International Science Community Association Research Journal of Recent Sciences 2277 - 2502 4 ISC-2014 2015 -----Select---- 2 Antagonistic Effect of Lactobacillus Isolates from cow Milk on selected Pathogenic Bacteria 173 176 EN Pravin Dahiphale 1 WE Deppt., CTAE, MPUAT, Udaipur, INDIA P.K.Singh 2 SWE Deppt., CAET, Dapoli, INDIA Kothari Mahesh 3 Kishor Gharde 4 Sagarika Swain 1 Kerala University of Fisheries and Ocean Studies (KUFOS), P.O. Panangad, Kochi, Kerala, INDIA Shyama S. 2 Dinesh K. 3 Sreenath V.R. 4 Adnankhan Golandaj 5 aj 6 Bhojiya A.A. 1 Department of Biotechnology, Mohanlal Sukhadia University, Udaipur-313001, Rajasthan, INDIA Joshi H. 2 P.M. Ingle 1 Department of Irrigation & Drainage Engineering, DBSKKV, Dapoli, MS, INDIA S.E.Shinde 2 AICRP on Water Management, DBSKKV, Dapoli, MS, INDIA M.S. Mane 3 Thokal R.T. 4 Ayare B.L. 5 Sharma Madhu 1 Department of Zoology, Guru Nanak Dev University Amritsar, 143005 Punjab, INDIA Chadha Pooja 2 Obaroh I.O. 1 Department of Biological Sciences, Kebbi State University of Science and Technology Aliero, NIGERIA Keta J.N. 2 Department of Pure and Applied Chemistry, Kebbi State University of Science and Technology Aliero, NIGERIA Elinge M. 3 Department of Biochemistry Kebbi State University of Science and Technology Aliero, NIGERIA Nwaogu J 4 Adnankhan Golandaj 1 Department of Aquaculture, School of Aquaculture and Biotechnology, KeralaUniversity of Fisheries and Ocean Studies (KUFOS), P.O. Panangad, Kochi, Kerala, INDIA Shyama S. 2 Dinesh K. 3 Sreenath V.R. 4 Sagarika Swain 5 iSumit Nandi 1 Department of Chemistry, Narula Institute of Technology, Agarpara, Kolkata-700109, West Bengal, INDIA Bhattacharyya Rupa 2 Ravi S 1 Department of Electronics, Mangalore University, Mangalagangotri 574199, Karnataka, INDIA Khan A.M. 2 Sure Naveen 1 Department of Civil Engineering, National Institute of Technology, Rourkela-769008, INDIA Patel Asha 2 Jena Biswajit 3 Ghosh ManojKumar 1 Faculty of life science, Department of Biotechnology, AKS University, Satna, MP, INDIA Kumar Samit 2 Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, INDIA Ghosh UttamKumar 3 Chauhan Amit 1 Amity Institute of Forensic Sciences, Amity University, Sec-125, Noida, UP, INDIA Singh Jyoti 2 National Institute of Criminology and Forensic Sciences, Outer ring Road, Rohini-3, INDIA Singh KushwahaK.P. 3 Soni S. 1 The Institute of Science, Mumbai INDIA Andhare V.V. 2 Ingale P.M. 1 Department of Irrigation and Drainage Engineering, DBSKKV, Dapoli, MS INDIA Mohite K.R. 2 B. Tech., (Agril. Engg.) Student, DBSKKV, Dapoli MS, INDIA Mahale D.M. 3 Pawar SandhyaM. 1 Department of Zoology, Padmabhushan Dr. Vasantraodada Patil Mahavidyalaya, Tasgaon, Dist. Sangli, INDIA Basak Pijush 1 Department of Mathematics, Narula Institute of Technology, Agarpara, Kolkata-700109, West Bengal, INDIA Nandi Sumit 2 Department of Chemistry, Narula Institute of Technology, Agarpara, Kolkata-700109, West Bengal, INDIA ChandrashekaraK 1 Department of Physics, St. Philomena College, Puttur -574202, Karnataka, INDIA Karunakara N 2 University Science and Instrumentation Centre (USIC), Mangalore University, Mngalagangotri-574 199, Karnataka, INDIA Somashekarappa H.M. 3 ChavanNilesh S. 1 Department of Zoology, G.E. Society’s, Art’s, Commerce and Science College, Shriwardhan-402 110, Dist.-Raigad, Maharashtra State, INDIA Gharde K.D. 1 SWCE, CTAE, MPUAT, Udaipur, Rajasthan, INDIA Kothari Mahesh 2 SWE, CTAE, MPUAT, Udaipur, Rajasthan, INDIA Mittal H.K. 3 Singh P.K. 4 Dahiphale P.A. 5 Vijayalekshmi V. 1 Dept of Chemistry, S.N College for Women, Kollam, Kerala, 691001, INDIA Ghosh SwapanKumar 1 Department of Jute and Fibre Technology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata-700019, West Bengal, INDIA Jaitly Manjeet 1 PG Department of Zoology, Govt. Dungar College, Bikaner- 334001, Rajasthan, INDIA Khatri AnandKumar 2 Andhare V.V. 1 Poojary B. 2 Rawal I. 1 Department of Biotechnology, Vigyan Bhawan, Block B, Mohanlal Sukhadia University, Udaipur, Rajasthan, INDIA Joshi H. 2 Department of Botany, Vigyan Bhawan, Block B, Mohanlal Sukhadia University, Udaipur, Rajasthan, INDIA Chaudhary B.L. 3 Verma R.K. 1 Deptt. of Medicine, K.G’sMedical University, UP, INDIA Kaur Bhagyapreet 2 Lucknow University, LKO, INDIA Mishra Ravi 3 Karkare A.Y. 1 Rani Laxmibai Girls College, Sawargaon, Dist. Nagpur MS, INDIA Ahirwar J.R. 1 Department of Botany, Govt. P.G. College Niwari, District –Tikamgarh, MP, INDIA Khandelwal D. 1 Department of Biotechnology, Mohanlal Sukhadia University, Udaipur-313001, Rajasthan, INDIA Joshi H. 2 Chaudhary B.L. 3 Chundawat Hemant 1 Joshi Harshada 2 > Research Paper 2014 11 26 2015 -----Select---- 2 The viable microbial food supplements which beneficially influence the health of humans are known as probiotics. Antibacterial activity is one of the important probiotic properties for a strain to be a functional probiotic.The three Lactobacillus strains namely Lactobacillus plantarum CM1, Lactobacillus fermentum CM4 and Lactobacillus casei subsp. casei CM6 were isolated from cow milk samples collected from Udaipur city. Their antagonistic activity was tested against five pathogenic bacteria such as Serratia marcescens NCDC 108, Enterobactor aerogens NCDC 106, Proteus vulgaris NCDC 73, Pseudomonas aeruginosa NCIM 5029, and Micrococcus luteus NCDC 131 using agar well assay method. The results showed that the Lactobacillus strains namely Lactobacillus plantarum CM1, Lactobacillus fermentum CM4 and Lactobacillus casei subsp. casei CM6 were able to inhibit the growth of all the pathogenic bacteria in varying degrees. All the three Lactobacillus isolates were found to be the most sensitive against Micrococcusluteus than the rest of the pathogenic bacteria used in the study. The inhibition observed in the case of all the isolates that have antagonistic effect may be due to the production of organic acids as well as other compounds such as bacteriocins, hydrogen peroxide etc. Copyright@ International Science Community Association