International Science Community Association Research Journal of Recent Sciences 2277 - 2502 2 ISC-2012 2013 February 2 Dot Elisa: Immunological Technique for Pesticide Residue Analysis 5 11 EN Ahirwar J.R. 1 Department of Botany, Govt. College Niwari, District-Tikamgarh, M.P. INDIA Erhabor J.O. 1 Departement of Plant Biology and Biotechnology, University of Benin, P.M.B 1154, Benin City, NIGERIA Idu M. 2 Benson Idahosa University, PMB, 110, Benin City, NIGERIA Udo F.O. 3 Mehta Kavit 1 Mehsana Urban Institute of Sciences, Ganpat University, Ganpat Vidyanagar, Kherwa, Dist. Mehsana, North Gujarat, INDIA Patel B.N. 2 M.G. Science Institute, Navrangpura, Ahmadabad, INDIA Jain B.K. 3 Mishra Bishwambhar 1 Instrumental and Food Analysis Laboratory, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, INDIA Suneetha Vuppu 2 Surekha M. 1 Toxicology Laboratory, Department of Botany, Kakatiya University, Warangal, Andhra Pradesh, INDIA Kiran S. 2 Naresh A. 3 Reddy S.M. 4 Krishania Suman 1 Department of Life Sciences,The IIS University, SFS Mansarovar, Jaipur -20 (Raj) INDIA Agarwal Kalpana 2 Kelkar TusharS. 1 Environmental Sciences Research Laboratory, Department of Botany, Wilson College, Mumbai, INDIA Bhalerao SatishA. 2 Naina Thangaraj 1 School of Biosciences and Technology, VIT University, Vellore, Tamil Nadu, INDIA Sharan Siddharth 2 Vuppu Suneetha 3 Javed Ahmad 1 Department of biochemical Engineering and Food Technology, Harcourt butler technological Institute, Kanpur, INDIA Khan Iffat 2 Department of Bioscience, Integral University Lucknow, INDIA Ashfaq Ahmad 3 kaushar Imam 4 Dubey S. 1 MVJ College of Engineering, Bangalore, Karnataka, INDIA. Kherwa V. 2 Department of Chemistry, C.D.L.University, Sirsa 125055, Haryana, INDIA. Kherwa V. 1 Department of Chemistry, C.D.L. University, Sirsa 125055, Haryana, INDIA Dubey S. 2 Department of Chemistry, MVJ College of Engineering, Bangalore, Karnataka, INDIA PatelKetan B. 1 Government Science College, Department of Chemistry, Gandhinagar, Gujarat, INDIA Patel YogeshM. 2 Patel RakshaB. 3 Udaykumar P. 1 Department of Chemistry, Birla Institute of Technology and Science, Pilani – K.K. Birla Goa Campus, Zuarinagar, Goa, INDIA Khanna T. 2 Behera R.N. 3 Hassan S.Z. 1 Department of Chemical Engineering, Indian Institute of Technology Bombay, Mumbai, INDIA Chopade S.A. 2 Vinjamur M. 3 LalMohan 1 Department of Chemistry, Jai Narain Vyas University, Jodhpur, Rajasthan, INDIA Gangotri K.M. 2 Sindhu Joseph 1 School of Environmental Studies, Cochin University of Science and Technology, Kochi 682022, INDIA Jyothi K.P. 2 SujaP. Devipriya 3 Suguna Yesodharan 4 Yesodharan E.P. 5 Lakdawala M.M. 1 Chemistry Department, S P T Arts and Science College, Godhra, Gujarat, INDIA Lakdawala J.M. 2 Prime interior, Vapi, Gujarat, INDIA Rojith G. 1 School of Environmental Studies, Cochin University of Science and Technology, Cochin, Kerala, India BrightSingh I.S. 2 Ariponnammal S. 1 Department of Physics, Gandhigram Rural Institute, Deemed University, Gandhigram, Dindigul District, TamilNadu, INDIA Srinivasan T. 2 Ariponnammal S. 1 Department of Physics, Gandhigram Rural Institute, Deemed University, Gandhigram, Dindigul District, Tamil Nadu, INDIA Martínez-Hilders Andrea 1 Departamento de Ciencias Biol�gicas, Decanato de Agronom�a, Universidad Centroccidental Lisandro Alvarado, Barquisimeto, VENEZUELA Mendoza Yuraima 2 Peraza Dasybel 3 Laurentin Hern�n 4 Shukla Niharika 1 Department of Genetics and Plant Breeding, Jawaharlal Nehru Agricultural University Jabalpur, INDIA Nannu Shafakatullah 1 Department of Bio-Sciences, Mangalore University, Mangalagangothri, Karnataka, INDIA Shetty Sowmyashree 2 OrisonLobo Reema 3 Krishnamoorthy M. 4 Prasad