Research Journal of Recent Sciences _________________________________________________ ISSN 2277-2502 Vol. 2(2), 66-68, February (2013) Res.J.Recent Sci. International Science Congress Association 66 Short Communication Volatile Constituents of Salvia compressa andLogochilus macranthus, two Labiatae Herbs Growing wild in IranFateme Aboee-Mehrizi1*, Mohammad Hossein Farjam, Abdolhossein Rustaiyan, Ali Zare, Maryam SalariDepartment of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, IRAN Department of Chemistry, Firoozabad Branch, Islamic Azad University, Firoozabad, IRANAvailable online at: www.isca.in Received 9th September 2012, revised 22nd November 2012, accepted 20th December 2012Abstract The hydrodistilled volatile oil from aerial parts of Salvia compressa and Logochilus macranthus were analyzed by gas chromatography and mass spectrometry (GC-MS) technique. Eight components of the oil of S.compressa were characterized , representing 97.46% of the total components detected. The major constituent were identified as -Pinen (70.93%), Borneol (7.1%), and Camphen(5.92%). The oil of L.macranthus was characterized by higher amount of caryophyllene oxide (17.52%), Humulene epoxide II (11.02%) and Viridiflorol (9.32%) among the fifteen components comprising 97.46% of the total oil detected. Keywords: Salvia compressa, logochilus macranthus, hydrodistilled volatile. IntroductionThe family umbelliferae is rich in secondary metabolites and embodies numerous genera of high economic and medicinal value, yielding flavonoids, coumarins, acetylenes, terpenes and essential oils 1,2. Genus salvia is one of the largest member of the family lamiaceae, comprising more than 700 species, many of them collected from the wild and a few of them cultivated3,4. Fifty – eight species of the genus Salvia are found in Iran, seventeen of which are endemic. Due to the use of this genus or its essential oils in the food, drug and perfumery industries. Previous chemical investigations on different species of salvia have shown the presences of flavonoids, diterpenoids and even the rare sesterterpenes3-13. The genus lagochilus from lamiacea family consists of forty four species, thirty three of which grow in central Asia. The flora of Iran comprises five species, including four endemics: L. alutaceus Bunge., L.aucheri Boiss., L. kotschyanus Boiss and L. macranthus Fisch . Fc.A.Mey.14,15. In 2009, it was reported that the water distilled oils from the aerial parts of salvia compressa vent. collected from two different localities, were analyzed by Gc and Gc/Ms. The main components found in the oil of S. compressa collected at Tang-e-malavi, was -pinene (18.4%), while in the oil of the plant collected at Mamolan to Pol-e-dokhtar, was, -pinene (4.8%)16. This present study deals with the analysis of the oils isolated from aerial parts of S.compressa collected in province of Bandar Abbas, south of Iran. Material and MethodsPlant Material: The aerial parts of S.compressa and L. macranthus were collected during the flowering stage at the following places. S.compressa was collected from Bandar Abbas, Province of Hormozgan (Iran), In june 2010 and L. macranthus was collected from Nudushan, Province of Yazd (Iran) In April 2011. Voucher specimens have been deposited at the Herbarium of The Research Institute of Forests and Rangelands (TARI), Tehran, Iran. Collection and identification of plant material were greatly supported by Dr.V. Mozafarian (TARI). Extraction of the oils: The oils of the aerial parts of S.compressa and L.macranthus (l00 gr and 80gr, respectively) were obtained by hydro distillation using a Clevenger-type apparatus for 4'h. After decanting, the oils were dried over anhydrous sodium sulfate. They were isolated in Yields of 0.67 % and 0.5% (w/w), respectively. Analysis: GC analysis of the oil from the aerial parts S.compressa and L.macranthus were performed using a Shimadzu 15A gas chromatograph equipped with flame ionization detector (FID) and a DB-5 fused silica column (30 m × 0.25 mm i.d., film thickness 0.25 m). The oven temperature was