International Science Community Association Research Journal of Recent Sciences 2277 - 2502 3 9 2014 September 2 Screening of Raw Buffalos Milk from Karnataka for Potential Probiotic Strains 73 78 EN Yasrebdoost Hadi 1 Management Dept, Tabriz Branch, Islamic, Azad University, Tabriz, IRAN SarbazyMoghadam Saeid 2 SalmaGhassem Bagloo 3 Hassanpoor Davood 1 Department of Accounting, Faculty of Management and Accounting, Gilan Science and Research Branch, Islamic Azad University, Gilan, IRAN Pourali MohammadReza 2 Ghatge N.S. 1 Pravara Rural Education Society’s; Home Science and BCA College, Loni. Taluka -Rahata, District-Ahmednagar, Gujarat, INDIA Ashraf Muhammad 1 Department of Computer Science, Federal Urdu University of Arts, Sciences and Technology, Karachi, PAKISTAN Sarim Muhammad 2 Shaikh AbdulBasit 3 Sheikh KashifRaffat 4 Nadeem Adnan 5 Ahsan Kamran 6 Siddiq Muhammad 7 FazlollahZarepour Nasirabadi 1 Department of Public Management, Azad University of Kerman, Kerman, IRAN Vahedi Elham 2 Department of Executive Master of Business Administration,Alborz, IRAN Mahdi Vahedi 3 Department of Industrial Management, Islamic Azad University, Firoozkooh, IRAN Eshaghali Azizi 1 Department of Studies in Education, University of Mysore, Mysore, Karnataka, INDIA Mehri Basnas 1 University of Tabriz (Phd), Bahman Blvd, Tabriz, IRAN Anis Mohd 1 Crystal Growth Laboratory, Department of Physics, Milliya Arts Science and Management Science College, Beed-431122, Maharashtra, INDIA Mariajancyrani 1 Department of chemistry, A.V.V.M. Sri Pushpam College, Poondi, Thanjavur-613 503, Tamil Nadu, INDIA Chandramohan 2 School of Chemical and Biotechnology, Department of Chemistry, SASTRA University, Thanjavur, Tamil Nadu, INDIA Ravikumar 3 Ansari Bahareh 1 Dept. of Agricultural Development, College of Agriculture, Tehran Science and Research Branch, Islamic Azad University, Tehran, IRAN SeyedMehdi Mirdamadi 2 Dept. of Agricultural Extension and Education, College of Agriculture, Tehran Sci. and Research Branch, Islamic Azad Uni., Tehran, IRAN Hosseini SeyedJamalFarajollah 3 Dept. of Renewable Energy and Environmental Engineering, College of New Sciences and Technologies, University of Tehran, Tehran, IRAN Noorollahi Younes 4 G.O. Matthew 1 Glass and Ceramic Technology Department, School of Science and Computer Studies, Federal Polytechnic Ado-Ekiti, Ekiti State, NIGERIA B.O. Fatile 2 Shafakatullah Nannu 1 insNannu Shafakatullah* and M. Chandra Dept. of Bio-Sciences, Mangalore University, Mangalagangothri-574199, Mangalore, Karnataka, INDIA Chandra M. 2 > Research Paper 2014 6 21 2014 September 2 Buffalo’s milk was cultured with appropriate dilution on MRS media for the isolation of potential probiotics and pure cultures were obtained by sub-culturing. Purification of cultures were confirmed by Gram’s staining and catalase test and identified based on morphological, cultural, physiological and different biochemical characteristics as presented in Bergey’s Manual of Systematic Bacteriology. During lactic acid bacterial (LAB) transit through the gastrointestinal tract, ingested microorganisms were exposed to successive stress factors, including low pH in the human stomach and bile salt. These isolates were examined for survival in bile salt, acidic pH, different NaCl concentrations, anti pathogenic activity, as well as survival at different storage temperatures. These stress factors can be used as criteria for the evaluation of probiotic strains. Isolated strains of Lactobacilli spp. and Bifidobacterium spp. showed satisfactory probiotic potentials. Copyright@ International Science Community Association