International Science Community Association Research Journal of Recent Sciences 2277 - 2502 3 12 2014 December 2 Studies for Identification of Polymorphism in Promoter Sequence of growth hormone gene from microminipig (MMP) with direct sequencing PCR 1 8 EN ShedageKishor Ashok 1 Laboratory of Molecular Cell Physiology, Department of Applied Bioresources, Faculty of Agriculture, Ehime University, Matsuyama-790-8566,JAPAN Yasushi Kuwabara 2 Fuji Nojo Service, 5247-34 Kitayama, Fujinomiya, Shizuoka, 418-0012, JAPAN Shunnosuke Abe1 3 Venture Business Laboratory, Ehime University, Matsuyama-790-8577, JAPAN EugeneHayato Morita 4 > Research Paper 2013 11 11 2014 December 2 Growth hormone (GH) is the main protein hormone released from anterior pituitary gland of hypothalamus region of brain in mammals. It is extensively studied in pig and it is encoded by GH gene. Microminipig (MMP) is the world’s smallest pig developed by Fuji Micra Inc, Japan. It weighs 6-7 kg at the age of 6 months. The transcription regulation is the key step in expression of eukaryotic gene expression and it depends on various cis and transacting elements. To our knowledge, the variation in sequence of GH promoter region in MMP, 1 kb upstream from 1st exon has studied first time. All consensus sites are conserved in MMPs except TATA box sequence which has another version such as TATATAAA. The Variations at -934, -713, -626, -574 and -499 sites are observed only in MMPs. These variations of MMPs could be used as possible genetic markers for pig production. Copyright@ International Science Community Association