International Science Community Association Research Journal of Recent Sciences 15 3 2026 07 2 Efflux Pumps of Mycobacterium tuberculosis: Expression analysis and in-silico characterization 16 28 EN Sahu Suresh 1 Government Engineering College, Ajmer, Rajasthan, India Kumar Vinod 1 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Aggarwal Akanksha 2 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Kulshershtha Amit 3 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Kumar Manish 4 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Kumar Shanta 5 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Tripathi Raj Kumar 6 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Saini Yashwant Kumar 7 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Gupta Shyam Manohar 8 NTPC Energy Technology Research Alliance (NETRA), Plot No. E-3, Ecotech-II, Udyog Vihar, Greater Noida -201306, Uttar Pradesh, India Ram Anshu Beulah 1 Department of Biotechnology, Nrupathunga University, Nrupathunga Road, Bangalore, Karnataka 560001, India Kour Gurshish 2 Clinical Microbiology Division, Indian Institute of Integrative Medicine (CSIR), Canal Road, Jammu 180 001, India > Research Paper 2026 4 10 2026 07 2 Multidrug resistant tuberculosis, a global threat to the world community, involves high cost and long duration of treatment. Emergence of drug resistance is attributed to activity of efflux pumps as one of the earliest cause in mycobacterium. Here we show the real time expression profiles of 20 probable efflux pumps of Mycobacterium tuberculosis under the two most important drugs of antiTB regime – rifampin and isoniazid. Under rifampin pressure 10 efflux pump genes were over expressed (Rv1258c, Rv1410c, Rv1819c, Rv1145, Rv2936, Rv2937, Rv0849, Rv1877, Rv2209, and Rv0783). Under isoniazid pressure 4 efflux pump genes were induced (Rv1258c, Rv1819c, Rv2938, and Rv3065). The three dimensional structure of Rv1410c was modelled that shares significant structural similarity with MFS transporter proteins. Docking with rifampin revealed the drug’s binding pattern. It is hoped that current study will provide significant information regarding efflux mediated drug resistance of mycobacterium and can facilitate the development of new potent efflux pump inhibitors against tuberculosis. Copyright@ International Science Community Association