International Science Community Association Research Journal of Recent Sciences 15 2 2026 04 2 Formulation of Soaps by mixing varying proportions of Non-Edible Oils and investigation of their Antimicrobial activity on different microbes 15 21 EN Bhandari Jatin 1 Biology Department, B.K.M. Science College, Valsad, 396001, Gujarat, India and Department of Biosciences, Veer Narmad, South Gujarat University, Surat, 395007, Gujarat, India Gohil Takhatsinh 2 Biology Department, B.K.M. Science College, Valsad, 396001, Gujarat, India Yadav Sarita 1 Department of Chemistry, Ram Jaipal Collage Chapra, Jai Prakash University, Chapra, Saran, Bihar -841301, India Yadav Sudha 2 Department of Chemistry, DDU Gorakhpur University Gorakhpur-273009, Uttar Pradesh, India Rangwala Juzer Ali 1 Department of Chemistry, Govt. Holkar Science College, Indore, M.P., India Sarasan Geetha 2 Department of Chemistry, Govt. Holkar Science College, Indore, M.P., India > Research Paper 2026 1 18 2026 04 2 In this investigation, different samples of soap were prepared by mixing non-edible Jatropha Oil with non-refined Cottonseed oil in varying proportions. Spectroscopic Technique like Infrared spectroscopy method was used to investigate the molecular structure of the synthesized product. Different parameters related to the quality evaluation of soap, such as soap yield, moisture content (%), pH, foam ability, etc., were determined using standard protocols. The alkalinity of Soap samples was expressed in terms of Phenolphthalein alkalinity, and Total Alkalinity (in terms of % of carbonate) was determined using the pH meter titration method. To study the medicinal efficacy of the derived product, three different microbes, Staphylococcus aureus, Salmonella enterica and Candida albicans were chosen as test organisms, and the antimicrobial activity of soap solutions at different dilutions was evaluated by the Muller-Hinton agar Plate method. Soaps made from non-edible oils have good efficacy, and these soaps can be used to produce cosmetic formulations, household cleansing agents and antibacterial/antifungal solutions valuable in the pharmaceutical sector. Copyright@ International Science Community Association