Chandan 1 Dept. of Chemistry, D.A.V College, Kanpur, INDIA Mishra R.P. 2 R and D Division, MRD Life Sciences, Lucknow, INDIA Ali Asif 3 Integral University, Lucknow, INDIA Gangwar V.S. 4 Dept of Chemistry, V.S.S.D College, Kanpur, INDIA Chand Shweta 5 Dept of Chemistry, Christ Church College, Kanpur, INDIA Maru R.N. 1 JJT University, Rajasthan, INDIA Patel R.S. 2 Department of Biology, KKSJ Maninagar Science College, Ahmedabad, Gujarat, INDIA Jyothi K.P. 1 School of Environmental Studies, Cochin University of Science and Technology, Kochi, INDIA Sindhu Joseph 2 Suguna Yesodharan 3 Yesodharan E.P. 4 HridayN. Mishra 1 Physics Department, University of Lucknow, Lucknow, INDIA Srivastava RajeshKumar 2 Narayan Vijay 3 Chand Satish 4 Sachan AlokKumar 5 Shukla VikasKuamr 6 Prasad Onkar 7 Sinha Leena 8 Patel JayKumarShantilal 1 Computer Science Department, Kadi Sarva VishwaVidyalaya, Gandhinagar, Gujarat, INDIA Nakade D.B. 1 Dept. of Microbiology, Rajaram College, Kolhapur, MaharashtraState, INDIA Sreejesh K.K. 1 Kerala Forest Research Institute, Peechi, INDIA Thomas T.P. 2 Rugmini P. 3 Prasanth K.M. 4 Kripa P.K. 5 Prasanth K.M. 1 Dept. of Soil Science, Kerala Forest Research Institute, Peechi, INDIA Sreekala P.P. 2 S. Sandeep 3 Kripa P.K. 4 Sreejesh K.K. 5 Singh R.K. 1 Department of Chemical Engineering, National Institute of Technology, Rourkela, INDIA Biswal Bijayani 2 Kumar Sachin 3 Saravanan V. 1 Department of Tree Breeding, Forest College & Research Institute, Tamil Nadu Agricultural University, Mettupalayam, Tamil Nadu, INDIA Parthiban K.T. 2 Kumar P. 3 Marimuthu P. 4 Kumar P. 1 Department of Tree Breeding, Forest College and Research Institute, Tamil Nadu Agricultural University, Mettupalayam, Tamil Nadu, INDIA Parthiban K.T. 2 Saravanan V. 3 Tiwari Shweta 1 Department of Botany, P.M.B. Gujarati Science College, Indore, INDIA Sharma P.K. 1 Research scholars Zoology, Government P.G. College, Kota, Rajasthan, INDIA Kumar U. 2 Lecturer in Zoology, Government P.G. College, Kota, Rajasthan, INDIA Shrivastava S. 3 RautSonali R. 1 Zoology Dept, Bhavans H. Somani College, Chowpatty, Mumbai-7, INDIA DeshbhratarShantaj M. 2 Botany Dept., Bhavans H. Somani College, Chowpatty, Mumbai-7, INDIA Hile VijayK. 3 Shri Chandulal Nanavati Vinay Mandir, Vile Parle, Mumbai, INDIA Singh AnkitaJ. 4 Tazick Z. 1 Dept. of Plant Protection, Sari Agricultural Sciences & Natural Resources University, Sari , IRAN TajickGhanbary M.A. 2 Dept. of Microbiology, Genetics & Agricultural Biotechnology Institute of Tabarestan, Sari, IRAN Kripa P.K. 1 Dept. of Soil Science, Kerala Forest Research Institute, Peechi, INDIA Prasanth K.M. 2 Sreejesh K.K. 3 Thomas T.P. 4 RamningAmol M. 1 Department of Petroleum Refining and Petrochemical Technology, Laxminarayan Institute of Technology, Nagpur, MH, INDIA Dhote PriyaS. 2 Ganvir V.N. 3 Rai Priyanka 1 School of Environmental biology, A.P.S. University, Rewa MP, INDIA Mishra RM 2 Parihar Sarita 3 Pati Anupama 1 School of Energy and Environmental Studies, Devi Ahilya University, Khandwa Road, Indore, MP, INDIA Chaudhary Rubina 2 Chithra S.V. 1 School of Environmental Studies, Cochin University of Science and Technology, Cochin-22, Kerala, INDIA Amarnath A. 2 S.V. Smitha 3 HarindranathanNair M.V. 4 Amarnath. A. 1 School of Environmental Studies, Cochin University of Science and Technology, Cochin, Kerala, INDIA Rojith. G. 2 Shahin Adhem 3 V.B. Rakesh 4 Ranjita P 1 PG Dept of Environmental Sciences, Sambalpur University, Jyotivihar, Odisha, INDIA P SibaP. 2 Environment Cell, R and D, Hindalco Industries Limited, Hirakud, Sambalpur, Odisha, INDIAI Kar RabiN. 