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Comparative Study of Antioxidant Capacity of Raw Powder and Waste Black Tea by Frap Assay

Author Affiliations

  • 1Mehsana Urban Institute of Sciences, Ganpat University-384 012, Gujarat, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 42-44, (2014)


Since many centuries tea has been used as important bioactive compounds like folk medicine and it is also an important source of polyphenol as a powerful antioxidant which has used in anticancer activity and protects cell damages from free radicals. This study aimed to compare antioxidant capacity of raw tea powder and tea waste sample by FRAP assay. Antioxidant activity of methanolic (50%) and distilled water extract of raw tea powder and tea waste sample were analyzed by FRAP assay. Total phenolic content was determinedusing Folin-Ciocalteu reagent and calculated as gallic acid equivalent per gram dry weight. Total antioxidant activity was found to be 0.930 mmol FeII/g of dry weight (waste tea) and 0.995 mmol FeII/g of dry weight (raw powder). Total phenolic content was 0.232 g gallic acid/g of dry weight (waste tea) and 0.162 g gallic acid/g of dry weight (raw powder). A correlation between total antioxidant activity, total phenolic content and gallic acid was tested between two samples. Because of contribution of phenolic compounds these samples possess higher antioxidant activity. It is strong radical scavenger and can consider as a good source of natural antioxidant for medicine purposes.


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