International Science Community Association Research Journal of Recent Sciences 15 3 2026 07 2 Next generation Molecular tools for tracking Harmful algal bloom and Microbial sources 61 67 EN Sahu Suresh 1 Government Engineering College, Ajmer, Rajasthan, India Kumar Vinod 1 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Aggarwal Akanksha 2 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Kulshershtha Amit 3 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Kumar Manish 4 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Kumar Shanta 5 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Tripathi Raj Kumar 6 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Saini Yashwant Kumar 7 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Gupta Shyam Manohar 8 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Ram Anshu Beulah 1 Department of Biotechnology, Nrupathunga University, Nrupathunga Road, Bangalore, Karnataka 560001, India Kour Gurshish 2 Clinical Microbiology Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu 180 001, India P. Priya 1 Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India P.R. Druthi 2 Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India M. Jeevitha 3 Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India Kabhade Sarina P. 4 Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India Stella Bhupanapadu Sunkesula Mary 5 Insearch Laboratory, Bengaluru, Karnataka, India M. Jeevitha 1 Department of Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India P.R. Druthi 2 Department of Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India P. Priya 3 Department of Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India Kabhade Sarina P 4 Department of Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India Stella Bhupanapadu Sunkesula Mary 5 Insearch Laboratory, Bengaluru, Karnataka, India P. Rajeshwari 1 Government Science College, Chitradurga, Karnataka, India Raveesha K.A. 2 Life Sciences, JSS Academy of Higher Education and Research (JSSAHER) Mysuru, Karnataka, India B. Kavitha 1 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India Shetty Chandana R 2 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India R. Pavithra 3 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India V. Vanaja 4 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India Varshini Vidhya 5 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India N. Anuroopa 1 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India R. Prajwal 2 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India M. Kishore Rao 3 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India C. Deepika 4 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India M. Jayashree 5 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India P. Rashmi 6 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India N. Hithyshi 1 Department of Post-Graduate Studies in Zoology, Nrupathunga University, Bengaluru, India B.C. Shashikanth 2 Department of Post-Graduate Studies in Zoology, Nrupathunga University, Bengaluru, India D.A. Uday Kumar 3 Department of Post-Graduate Studies in Zoology, Nrupathunga University, Bengaluru, India H.M. Mohan Kumar 4 Department of Post-Graduate Studies in Zoology, Nrupathunga University, Bengaluru, India > Research Paper 2026 4 12 2026 07 2 In the recent decade’s escalated anthropogenic pressure and climatic change has led to severe destruction of ecosystem along with the formation of harmful algal blooms. Substantial implications of these harmful algal blooms which constitute one of the major threats to aquatic ecosystem include considerable impact on ecological balance, aquatic biodiversity, human health and blue economy. In order to monitor, detect and mitigate the effects of harmful algal blooms, emphasis has to be given for early detection using molecular tools. In the present study water quality parameters analyzed from different lakes establish a clear link between environmental conditions and quality as well as quantity of living beings present. Highest colony counts reflected on nutrient media were in consistent with the eutrophic conditions. Prevalence of gram negative bacteria, in Lakes B and D were due to their ability to degrade organic matter, contributing to bloom dynamics. Sequencing of purified PCR products from cultured isolates identified dominant genera, Pseudomonas, Vibrio, Aeromonas and Bacillus in Lakes B and D. it is in alignment with the highest read counts in metagenomic analysis. Higher GC content in Lakes B and D is likely due to the dominance of cyanobacteria. Microcystin biosynthesis genes (mcy gene) were most abundant in Lakes B and D correlating with Microcystis abundance. Copyright@ International Science Community Association