International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Identification of Pseudomonas using Probabilistic identification of Bacteria (PIB) Software

Author Affiliations

  • 1Department of Biotechnology, Mohanlal Sukhadia University, Udaipur-313001, Rajasthan, INDIA

Res. J. Recent Sci., Volume 4, Issue (ISC-2014), Pages 14-18, (2015)


The genus Pseudomonas is a metabolically versatile group of gram-negative, motile, rod-shaped bacteria. They are common soil-dwelling aerotactic gram-negative proteobacteria with the unique ability to utilize exotic carbon sources for energy. Some members of the genus Pseudomonas are able to metabolize chemical pollutants in the environment, and as a result can be used for bioremediation. Therefore, characterization of various species of Pseudomonas is of significant importance. In the present study four isolates HMR1, HMR4, HMR7 and HMR16 were characterized on the basis of morphological and biochemical characteristics aided with Probabilistic identification of bacteria (PIB) software. For the isolates, HMR1 and HMR16 identification threshold reached to 1.0 and for the isolates, HMR4 and HMR7 identification threshold reached to 0.98906. Isolate HMR1 and HMR16 were identified as Pseudomonas aeruginosa and isolate HMR4 and HMR7 were identified as Pseudomonas putida.


  1. Shoreit A. and Soltan E., Fluorescent and non-fluorescent Pseudomonas species from Sohag Governorate (Upper Egypt), Bull. Fac. Sci., Assiut Univ., 21, 133-143 (1992)
  2. Soltan E.M., Isolation and Characterization of antibiotic and heavy metal resistant Pseudomonas aeruginosa from different polluted waters in Sohag District, Egypt, Microbiol. Biotechnol., 11, 50-55 (2001)
  3. Hussein H., Farag S., Kandil K. and Moawad H., Tolerance and uptake of heavy metals by Pseudomonads, Process Biochem., 40, 955-961( 2005)
  4. Teitzel G.M. and Parsek M.R., Heavy Metal Resistance of Biofilm and Planktonic Pseudomonas aeruginosa, Appl. Environ. Microbiol., 69, 2313-2320 (2003)
  5. Wang C.L., Michels Schott P.C., Sawson C., Kitisakkul S., Baross J.A., Keasling J.D. and Clark D.S., Cadmium removal by a new strain of Pseudomonas aeruginosa in aerobic culture, Appl. Environ. Microbiol., 63, 4075-4078(1997)
  6. Romling U., Wingender T., Muller H. and Tummler B., A major Pseudomonas aeruginosa clone common to patients and aquatic habitats, Appl. Environ. Microbiol., 60, 1734-1738 (1994)
  7. Palleroni N.J., Pseudomonas classification. A new case history in the taxonomy of gram-negative bacteria, Anton. Leeuw., 64, 231–251 (1993)
  8. Sayler G.S., Hooper S.W., Layton A.C. and King J.M.H., Catabolic plasmids of environmental and ecological significance, Microb. Ecol., 19, 1–20 (1990)
  9. Fredricks D.N. and Relman D.A., Application of polymerase chain reaction to the diagnosis of infectious diseases, Clin. Infect. Dis., 29, 475–478 (1999)
  10. Kwok S. and Higuchi R., Avoiding false positives with PCR, Nature, 339, 237–238 (1989)
  11. Wintzingerode F.V., Gobel U.B. and Stackebrandt E., Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis, FEMS Microbiol. Rev., 21, 213– 229 (1997)
  12. Bryant T.N., PIBWin - software for probabilistic identification, J. Appl. Microbiol.,97, 1326-1327 (2004)
  13. Willcox W., Lapage S., Bascomb S. and Curtis M., Identification of bacteria by computer: theory and programming, J. Gen. Microbiol., 77, 317-330 (1973)
  14. Lapage S.P., Practical Aspects of Probabilistic Identification of Bacteria, Int. J. Syst. Bacteriol., 24, 500-507 (1974)
  15. Joshi H. and Chaudhary B.L., Identification of Lactobacilli by biochemical tests using PIB software, Indian J. Applied and Pure Bio., 18, 179-182 (2003)
  16. Ottaviani D., Masini L. and Bacchiocchi S., A biochemical protocol for the isolation and identification of current species of Vibrio in seafood, J. Appl. Microbiol., 95, 1277–1284 (2003)
  17. Rajput Y., Biswas J. and Rai V., Potentiality Test in Antimicrobial Activity and Antibiotic Sensitivity of Subterranean Streptomyces Strains Isolated from Kotumsar Cave of India, Int. J. Biol. Chem., 6, 1-8 (2012)
  18. Carson J., Wagner T., Wilson T. and Donachie L., Miniaturized tests for computer-assisted identification of motile Aeromonas species with an improved probability matrix, J. Appl. Microbiol.,90, 190-200 (2001)
  19. Bhojiya A.A. and Joshi H.,Isolation and characterization of zinc tolerant bacteria from Zawar Mines Udaipur, India, Int. J. Env. Engg. and Management., 3, 239-242 (2012)
  20. Hugh R. and Leifson E., The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria, J. Bacteriol.,66, 24-26(1953)
  21. Hussein H., Moawad H. and Farag S., Isolation and characterization of Pseudomonas resistant to heavy metals contaminants, Arab J. Biotech., 7, 13-22 (2004)
  22. Tripathi P., Banerjee G., Saxena S., Gupta S.M. and Ramteke P.W., Antibiotic resistance pattern of Pseudomonas aeruginosa isolated from patients of lower respiratory tract infection, African J. Microbiol. Res., 5, 2955-2959 (2011)
  23. Raja C.E., Selvam G.S. and Omine K., Isolation, Identification and characterization of heavy metal resistant bacteria from sewage, International joint symposium on Geodisaster prevention and Geoenvironment in Asia JS-Fukuoka, 205-211 (2009), 14-18 (2015)
  24. Haleem H., Kadhim J., Ilham T. and Banyan A., Isolation of Pseudomonas aeruginosa from Clinical Cases and Environmental Samples, and Analysis of its AntibioticResistant Spectrum at Hilla Teaching Hospital, Med. J. Babylon., 8, 618-624 ( 2011)
  25. Mohamed R.M. and Abo-Amer A.E., Isolation and characterization of heavy-metal resistant microbes from roadside soil and phylloplane, J. Basic Microb., 52, 53–65(2012)
  26. Todar K., Pseudomonas and related bacteria. Todar’s online text book of bacteriology. accessed on 6 April 2006 (2004)
  27. Ceylan Ö. and Uur A., Bio-Monitoring of Heavy Metal Resistance in Pseudomonas and Pseudomonas Related Genus, J. Biol. Environ. Sci.,6, 233-242 (2012)
  28. Dahm H., Wrótniak W., Strzelczyk E., Li C.-Y. and Bednarska E., Diversity of culturable bacteria associated with fruiting bodies of ectomycorrhizal fungi, Phytopathol. Pol.,38, 51–62 (2005)
  29. Gupta M.K. and Singhal P.K., Management practices and bacterial characteristics of hospital wastes in jabalpur (INDIA), J. Environ. Res. Develop., 3, 840-850 ( 2009)
  30. Holmes B., Pinning C.A. and Dawson C.A., A Probability Matrix for the Identification of Gram-negative, Aerobic, Non-fermentative Bacteria that Grow on Nutrient Agar, J. Gen. Microbiol., 132, 1827-1842 (1986)
  31. Badrunnisa S., Shantaram M. and Pai V.R., Isolation, Characterization and identification of bacteria from coolant oils, Int. J. Appl. Biol. Pharma. Tech.,, 444-452 (2011)