International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Synergic Effects of Anticancer Drugs to Bovine Serum Albumin: A Spectroscopic Investigation

Author Affiliations

  • 1School of Chemical Sciences, Mahatma Gandhi University, Kottayam-686560, Kerala, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 157-162, (2014)


Anticancer drugs (i) 5-fluorouracil (5-FU), (ii) azacitidine (AZ) and (iii) cytarabine (CY) (pyramidine analogues) have the ability to quench bovine serum albumin (BSA).The synergic effect between the drugs and BSA were studied using fluorescence spectrophotometer and ultraviolet spectroscopic techniques under imitated physiological conditions. The results indicate that static quenching and non radiative energy transfer are the main reason of fluorescence quenching. The synergism results in both the reduction of the binding stability between drugs and BSA and an increase of the free drug concentration, which will increase the efficacy of drugs. The binding distances (r) between the drugs and BSA were obtained based on Försters theory of non-radiation energy transfer. The results indicated that the effect of synergism affected the conformation of BSA.


  1. Grem J.L., Hoth D.F., Hamilton J.M. and King S.A., The efficacy of 5-fluorouracil in human colorectal cancer is not enhanced by thymidylate synthetase inhibition with CB3717 (N10-propargyl-5, 8 dideazafolic acid), Cancer Treat Rep.,, 71, 1249 -1264 (1981)
  2. Sotos G.A., Grogan L. and Allegra C.J., Paradoxical effects of 5-FU/folinic acid on lymphokine-activated killer (LAK) cell induction in patients with colorectal cancer, Cancer Treat. Rev ., 20, 11- 49 (1994)
  3. Koch-Weser J., Sellers E.M., The effect of age on serum concentrations of albumin and alpha 1-acid glycoprotein, Engl. J. Med., 294, 311-316 (1976)
  4. Bi S., Sun Y., Qiao C. and Zhang H., Spectroscopic and Molecular Docking Investigations on the Interaction of Rutin with Bovine Serum Albumin, J. Lumin.,129, 541- 547 (2009)
  5. He X.M., Carter D.C., Atomic structure and chemistry of human serum albumin, Nature., 358, 209 -215 (1992 )
  6. Lazaro E., Lowe P. J. and Briand X., New approach to measure protein binding based on a parallel artificial membrane assay and human serum albumin, J. Med. Chem., 512, 2009-2017 (2008)
  7. Deepa S., Mishra A. K., Fluorescence spectroscopic study of serum albumin bromadiolone interaction: fluorimetric determination of bromadiolone, J. Pharm. Biomed. Anal, 38, 556 -563 (2005)
  8. Xie M.X., Xu X.Y. and Wang Y.D., Analysis of Binding Interaction between Captopril and Human Serum Albumin, Biochim. Biophys. Acta., 1724, 215–224 (2000)
  9. Li Y, He W.H., Tian J.N. and Tang J.H., The fluorescence spectroscopic study on the interaction between imidazo[2,1-b]thiazole analogues and bovine serum albumin, J. Mol. Struct.,743, 79–84 (2011)
  10. Lemiesz L.T., Interactions between a surface active imidazolium ionic liquid and BSA, Bioorg. Med. Chem., 12, 3269-3275 (2004)
  11. Su\nkowska J., wnick R. and Bojko B., Interaction of anticancer drugs with human and bovine serum albumin, J. Mol. Struct.,133, 651- 653 (2003)
  12. Kandagal P.B., Ashoka S., Seetharamappa, J., Shaikh S.M.T., Jadegoud Y. and Ijare O.B., Study of the interaction of an anticancer drugwith human and bovine serum albumin:Spectroscopic approach, J. Pharm. Biomed., Anal., 41, 393-399 (2006)
  13. Hao H., Ma Q., Huang C. and He F., Ping Y., Preparation, characterization, and in vivo evaluation of doxorubicin loaded BSA nanoparticles with folic acid modified dextran surface, J. Pharm .,28, 77-84 (2013), 157-162 (2014)
  14. Hess M., Jo B.W. and, Wunderlich S., Paclitaxelalbumin interaction in view of molecular engineering of polymer-drug conjugates.Pure Appl. Chem, 81, 439-450 (2009)
  15. Shi Y., Liu H., Xu M., Li Z., Xie G., Huang L. and Zeng Z., Spectroscopic studies on the interaction between an anticancer drug ampelopsin and bovine serum albumin, Spectrochim., Acta Part A., 87, 251-257 (2012)
  16. Yu X., Liu R., Ji D., Yang F., Li X., Xie J., Zhou J. and Yi P., Study on the synergism effect of lomefloxacin and ofloxacin for bovine serum albumin in solution by spectroscopic techniques, J Solution Chem.,40, 521-531 (2011)
  17. Jiang C.Q., Gao M.X. and He J.X., Interaction of human serum albumin with bendroflumethiazide studied by fluorescence spectroscopy, Anal. Chem. Acta., 452, 185-192 (2002)
  18. Hu Y.J., Liu Y., Wang J.B., Xiao, X.H. and Qu S.S., Study of the interaction between monoammonium glycyrrhizinate and bovine serum albumin, J. Pharm. Biomed Anal., 36, 112-132 (2004)
  19. Sklar L.A., Hudson B.S. and Simon R.D., Photochemical dimerization of parinaric acid in lipid bilayers, Biochemistry., 16, 5100-5108 (1997)
  20. Lakowicz J. R., Principles of Fluorescence Spectroscopy, Plenum Publishers New York., (1999)
  21. Kamat B.P., Seetharamap P.J.,Mechanism of interaction of vincristine sulphate and rifampicin with bovine serum albumin: A spectroscopic study, J Chem. Sci., 117, 649–655 (2005)