International Science Community Association Research Journal of Recent Sciences 2277 - 2502 4 8 2015 August 2 Comparative study on various Methods for Field Preservation and DNA Extraction of Zoanthids 67 71 EN H. Saleem 1 Department of Management Sciences, Shaheed Zulfikar Ali Bhutto Institute of Science and Technology (SZABIST), Islamabad, PAKISTAN Khan M.A. 2 Alireza Miremadi 1 Sharif University of Technology, International Campus, IRAN EtemadolesllamiBakhtiyari Ali 2 Arash Dorvash 3 Mohammad Aghania 1 Department of management Limkokwing University, Limkokwing Cyberjaya, Malaysia Peyman Attariani 2 Babak Afshar 3 Behnaz Torabi 4 Mohammad Aghaei 1 Department of Business Management, Branch, Tarbiat Moddares University (TMU), Tehran, IRAN Abdolmaleki Sobhan 2 Institute for Management and Planning Studies, IMPS, Tehran, IRAN Amin Asadollahi 3 Department of Business Management, Science and Research Branch, Islamic Azad University, Tehran, IRAN MahinEslami Shahrbabaki 1 Psychiatry Department, Shahid Beheshti hospital, Kerman University of Medical Sciences, Kerman, IRAN Hassan Ziaaddini 2 Institue of Neuropharmachology, Psychiatry Department, Shahid Beheshti hospital, Kerman University of Medical Sciences, Kerman, IRAN Saeedi Hamed 3 Neurology Research Center, Kerman University of Medical Sciences, Kerman, IRAN Khorasani Farshid 4 Neuroscience Research Center, Institue of Neuropharmachology, Kerman University of Medical Sciences, Kerman, IRAN Moghaddam Pourhassan 1 Khorasgan branch, Islamic Azad University, Esfahan, IRAN Mahmud 2 LotfiAhmad Reza 3 Yaghoubi Yaghoub 1 Department of Mechanical Engineering, Khomeinishahr Branch, Islamic Azad University, Khomeinishahr, Isfahan, IRAN Karimian Majid 2 Department of Engineering, Tiran Branch, Islamic Azad University, Tiran, IRAN Kamran Amini 3 Shah RajeshKumar 1 Deptt. of Zoology, D.H.S.K. College, Dibrugarh, Assam, INDIA Das Loveleena 2 Deptt. of Life Sciences, Dibrugarh University, Assam, INDIA Bakhtyarabaslo 1 Islamic Azad University Central Tehran Branch, Faculty of Law, Tehran, IRAN Leila Noroozi 2 MohammadAli Abdolv 1 Department of Business Management, Science and Research Branch, Islamic Azad University (I.A.U), Tehran, IRAN MohammadJavad Taghipourian 2 HarishM.A. Nayaka 1 Department of Sugar Technology, Sir. M. Visvesvaraya Post Graduate Centre, University of Mysore, Mandya, 571 402, Karnataka, INDIA Chandrakanth Vinutha 2 Department of Biochemistry, Adichunchanagiri Biotechnology and Cancer Research Institute, Balagangadharanatha Nagara, 571 448, Nagamangala Taluk, Mandya, Karnataka, INDIA M.S.Lingaiah Prabhu 3 Shivalingaiah Sudarshan 4 Parikh KinjalV. 1 Division of Marine Biology, Department of Zoology, Faculty of Science, The M. S. University of Baroda, Vadodara-390002, Gujarat, INDIA D. ShahPinal 2 Pandya Khushali M. 3 Mankodi PradeepC. 4 > Research Paper 2014 3 3 2015 August 2 Zoanthids, the benthic Anthozoans are found in nearly all coastal marine environments. Zoanthids have become recent interests in the field of scientific study because of the challenge they offer in their taxonomic discernment due to the morphological variability within a species. In these study different methods has been carried out for sample preservation and DNA extraction from Zoanthids. Sample collection and preservation in different fixatives is influential on the quality of DNA during the extraction procedure. The present study is an effort to examine six field fixatives and tries to establish an effective DNA extraction protocol for Anthozoans. Zoanthids were collected and preserved in fixatives like: 1) absolute ethanol, 2) 70% ethanol, 3) lyses buffer with CTAB, 4) Phosphate Buffer Saline with SDS 5) Liquid Nitrogen and 6) Dry Ice. The genomic DNA extraction was carried out using four different protocols, viz. Lyses buffer with proteinase K (LBWPK) 2) Lyses buffer with cetyl trimethyl ammonium bromide (LBWCTAB) 3) Lyses buffer with guanidine Isothiocyanate (LBWGIT) 4) TriZol. The most effective preservative and DNA extraction method, both in term of quality and quantity were confirmed by Gel Analysis and purity was checked by spectrophotometric method. Copyright@ International Science Community Association