International Science Community Association Research Journal of Recent Sciences 2277 - 2502 4 ISC-2014 2015 -----Select---- 2 Study of Antibiotic Resistance Pattern of Escherichia coli PS 58 Isolated from Pichhola Lake of Udaipur, Rajasthan, India 155 158 EN Pravin Dahiphale 1 WE Deppt., CTAE, MPUAT, Udaipur, INDIA P.K.Singh 2 SWE Deppt., CAET, Dapoli, INDIA Kothari Mahesh 3 Kishor Gharde 4 Sagarika Swain 1 Kerala University of Fisheries and Ocean Studies (KUFOS), P.O. Panangad, Kochi, Kerala, INDIA Shyama S. 2 Dinesh K. 3 Sreenath V.R. 4 Adnankhan Golandaj 5 aj 6 Bhojiya A.A. 1 Department of Biotechnology, Mohanlal Sukhadia University, Udaipur-313001, Rajasthan, INDIA Joshi H. 2 P.M. Ingle 1 Department of Irrigation & Drainage Engineering, DBSKKV, Dapoli, MS, INDIA S.E.Shinde 2 AICRP on Water Management, DBSKKV, Dapoli, MS, INDIA M.S. Mane 3 Thokal R.T. 4 Ayare B.L. 5 Sharma Madhu 1 Department of Zoology, Guru Nanak Dev University Amritsar, 143005 Punjab, INDIA Chadha Pooja 2 Obaroh I.O. 1 Department of Biological Sciences, Kebbi State University of Science and Technology Aliero, NIGERIA Keta J.N. 2 Department of Pure and Applied Chemistry, Kebbi State University of Science and Technology Aliero, NIGERIA Elinge M. 3 Department of Biochemistry Kebbi State University of Science and Technology Aliero, NIGERIA Nwaogu J 4 Adnankhan Golandaj 1 Department of Aquaculture, School of Aquaculture and Biotechnology, KeralaUniversity of Fisheries and Ocean Studies (KUFOS), P.O. Panangad, Kochi, Kerala, INDIA Shyama S. 2 Dinesh K. 3 Sreenath V.R. 4 Sagarika Swain 5 iSumit Nandi 1 Department of Chemistry, Narula Institute of Technology, Agarpara, Kolkata-700109, West Bengal, INDIA Bhattacharyya Rupa 2 Ravi S 1 Department of Electronics, Mangalore University, Mangalagangotri 574199, Karnataka, INDIA Khan A.M. 2 Sure Naveen 1 Department of Civil Engineering, National Institute of Technology, Rourkela-769008, INDIA Patel Asha 2 Jena Biswajit 3 Ghosh ManojKumar 1 Faculty of life science, Department of Biotechnology, AKS University, Satna, MP, INDIA Kumar Samit 2 Department of Polymer and Process Engineering, Indian Institute of Technology Roorkee, INDIA Ghosh UttamKumar 3 Chauhan Amit 1 Amity Institute of Forensic Sciences, Amity University, Sec-125, Noida, UP, INDIA Singh Jyoti 2 National Institute of Criminology and Forensic Sciences, Outer ring Road, Rohini-3, INDIA Singh KushwahaK.P. 3 Soni S. 1 The Institute of Science, Mumbai INDIA Andhare V.V. 2 Ingale P.M. 1 Department of Irrigation and Drainage Engineering, DBSKKV, Dapoli, MS INDIA Mohite K.R. 2 B. Tech., (Agril. Engg.) Student, DBSKKV, Dapoli MS, INDIA Mahale D.M. 3 Pawar SandhyaM. 1 Department of Zoology, Padmabhushan Dr. Vasantraodada Patil Mahavidyalaya, Tasgaon, Dist. Sangli, INDIA Basak Pijush 1 Department of Mathematics, Narula Institute of Technology, Agarpara, Kolkata-700109, West Bengal, INDIA Nandi Sumit 2 Department of Chemistry, Narula Institute of Technology, Agarpara, Kolkata-700109, West Bengal, INDIA ChandrashekaraK 1 Department of Physics, St. Philomena College, Puttur -574202, Karnataka, INDIA Karunakara N 2 University Science and Instrumentation Centre (USIC), Mangalore University, Mngalagangotri-574 199, Karnataka, INDIA Somashekarappa H.M. 3 ChavanNilesh S. 1 Department of Zoology, G.E. Society’s, Art’s, Commerce and Science College, Shriwardhan-402 110, Dist.-Raigad, Maharashtra State, INDIA Gharde K.D. 1 SWCE, CTAE, MPUAT, Udaipur, Rajasthan, INDIA Kothari Mahesh 2 SWE, CTAE, MPUAT, Udaipur, Rajasthan, INDIA Mittal H.K. 3 Singh P.K. 4 Dahiphale P.A. 5 Vijayalekshmi V. 1 Dept of Chemistry, S.N College for Women, Kollam, Kerala, 691001, INDIA Ghosh SwapanKumar 1 Department of Jute and Fibre Technology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata-700019, West Bengal, INDIA Jaitly Manjeet 1 PG Department of Zoology, Govt. Dungar College, Bikaner- 334001, Rajasthan, INDIA Khatri AnandKumar 2 Andhare V.V. 1 Poojary B. 2 Rawal I. 1 Department of Biotechnology, Vigyan Bhawan, Block B, Mohanlal Sukhadia University, Udaipur, Rajasthan, INDIA Joshi H. 2 Department of Botany, Vigyan Bhawan, Block B, Mohanlal Sukhadia University, Udaipur, Rajasthan, INDIA Chaudhary B.L. 3 > Research Paper 2014 11 25 2015 -----Select---- 2 Escherichia coli is the major coliform faecal contaminating bacteria. In the present study an attempt has been made to isolate, identify and detect the antibiotics resistance pattern of E. coli PS 58. Strain PS 58 was isolated on nutrient agar from Pichhola lake of Udaipur and identified on the basis of its morphological and biochemical characteristics. For molecular identification Polymerase Chain Reaction was performed using 16S rRNA gene specific universal primers. 15 commonly used antibiotics i.e. gentamicin, kanamycin, polymyxin, tetracycline, erythromycin, ampicillin, penicillin, amikacin, ciprofloxacin, vancomycin, rifampicin, chloremphenicol, streptomycin, cefixime, trimethoprim were used to detect the antibiotic resistance pattern by disc diffusion method. The results revealed that the strain PS 58 was identified as E. coli (ascession no.KPO99424). It was found strongly resistant to kanamycin, ampicillin, cefixime, polymyxin, penicillin, vancomycin, rifampicin and streptomycin. Copyright@ International Science Community Association