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Isolation and Characterization of Biofilm Producing Bacteria from Arabian Sea

Author Affiliations

  • 1Dept of Biochemistry and Biotechnology, S.S.V. College, Kerala, INDIA
  • 2Dept of Microbiology, Sree Narayana Guru College, K.G. Chavady, TN, INDIA

Res. J. Recent Sci., Volume 3, Issue (ISC-2013), Pages 132-136, (2014)


Nowadays many studies have been carried out to investigate the occurrence of novel microbial bioactive compounds.Biofilms can produced by microbial species and having fascinating industrial applications. In this present study, water samples from ‘Arabian Sea', isolated organisms were screened for biofilm formation. Potent biofilm producer was identified as Halomonassp(MP) by morphological and biochemical characteristics based on Bergey’s manual of determinative bacteriology, and also by sequencing. Antibacterial activity of EPS from MP was done by disc diffusion method with some pathogenic organisms and characterized. The EPS produced in different environmental factors like incubation temperature 27C and 37C, pH 6 - 8 and in incubation time 24hr, 48hr, 72hr and 96 hr. Maximum EPS production was at pH 8, 27C and at 96 hrs of incubation time. EPS supernatant and Dry EPS produced in each parameters, quantified by total carbohydrate and total protein).


  1. Dalton H.M. and P.E. March, Molecular genetics of bacterial attachment and biofouling, Curr.Opin. Biotechnol,9, 252-255 (1998)
  2. Lee Y.K., Kwon K.K., Cho K.H., Kim H.W., Park J.H. and Lee H.K., Cultureand Identification of Bacteria from Marine Biofilms, J. Microbiol., 183-188 (2003)
  3. Dang H. and C.R. Lovell, Bacterial primary colonization and early succession on surfaces in marine waters as determined by amplified rRNA gene restriction analysis and sequence analysis of 16S rRNA genes, Appl. Environ. Microbiol, 66, 467-475 (1994)
  4. Plante C.J. and Shriver, Differential lysis of sedimentary bacteria by Arenicola marina L.: examination of cell wall structure and exopolymeric capsules as correlates, Jour.Exp. Mar. Biol. Ecol,229, 35-52 (1998)
  5. Simoes M., L.C. Simoes and M.J. Vieira, A review of current and emergent biofilm control strategies, LWT- Food Sci. Technol., 43, 573-583 (2010)
  6. Passow U., Alldredge A.L. and Logan B.E., The role of particulatecarbohydrate exudates in the flocculation of diatom blooms, Limnol. Oceanogr.,41, 335-357 (1994)
  7. Becker A., Katzen F., Puhler A and Ielpi L., Xanthum gum biosynthesis and application: biochemical/genetic perspective, Appl. Microbiol. Biotechnol, 50, 145-152 (1998)
  8. Ohno N., N. Miura, M. Nakajima and T. Yadomae, Antitumor 1-3-glucan from cultured fruit body of Sparasiscrispa, Boil. Pharm. Bul.,23, 866-872 (2000)
  9. Goundy, Determination of extracellular carbohydrate secretion in bacteria, J. Bacteriology (1962)
  10. Lowry S., Determinants of extracellular protein secretion in Gram-negative bacteria, J. Bacteriol.,174, 3423-3430 (1951)
  11. Sinde E. and J. Carballo, Attachment of Salmonella sppandListeriamonocytogenesto stainless steel, rubber and polytetrafluroethylene:the influence of free energy and the effect of commercial sanitizers, Food Microbiol.,17, 439-447 (2000)
  12. Donlan R.M., Role of biofilms in Antimicrobial Resistance, Asaian J.,46, 547-552 (2000)
  13. Djordjevic D., M. Wiedmann and L.A. Mclandsborough, Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation. Appl. Environ. Microbiol.,68(6), 2950-2958 (2002), 132-136 (2014)
  14. Fletcher M. and G.I. Loeb., Influence of substratum characteristics in the attachment of marine pseudomonad to solid surfaces, Appl. Environ. Microbiol.,37(1), 67-72 (1979)
  15. Sommer P., Martin-Rouas C. and Mettler E., Influence of the adherent population level on biofilm population, structure and resistance to chlorination, Food Microbiology, 16, 503–515 (1999)
  16. Lee I.Y., Seo W.T. and Kim G.J., Optimization of fermentation conditions for production of exopolysaccharide by Bacillus polymyxa,Bioprocess Eng.,16, 71-75 (1997)