International Science Community Association Research Journal of Recent Sciences 2277 - 2502 1 3 2012 March 2 Synthesis of methyl cinnamate using immobilized lipase from B. licheniformis MTCC-10498 68 71 EN Chauhan RekhaRani 1 Department of Botany, KGK PG College, Moradabad-244001, UP, INDIA Chaudhary Reema 2 Plant Physiology Laboratory, Department of Botany, Hindu College Moradabad-244001, UP, INDIA Singh Alka 3 Singh P.K. 4 Srivastava RajeshKumar 1 Physics Department, University of Lucknow, Lucknow, INDIA Narayan Vijay 2 Kumar Amarendra 3 Prasad Onkar 4 Sinha Leena 5 Bayani A.S. 1 Indian Institute of Science Education and Research, Pune, MS, INDIA Trivedi J.N. 2 Division of Environment and Toxicology, Dept. of Zoology, The Maharaja Sayajirao University of Baroda, Vadodara, INDIA Sharma R.L. 1 Hi-tech laboratory of Molecular developmental Biology, Department of Zoology, M.D.S. University, Ajmer, Rajasthan, INDIA Sharma K.K. 2 Sharma N. 3 MangaleSapana M. 1 Department of Environmental Science, Shivaji University, Kolhapur, INDIA Chonde SonalG. 2 Raut P.D. 3 SrideviV. Dhanalakshmi 1 Department of Chemistry, GKM College of Engineering and Technology, Chennai – 63, TN, INDIA Ramanujam R.A. 2 Environment Technology Division, (CLRI), Council of Scientific and Industrial Research (CSIR), Adyar, Chennai, INDIA Deliana Yosini 1 Faculty of Agriculture Padjajaran University, Bandung, INDONESIA Ananadhi PadmanabhanM.R. 1 Department of Biotechnology, Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai – 119, Tamil nadu, INDIA Stanley ShaleeshaA. 2 Department of Biotechnology, Jeppiaar Engineering College, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai–119, Tamil Nadu, INDIA Deshpand D.P. 1 Dept. of Chemical Engg. Jawaharlal Darda Institute of Engg. and Tech Yavatmal, Maharashtra, INDIA Warfade V.V. 2 Amaley S.H. 3 d Lokhande D.D. 4 Sharma C.K. 1 Department of Biotechnology, Himachal Pradesh University, Shimla-171005, HP, INDIA Kanwar S.S. 2 > Short Communication 2012 1 4 2012 March 2 Methyl cinnamate was synthesized by the reaction of methyl alcohol with cinnamic acid using silica immobilizedlicheniformis MTCC-10498. The silica-bound lipase was used to perform esterification of methanol with cinnamic acid in equimolar ratio (100 mM each) in DMSO. Moreover, pure methyl cinnamate was used as internal standard. Methyl cinnamate was synthesized by manipulating various parameter i.e. relative proportions of reactants, reaction temperature, reaction timeconcentration of molecular sieves and salt ions. Finally, 64.3 mM methyl cinnamate was synthesized at 65each of the reactants in 12 h in the presence of molecular sieves (100 mg/reaction volume) under shaking at 120 rpm. The immobilized lipase synthesized 50 % ester u Copyright@ International Science Community Association