programmed, 60 ºC (3 min) – 220ºC (5 min) at a rate of 5 °C/min; the injector and detector temperatures were 260°C; the carrier gas was N with a flow rate of 1 ml/min. The sample was injected using the split sampling technique, 1:50. The percentage composition of the oil was calculated automatically from peak areas without any correction. GC-MS analysis was carried out on a Hewlett-Packard 6890/5973 using an HP-5MS column (30 m × 0.25 mm i.d., film thickness 0.25 m). The oven temperature was as above, the transfer line temperature was 260 °C; ionization energy in mass was 70eV; mass range was 40–300 amu; and scan time was 1 s. Retention indices (RI) of compounds were determined relative to the retention times of Research Journal of Recent Sciences ______________________________________________________________ ISSN 2277-2502Vol. 2(2), 66-68, February (2013) Res. J. Recent Sci. International Science Congress Association 67 a series of n-alkanes with linear interpolation. Identification of the oil components was done by comparison of their mass spectra with Chem-station software and Wiley 275 GC-MS library as well as by comparing them with those reported in the literature. The identification of each component was confirmed by comparison of its retention index either with those of authentic compounds or with data in the literature17,18. Results and DiscussionThe composition of the essential oils obtained from the aerial parts of S.compressa , L.macranthus are listed in table 1 and 2. Table-1 Chemical Composition of aerial parts Oils of S.compressaCompound RI a Aerial parts - Pinene 93970.93 Camphene 9535.92 - Pinene 9752.59 - Cymene 10262.92 Limonene 10293.92 Borneol 11697.10 Terpinen-4-ol 1177 2.43 - Eudesmol 1651 4.19 Total % 100 Yield,w/w % 0.67 a kovat's retention index, Tr.: trace (0.05%). Table-2 Chemical Composition of aerial parts Oils of L. macranthus Compound RI a Aerial parts Tricyclene 9264.31 - Terpinene 10172.16 - Caryophyllene 14188.71 Elemene 14372.43 (E) Ionone 14855.88 Caryophyllene oxide 158317.52 Viridiforol 1590 9.32 Humullene epoxide II 1610 11.02 Davanole D1 1616 8.00 -Cadinol 1653 0.2 Khosinol 1680 9.30 1,2-Benzendicarboxylic acid buthyl cyclohexyl ester 1700 6.12 Xanthorrhizol 1753 6.33 2,4,6-Trimethyl benzoic acid 1790 6.12 Total 97.50 Yield,w/w % 0.5 a kovat's retention index, Tr.: trace (0.05%). As it is shown, in the oil of S.compressa, 8 compounds representing 97.46% of total were identified. The oil was rich in monoterpenes (86.28%), including 5 monoterpene hydrocarbons. -Pinen (70.93%), Borneol (7.10%) and Camphene (5.92%) were the major components, in this oil, while -caryophyllene, the major component of the two samples of S.compressa from two different locations, was also found to be the major componenets. As can be seen from the above information, the total amounts of monoterpenes fractions in the oils of S.compressa (86.28%) in work presents,werehigher than the monoterpenes fractions of the oils of S.compressa from two different locations,(23.5%) and 15.5%), respectively. The oil of L.macranthus was characterized by large amounts of oxygenated sesquieterpenes with Caryophyllene oxide (17.52%), Humulene epoxide II (11.02) and Viridilorol(9.32%) being the major constituents found. Conclusion Detailed analysis on chemical composition of essential oil from the S.compressa, showed that this endemic S. compressa species can be exploited as a source of biologically active constituent - Pinene. On the other hand, GC-Mass data of L. macranthus oil’sshowed that this plant can be used as a good source for Caryophyllene oxide, one of the powerful natural antioxidant agents. AcknowledgmentsWe gratefully acknowledge finanicial support from the Research Council of Islamic Azad University of Yazd of Iran and The Science and Research Branch of Tehran, Iran. References1.Gijbels M.J.M., Scheffer J.J.C. and Baerheim Svendsen A., Phthalides in umbelliferae, Rivista Ital.Eppos.,61, 335-341 (1979) 2.Kubeczka K.H., In: Aromatic Plants, Basic and applied aspects. 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