48h and 72 h respectively. Lead tolerant phosphate solubilizing bacteria will support the plants growing in mine spoil by providing soluble form of phosphate and can thus enhance microbe assisted phytoremediation of metal polluted sites


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