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In-vitro evaluation of Antimicrobial potency of commercially available drugs against Dentinal Caries microbes

Author Affiliations

  • 1Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 218-223, (2014)


Bacterial infections are common in dental practice and use of antibiotics for their treatment is also frequent. But their rampant use leads to antimicrobial resistance; a global growing issue affecting both developed and developing countries. Determining the susceptibility profile of potential pathogens is therefore necessary. Efficacy of natural phyto-chemicals isolated from plants and oral hygiene products against Streptococcus mutans; the principal dental pathogen associated with dental caries and other cariogenic bacteria isolated using the method of pure culturing has been evaluated. However, the literature lacks studies evaluating the efficacy of antibiotics against the microflora responsible for dental caries. Therefore, commercially used drugs were screened for their in-vitro antimicrobial potency against the microbial community obtained from dentinal caries lesion using Kirby-Bauer disc diffusion method. The polymicrobial growth showed susceptibility towards all the tested antibiotics except in few samples where Ampicillin/Cloxacillin, Cefixime/Clavulanic acid, Cephalexin, Cefixime and Ampicillin/ Sulbactam showed no zone of inhibition; suggesting possible resistance to such antibiotics in these patients. The comprehensive data obtained may support the polymicrobial etiology of dental caries. Such study may further allow investigation on the spatial distribution of pathogenic, antibiotic resistant bacteria among patients suffering from dental caries.


  1. Costerton J.W., Lewandowski Z., Caldwell D., Korber D. and Lappin-Scott H.M., Microbial biofilms, Annu Rev Microbiol., 49, 711-745 (1995)
  2. Sauer K., The genomics and proteomics of biofilm formation, Genome Biol., 219 (2003)
  3. Bowen W.H., Do we need to be concerned about dental caries in the coming millennium?, Crit Rev Oral Biol Med., 13(2), 126-131 (2002)
  4. Singh V., Chauhan P.K., Choudhary S., Tyagi A. and Kaushal S., AntibioGram pattern of Streptococcus causing dental plaque, International Journal of Universal Pharmacy and Life Sciences., 29-34 (2011)
  5. Smiline G.A., Pandi S.K., Hariprasad P. and Raguraman R., A preliminary study on the screening of emerging drug resistance among the caries pathogen isolated from carious dentine, Indian J Dent Res.,23, 26-30 (2012)
  6. Sato T., Hoshino E., Uematsu H. and Noda T., In vitro antimicrobial susceptibility to combinations of drugs on bacteria from carious and endodontic lesions of human deciduous teeth, Oral Microbiol Immunol., 172-176 (1993)
  7. Oshomoh E.O. and Idu M., Antimicrobial activity of Ethanol and Aqueous extracts of Parinari Curatellifolia (Stem) on dental caries causing microbes, Int J Pharm Sci Res., 2113-2118 (2012)
  8. Prashanth F.M.B., Kannan I., Sambandam C., Jayalakshmi M., Premavathy R.K. and Shantha S., A study on in-vitro antibacterial activity of Ficus bengalensis Linn. on dental caries pathogens Streptococcus mutans and Actinomyces viscosus, Int J Pharm Sci Res., 843-846 (2013)
  9. Lee S.S., Zhang W. and Li Y., The antimicrobial potential of 14 natural herbal dentrifices: Results of an in vitro diffusion method study, J Am Dent Asso.,135, 1133-1141 (2004)
  10. Sentila R., Gandimathi A., Karthika S., Suryalakshmi R. and Michael A., In-vitro evaluation and comparison of the anti-microbial potency of commercially available oral hygiene products against Streptococcus mutans, Indian J Med Sci.,65, 250-259 (2011)
  11. Bauer A.W., Kirby W.M.M., Sherris J.C. and Turck M., Antibiotic susceptibility testing by a standardized single disk method, Am J Clin Pathol., 45, 493-496 (1996)
  12. Marsh P.D., Are dental diseases examples of ecological catastrophes?, Microbiology, 149, 279-294 (2003)
  13. Sharma R., Sharma C.L. and Kapoor B., Antibacterial resistance: current problems and possible solutions, Indian J Med Sci.,59, 120-129 (2005)
  14. Tripathi K.D. Essentials of Medical Pharmacology, 6th ed. Jaypee Brothers Medical Publishers (2008)
  15. Batabyal B., Amoxycillin/clavulanic acid resistance in isolated from infected oral cavity, Indian J.L.Sci., 2(1), 37-40 (2008)
  16. van der Bijl P. and Pitigoi-Aron G., Tetracyclines and calcifiedtissues, Ann Dent., 54(1-2), 69-72 (1995)