3 nstitute of Minerals $ Materials Technology(CSIR), Bhubaneswar, Odisha, INDIA ChittaR. P 4 Pachauri Tripti 1 Dayalbagh Educational Institute, Dayalbagh, Agra 282 110, INDIA Saraswat RK 2 Singla Vyoma 3 Lakhani Anita 4 KumariMaharaj K. 5 Sharma H.K. 1 M.M University, Mullana, Ambala, INDIA Dogra P. 2 Sharma N. 3 Sharma S. 4 Kumari Om 1 Department of Chemistry, K.K.P. G. College, Etawah, 206001, UP, INDIA Raghav Shailendra 2 Singh Surabhi 3 Srinivas. G 1 Department of Aeronautical and Automobile Engineering, MIT - Manipal University, Manipal, Udupi Karnataka, INDIA PrasadShiva U. 2 M Manikandan 3 Kumar PraveenA. 4 Adhikari Alok 1 Department of Civil Engineering, N.I.T., Rourkela, Odisa, INDIA Patra K.C. 2 Das S.K. 3 Kavuri N.C. 1 Department of Civil Engineering, National Institue of Technology, Roukela 769008, Odisha, INDIA Paul K.K. 2 Roy N. 3 B. Bhuvaneshwari 1 CSIR-Structural Engineering Research Centre, Chennai, INDIA Iyer NageshR. 2 Alka Alka 1 Department of Human Development and Family Studies. College of Home Science, saifabad, Hyderabad, INDIA Mayuri K. 2 SangeetaC. Sindhu 1 Deptt. of Foods and Nutrition, College of Home Science, CCSHAU Hissar, Haryana, INDIA Yadav Saroj 1 Department of Textile and Apparel Designing, I.C. College of Home Science, CCS Haryana Agricultural University, Hisar, INDIA Rose Neelam 2 SinghJeet Saroj 3 Khambra Krishna 4 Ghatge N.S. 1 Pravara Rural Education Society’s, Home Science and BCA College, Loni Taluka Rahata, District, Ahamadnager, INDIA Saraswat Amit 1 Dept. of Mathematics, GLA University Mathura, INDIA Srivastava R.K. 2 Dept. of Mathematics, Agra College Agra, Dr. B.R.Ambedker University Agra, INDIA Banerjee M. 1 Nanoscience and Nanotechnology laboratory, School of Physics, Devi Ahilya University, Vigyan Bhawan, Khandwa Road, Indore, INDIA Sharma A. 2 Shri Dadaji Institute of Technology and Science, Indore Road, Khandwa, INDIA Chongad L. 3 Government Arts and Science College, Ratlam, INDIA Banerjee M. 1 School of Physics, Devi Ahilya University, Vigyan Bhawan, Khandwa Road, Indore, INDIA Chongad L. 2 Government Arts and Science College, Ratlam, INDIA Sharma A. 3 Shri Dadaji Institute of Technology and Science, Indore Road, Khandwa, INDIA Kansal Megha 1 Department of Zoology, Punjab Agricultural University, Ludhiana, Punjab, INDIA SanghaGurinder Kaur 2 Parmar Prabha 1 Department of Zoology, Punjab Agricultural University, Ludhiana, Punjab, INDIA Kocher D.K. 2 > Review Paper 2012 10 01 2013 February 2 Pesticides are used globally for enhancing crop yields. However, their excessive use/misuse, especially in the developing countries, results in widespread food and environmental contamination. Therefore current methods such as gas chromatography and high-performance liquid chromatography have been used successfully, with great sensitivity and reliability, for analysis of many pesticides. However, these classical methods require a high capital expenditure and skilled analysts including time-consuming sample preparation steps. Therefore, there is a growing demand for more rapid and economical methods for determining pesticide residues. Immunoassays have recently been emerging as an alternative to traditional methods to meet such demands of fast, sensitive and cost-effective tool for pesticide residue analysis. The dot ELISA is a qualitative ELISA test, which can be performed more quickly without the need of equipments or technical expertise. Dot ELISA is a micro ELISA, utilizing antigen “dotted” onto nitrocellulose filter discs that has been used for more than 25 years. Because of its relative speed and simplicity, the dot ELISA is an attractive alternative to standard ELISA .This technique can even detect at nano-gram scale among targeted compounds in situ. Copyright@ International Science Community Association