International Science Community Association Research Journal of Recent Sciences 2277 - 2502 2 ISC-2012 2013 February 2 Genetic variability of Macrophomina phaseolina Affecting Sesame: phenotypic traits, RAPD markers and interaction with the Crop 110 115 EN Ahirwar J.R. 1 Department of Botany, Govt. College Niwari, District-Tikamgarh, M.P. INDIA Erhabor J.O. 1 Departement of Plant Biology and Biotechnology, University of Benin, P.M.B 1154, Benin City, NIGERIA Idu M. 2 Benson Idahosa University, PMB, 110, Benin City, NIGERIA Udo F.O. 3 Mehta Kavit 1 Mehsana Urban Institute of Sciences, Ganpat University, Ganpat Vidyanagar, Kherwa, Dist. Mehsana, North Gujarat, INDIA Patel B.N. 2 M.G. Science Institute, Navrangpura, Ahmadabad, INDIA Jain B.K. 3 Mishra Bishwambhar 1 Instrumental and Food Analysis Laboratory, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, INDIA Suneetha Vuppu 2 Surekha M. 1 Toxicology Laboratory, Department of Botany, Kakatiya University, Warangal, Andhra Pradesh, INDIA Kiran S. 2 Naresh A. 3 Reddy S.M. 4 Krishania Suman 1 Department of Life Sciences,The IIS University, SFS Mansarovar, Jaipur -20 (Raj) INDIA Agarwal Kalpana 2 Kelkar TusharS. 1 Environmental Sciences Research Laboratory, Department of Botany, Wilson College, Mumbai, INDIA Bhalerao SatishA. 2 Naina Thangaraj 1 School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, INDIA Sharan Siddharth 2 Vuppu Suneetha 3 Javed Ahmad 1 Department of biochemical Engineering and Food Technology, Harcourt butler technological Institute, Kanpur, INDIA Khan Iffat 2 Department of Bioscience, Integral University Lucknow, INDIA Ashfaq Ahmad 3 kaushar Imam 4 Dubey S. 1 MVJ College of Engineering, Bangalore, Karnataka, INDIA. Kherwa V. 2 Department of Chemistry, C.D.L.University, Sirsa 125055, Haryana, INDIA. Kherwa V. 1 Department of Chemistry, C.D.L. University, Sirsa 125055, Haryana, INDIA Dubey S. 2 Department of Chemistry, MVJ College of Engineering, Bangalore, Karnataka, INDIA PatelKetan B. 1 Government Science College, Department of Chemistry, Gandhinagar, Gujarat, INDIA Patel YogeshM. 2 Patel RakshaB. 3 Udaykumar P. 1 Department of Chemistry, Birla Institute of Technology and Science, Pilani – K.K. Birla Goa Campus, Zuarinagar, Goa, INDIA Khanna T. 2 Behera R.N. 3 Hassan S.Z. 1 Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, INDIA Chopade S.A. 2 Vinjamur M. 3 LalMohan 1 Department of Chemistry, Jai Narain Vyas University, Jodhpur, Rajasthan, INDIA Gangotri K.M. 2 Sindhu Joseph 1 School of Environmental Studies, Cochin University of Science and Technology, Kochi 682022, INDIA Jyothi K.P. 2 SujaP. Devipriya 3 Suguna Yesodharan 4 Yesodharan E.P. 5 Lakdawala M.M. 1 Chemistry Department, S P T Arts and Science College, Godhra, Gujarat, INDIA Lakdawala J.M. 2 Prime interior, Vapi, Gujarat, INDIA Rojith G. 1 School of Environmental Studies, Cochin University of Science and Technology, Cochin, Kerala, India BrightSingh I.S. 2 Ariponnammal S. 1 Department of Physics, Gandhigram Rural Institute, Deemed University, Gandhigram, Dindigul District, TamilNadu, INDIA Srinivasan T. 2 Ariponnammal S. 1 Department of Physics, Gandhigram Rural Institute, Deemed University, Gandhigram, Dindigul District, Tamil Nadu, INDIA Martínez-Hilders Andrea 1 Departamento de Ciencias Biológicas, Decanato de Agronomía, Universidad Centroccidental Lisandro Alvarado, Barquisimeto, VENEZUELA Mendoza Yuraima 2 Peraza Dasybel 3 Laurentin HernÃn 4 > Research Paper 2012 8 04 2013 February 2 Macrophomina phaseolina is a fungus which affects more than 300 cultivated species. It is one of the most important biotic stresses on sesame (Sesamum indicum L.). A successful control strategy, especially plant resistance management, depends on comprehensive knowledge about genetic variability for both fungus and plant. To evaluate genetic diversity of M. phaseolina affecting sesame in the most important crop production region of Venezuela, seven isolates were characterized by means of phenotypic traits, and RAPD markers. Four of these isolates were used for evaluating the interaction with four sesame genotypes in two ways: interaction in vivo by inoculation, and effect of root and stem extracts on fungus growth. Variability for growth velocity (optical density ranged between 1.69 to 2.32 at 96 hours of growing) (P≤0.05) and microsclerotia production (18-56 in 100 μL) (P≤0.05), was observed. Ten primers used were able to amplify the DNA, generating 81 bands (100% polymorphic). Ordination of the seven isolates by means of principal coordinates analysis based on RAPD did not show a consistent relationship with phenotypic attributes or geographical origin. After inoculation, length lesion produced by the four isolates did not show statistical differences, but germination percentage did (P≤0.01). One of the fungus isolates reduced up to almost 70% average germination of the four sesame genotypes. Mycelial growth of four isolates was inhibited in 17 – 32 % as compared to the control. These results indicate it is difficult to manage charcoal rot by means of obtaining resistant cultivars because of the fungus variability found in all the levels evaluated.. Copyright@ International Science Community Association