International Science Community Association Research Journal of Recent Sciences 2277 - 2502 2 ISC-2012 2013 February 2 Role of Proteus mirabilis in Caffeine Degradation–A Preliminary Bioinformatics Study 33 40 EN Ahirwar J.R. 1 Department of Botany, Govt. College Niwari, District-Tikamgarh, M.P. INDIA Erhabor J.O. 1 Departement of Plant Biology and Biotechnology, University of Benin, P.M.B 1154, Benin City, NIGERIA Idu M. 2 Benson Idahosa University, PMB, 110, Benin City, NIGERIA Udo F.O. 3 Mehta Kavit 1 Mehsana Urban Institute of Sciences, Ganpat University, Ganpat Vidyanagar, Kherwa, Dist. Mehsana, North Gujarat, INDIA Patel B.N. 2 M.G. Science Institute, Navrangpura, Ahmadabad, INDIA Jain B.K. 3 Mishra Bishwambhar 1 Instrumental and Food Analysis Laboratory, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, INDIA Suneetha Vuppu 2 Surekha M. 1 Toxicology Laboratory, Department of Botany, Kakatiya University, Warangal, Andhra Pradesh, INDIA Kiran S. 2 Naresh A. 3 Reddy S.M. 4 Krishania Suman 1 Department of Life Sciences,The IIS University, SFS Mansarovar, Jaipur -20 (Raj) INDIA Agarwal Kalpana 2 Kelkar TusharS. 1 Environmental Sciences Research Laboratory, Department of Botany, Wilson College, Mumbai, INDIA Bhalerao SatishA. 2 Naina Thangaraj 1 School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, INDIA Sharan Siddharth 2 Vuppu Suneetha 3 > Research Paper 2012 11 19 2013 February 2 An attempt to find the role of Proteus mirabilis in caffeine degradation using bioinformatics tools has been made here. Soils from coffee industries were taken and the bacterium was isolated and found to degrade caffeine. Identification of the bacterium through Sangers dideoxy sequencing of 16S rDNA was done and its genome taken from online database was used for homology modeling of the enzyme to identify regions of similarity and enzyme structure prediction. Also attempts to secondary structure prediction and protein threading has been done to study the enzyme and compare the enzymes of Proteus mirabilis with that of other reported caffeine degrading organisms. Copyright@ International Science Community Association