Research Journal of Recent Sciences _________________________________________________ ISSN 2277-2502
Vol. 1 (ISC-2011), 361-364 (2012)
Res.J.Recent Sci.

Short communication

Evaluation of one Rapid Method for Diagnosis of Malaria
The Optional and Better Replacement of Microscopy
Panchal Hetal K.1 and Desai Pratibha B.2
1

Dolat Usha Institute of Applied Sciences, Valsad; Veer Narmad South Gujarat University, Surat, Gujarat, INDIA
2
Shree Ram Krishna Institute of Comp. Edu. and Applied Sciences; Veer Narmad South Gujarat University, Surat, Gujarat, INDIA

Available online at, www.isca.in
(Received 27th September 2011, revised 16th January 2012, accepted 28th January 2012)

Abstract
Microscopy has been the most trustable technique for the diagnosis of malaria in India. Reduction of morbidity and mortality rate
of malaria highly influenced by earlier and proper diagnosis. This study was carried out at Valsad, Gujarat. It involved use of
microscopy i.e. field’s stain and detection of Plasmodium falciparum - HRP II antigen, Plasmodium vivax - pLDH antigen detection
by one rapid diagnostic Test (RDT) SD Bioline. Present study was carried out from 966 EDTA anticoagulated samples collected
from clinical laboratories and hospitals of Valsad. Microscopic examinations of stained thick and thin films, shows 8.39%, 13.97%,
0.21% were detected as Plasmodium falciparum, P. vivax and mix respectively. Whereas with Rapid Diagnostic test using HRP II,
p-LDH antigens 9.05% and 13.87% were detected as P. falciparum, P. vivax respectively. The study shows reasonable harmony
between microscopy and RDT. Among two methods RDT was found to have high sensitivity (97.70%) and specificity (98.93%)
compared to microscopy. Though the microscopic method is cost effective but laborious and needs an expertise. The RDT results
were highly accurate and can be used where microscopy is inaccurate or in case of unavailability of expert.
Keywords: Malaria, malaria diagnosis, rdt, microscopy.

References
1. http://www.flutrackers.com/forum/showthread.php?t=1
70884
2. Lim H.S., and Kim H. S.,Evaluation of diagnostic
methods of re-emerging malaria in Korean patients,
Yonsei Medical Journal, 42(1), 84-90, (2001)
3. Parajuli K., Hanchana S., Imwong M., Pukrittaya
kayamee S., and Ghimire P., Comparative evaluation of
microscopy and polymerase chain reaction for the
diagnosis in suspected malaria patients of Nepal, Nepal
Med Coll J, 11(1), 23-27, (2009)
4. Srivastava H.C., and Yadav R.S., Malaria outbreaks in
tribal area of Gujarat state, India, (2000)
5. WHO/CDS/RBM, New perspectives malaria diagnosis,
Report of a joint WHO/USAID informal consultation,
WHO, Geneva, (2000)
6. Rougemont M., Saanen M.V., and sahli R. et al.,
Detection of four Plasmodium species in blood from
humans by 18S rRNA gene subunit-based and speciesspecific
real-time PCR assays, J Clin Microbiol, 42,
5636-43, (2004)
7. Makler M.T., palmer C.J., and Ager A.L., A review of
practical techniques for the diagnosis of malaria, Ann
Trop Med Parasitol, 92, 419-33, (1998)
8. Basco L.K., Marquet F., Makler M.M., and Bras J.L.,
Plasmodium falciparum and Plasmodium vivax, Lactate
Dehydrogenaste Activity and its Application for in vitro
drug Susceptibility Assay, Experimental Parasitology,
80, 260-271, (1995)
9. Histidine-Rich Protein II, a Novel Approach to Malaria
Drug Sensitivity Testing ANTIMICROBIAL AGENTS
AND CHEMOTHERAPY”, 46(6), 1658-1664, (2002)
10. Peyron F., Martet G., and Vigier J.P., Dipstick antigen
capture assay for malaria detection, Lancet, 343(8911),
1502-1503, (1994)
11. Singh N., Valecha M., and Sharma V. P., Malaria
diagnosis by field workers using an immune
chromatographic test, Transactions of the Royal Society
of Tropical Medicine and Hygiene, 91(4), 396-397,
(1997)
12. World Health Organization, Manual of basic techniques
for a health laboratory, 2nd edition, (2005)
13. World Health Organization, Management of
uncomplicated malaria and the use of antimalarial drugs
for the protection of travellers, Information consultation
report, Geneva, WHO/MAL, 1075-98, (1995)
14. Warhurst D.C., and Williams J.E., Laboratory diagnosis
of malaria, ACP broadsheet no. 148, J. Clin. Pathol, 49,
533-538, (1996)
15. Humar A., Ohrt C., Harringhton M.A., Pillai D., and
Kain C.A., ParaSight-F test compared with the
polymerase chain reaction and microscopy for the
diagnosis of Plasmodium falciparum malaria in
travelers, Am J Trop Med Hyg, 56, 44-48, (1997)
16. Uguen C., Rabodonirina M., De Pina J.J., Vigier v,
Martet G., Maret M., and Peyron F., ParaSight - F rapid
manual diagnostic test of Plasmodium falciparum
infection, Bull WHO, 73, 643-649, (1995)
17. Laferi H., Kandel K., and pichler H., False positive
dipstick test for malaria, N Engl J Med, 337, 1635-
1636, (1997)
18. Baetoloni A., Strihmeyer M., Sabatinelli G., benucci
M., Serni U. and Paradisi F., False positive ParaSight-F
test for malaria in patients with rheumatoid factor,
Trans R Soc Trop Med Hyg, 92, 33-34, (1998)
19. Mishra B., Samantaray J.C., Kumar A., and Mirdha B.
R., Study of false positivity of two rapid antigen
detection tests for diagnosis of Plasmodium falciparum
malaria, J Clin Microbiol, 37, 1233, (1999)
20. Ndao M., Bandyayera E., and Kokoskin E. et al.,
Comparison of blood smear, antigen detection, and
nested-PCR methods for screening refugees from
regions where malaria is endemic after a malaria
outbreak in Quebec, Canada. J Clin Microbiol, 42,
2694-700, (2004)