International Science Community Association Research Journal of Recent Sciences 15 3 2026 07 2 Production, Optimization and Characterization of L-Glutaminase from Soil-Derived Aspergillus Species under Solid-State Fermentation 55 60 EN Sahu Suresh 1 Government Engineering College, Ajmer, Rajasthan, India Kumar Vinod 1 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Aggarwal Akanksha 2 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Kulshershtha Amit 3 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Kumar Manish 4 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Kumar Shanta 5 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Tripathi Raj Kumar 6 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Saini Yashwant Kumar 7 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Gupta Shyam Manohar 8 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Ram Anshu Beulah 1 Department of Biotechnology, Nrupathunga University, Nrupathunga Road, Bangalore, Karnataka 560001, India Kour Gurshish 2 Clinical Microbiology Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu 180 001, India P. Priya 1 Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India P.R. Druthi 2 Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India M. Jeevitha 3 Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India Kabhade Sarina P. 4 Department of PG studies in Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India Stella Bhupanapadu Sunkesula Mary 5 Insearch Laboratory, Bengaluru, Karnataka, India M. Jeevitha 1 Department of Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India P.R. Druthi 2 Department of Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India P. Priya 3 Department of Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India Kabhade Sarina P 4 Department of Biotechnology, Nrupathunga University, Bengaluru, Karnataka, India Stella Bhupanapadu Sunkesula Mary 5 Insearch Laboratory, Bengaluru, Karnataka, India P. Rajeshwari 1 Government Science College, Chitradurga, Karnataka, India Raveesha K.A. 2 Life Sciences, JSS Academy of Higher Education and Research (JSSAHER) Mysuru, Karnataka, India B. Kavitha 1 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India Shetty Chandana R 2 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India R. Pavithra 3 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India V. Vanaja 4 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India Varshini Vidhya 5 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India N. Anuroopa 1 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India R. Prajwal 2 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India M. Kishore Rao 3 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India C. Deepika 4 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India M. Jayashree 5 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India P. Rashmi 6 Department of Microbiology, Nrupathunga University, Bengaluru, Karnataka, India > Research Paper 2026 4 11 2026 07 2 L-glutaminase (EC 3.5.1.2) is an amidohydrolase enzyme of significant biomedical and industrial importance due to its potential therapeutic application in cancer treatment and its role in food biotechnology. The present study aimed to isolate, produce, purify, and characterize L-glutaminase from Aspergillus species obtained from soil samples. Fungal isolates were screened using a modified Czapek’s medium supplemented with L-glutamine and Congo red dye indicator. Positive isolates were further confirmed by thin layer chromatography for enzymatic activity. Quantitative estimation of enzyme activity was carried out using Nessler’s reagent by measuring ammonia released from L-glutamine hydrolysis. Optimization studies were performed to evaluate the influence of physicochemical parameters such as pH, temperature, moisture content, inoculum size, and incubation period on enzyme production. Among the screened isolates, strain S3 showed the highest enzyme activity and was identified as Aspergillus species through microscopic examination. Maximum enzyme activity of 15.4 µmol/ml/min was observed at pH 5.0, 30°C, and 60% moisture content under solid-state fermentation. The enzyme exhibited high specificity toward L-glutamine and demonstrated stability across a range of environmental conditions. The results indicate that Aspergillus species can serve as a promising microbial source for L-glutaminase production with potential applications in oncology, biosensor development, and food fermentation industries. Copyright@ International Science Community Association