International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

The effect of Nanoliposomal and PE Gylated Nanoliposomal Forms of 6-Gingerol on Breast Cancer Cells

Author Affiliations

  • 1Department of Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, IRAN
  • 2 Pilot Nanobiotechnology Department, Pasteur Institute of Iran, Tehran, IRAN
  • 3 Department of Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, IRAN

Res. J. Recent Sci., Volume 2, Issue (5), Pages 29-33, May,2 (2013)


This study has been devoted to preparing the nanoliposomal and PEGylated nanoliposomal forms of 6-gingerol and investigating their effect on MCF-7 cell line of breast cancer. The formulations of nanoliposomal and PEGylated nanoliposomal forms of 6-gingerol were prepared through reverse-phase evaporation method and using a defined proportion of phosphatidylcholine, cholesterol, 6-gingerol, dextrano-polyethylene glycol 2000 (for PEGylated nanoliposomal form). The encapsulation rate was measured by ultracentrifugation and the drug release rate within 48-hour interval was assessed using dialysis. The MTT assay was used to determine the effect of cytotoxicity rate of formulations on the growth of breast cancer cells, and the viability and IC50 values were calculated for both formulations using the Pharm program. The rate of drug encapsulation was obtained 85% and 92% for nanoliposomal and PEGylated nanoliposomal formulations, respectively. In addition, the diameter and surface potential values were obtained 345.5 nm and -24.5 mV for nanoliposomal formulation, and 310.4 nm and -8.54 mV for PEGylated nanoliposomal form, respectively. The IC50 value for nanoliposomal and PEGylated nanoliposomal forms was obtained to be 11 and 13 g/ml, respectively. Obtained results show that the PEGylated nanoliposomal form of 6-gingerol increases its cytotoxic effect and makes the drug release slower. Therefore, the use of PEGylated nanoliposomal form of 6-gingerol helps efficiently increase its therapeutic index and decrease its side effects in treatment of breast cancer.


  1. Vinay K., Abbas A.L., Fauston N., Robbins and Cotran pathologic basis of disease. 7th Ed., Philadelphia: Elsevier Saunders, (2005)
  2. Kasper D. L., Braunwald E., Fauci A.S., Hauser S.L., Longo D.L., Jameson J. Harrison's principles of internal medicine. 16th Ed., New York, Vol.1 (2005)
  3. Mousavi S.M., Montazeri A., Mohagheghi M.A., Jarrahi A.M., Harirchi I., Breast Cancer in Iran: An Epidemiological Review, The Breast J., 13(4), 383–391 (2007)
  4. Almassi N.F., Akbari H., Madani H., Izadi B., Prevalence of Breast Cancer in Breast Sample Reports in Iran, 2001–2004, The Breast J., 13(5), 536 (2007)
  5. Behjati F., Atri M., Najmabadi H., Nouri K., Zamani M., Prognostic Value of Chromosome 1and 8 Copy Number in Invasive Ductal Breast Carcinoma among Iranian Women: An Interphase FISH Analysis, Pathol. Oncol. Res., 11(3),157-63 (2005)
  6. Azizi F., Hatami H., Janghorbani M., Epidemiology and control prevalent diseases in Iran, Eshtiagh publication, 201-205 (2002)
  7. Whysner J., Wang C.X., Hepatocellular iron accumulation and increased cell proliferation in polychlorinated biphenyl exposed Sprague–Dawley rats and the development of hepatocarcinogenesis, Toxicol. Sci., 62, 36–45 (2001)
  8. Efferth T., Benakis A., Romero M.R., Tomicic M., Rauh R., Steinbach D., Hafer R., Stamminger T., Oesch F., Kaina B., Marschall M., Enhancement of cytotoxicity of artemisinins toward cancer cells by ferrousiron, Free Radic. Biol. Med.,37, 998–1009 (2004)
  9. Singh N.P., Lai H., Artemisinin induces apoptosis in human cancer cells, Anticancer Res., 24, 2277–2280 (2004)
  10. Peng C.A., Ferreira J.F.S., Wood A.J., Direct analysis of Artemisinin from Artemisia annua L. using high-performance liquid chromatography with evaporative light scattering detector, and gas chromatography with flame ionization detector, J Chromatogr A, 1133, 254–258 (2006)
  11. Reungpatthanapong, P., Mankhetkorn, S., Modulation of multidrug resistance by artemisinin, artesunate dihydroartemisinin in K562/adr and GLC/adr resistant cell lines, Biol.Pharmaceut Bulletin, 25, 1555–1561 (2002)
  12. Philips C., and Tsoukas C., Liposomal encapsulation of azidothymidine results in decreased hematopoietic toxicity and enhanced activity against murine acquired immunodeficiency syndrome, Blood,76(5), 1137 - 1143 (1992)
  13. Sharma D., Chelvi T.P., Kaur J., Chakravorty K., De T.K., Maitra A., Ralhan R., Novel Taxol formulation: polyvinylpyrrolidone nanoparticle-encapsulated Taxol for drug delivery in cancer therapy, Oncol Res., 8, 281-286 (1996)
  14. Rosendahl, M., The risk of amenorrhoea after adjuvant chemotherapy for early stage breast cancer is related to inter-individual variations in chemotherapy-induced leukocyte nadir in young patients: data from the randomised SBG 2000-1 study, Eur J Cancer, 45(18), 198-200 (2009)