Research Journal of Recent Sciences ________________________________________________ ISSN 2277 - 2502
Vol. 1(ISC-2011), 345-347 (2012)
Res.J.Recent.Sci.

Short Communication

A Calorimetric Study on the Interaction between Vitamin-B6 and lysozyme
Barzegar L.1, Rezaei Behbehani G.1, Moosavi M.2 and Mehreshtiagh M.3
1

Chemistry Department, Azad Takestan University, Qazvin, IRAN
2
Chemistry Department, Payam Noor University, Abhar, IRAN
3
Chemistry Department, Imam Khomeini International University, Qazvin, IRAN

Available online at: www.isca.in
(Received 30th September 2011, revised 20th January 2012 , accepted 21th February 2012)

Abstract
The binding reaction between vitamin B6 (B6, pyridoxine) and lysozyme (Lys) was investigated for the first time by isothermal
titration calorimetry (ITC), at pH 7 at 27°C in tris buffer (25mmol.L-1). The enthalpies of LYS+B6 interaction are reported and
analysed in term of the extended solvation model. The thermodynamic parameters, enthalpy changes (∆H) and entropy changes
(∆S) were calculated. These data suggested that hydrophobic interaction was the predominant intermolecular forces stabilizing the
complex, which was in good agreement with the results of molecular modeling study. It was found that LYS has one noncooperative binding site for Vitamin B6.
Keywords: Vitamin B6, lysozyme, isothermal titration calorimetry.

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