International Science Community Association Research Journal of Recent Sciences 2277 - 2502 1 10 2012 October 2 Quantitative Determination of Selenium and Development of Chemical Sensing Indicator Plates via New Synthesized Dye and its Application in Water and Plant Samples 51 54 EN Espinoza E. 1 Instituto de Investigaciones Metalurgicas, Universidad Michoacana de San Nicolas de Hidalgo, Ciudad Universitaria Edificio ”U”, Apartado Escudero R. 2 postal 888, C.P. 58000, Morelia, Michoacán, MÉXICO Tavera F.J. 3 PranavC. Atre 1 School of Mechanical and Building Sciences, VIT University, Vellore-632014, Tamilnadu, INDIA ThundilKaruppaRaj R. 2 Mehrdad Zarenejad 1 Department of Economic, Qaemshahr Branch, Islamic Azad University, Qaemshahr, IRAN Rodrigues L.L.R. 1 Department of Mechanical and Manufacturing Engineering, Manipal Institute of Technology, Manipal 576 104, Karnataka, INDIA Kantharaj A.N. 2 Department of Automobile and Aeronautical Engineering, Manipal Institute of Technology, Manipal 576 104, Karnataka, INDIA Kantharaj B. 3 Freitas W.R.C. 4 Murthy B.R.N. 5 Serban C. 1 Faculty of Marketing, The Bucharest University of Economic Studies, Bucharest, ROMANIA Iconaru C. 2 Macovei O.I. 3 Perju A. 4 Kumar Anil 1 Department of Physics, P.L.J.L. Rastogi Inter College, Moradabad 244 001, Uttar Pradesh, INDIA ChauhanRekhaRan RekhaRani 2 Department of Botany, K.G.K. College, Moradabad-244001, Uttar Pradesh, INDIA Kumar Pradeep 3 Department of Physics, K G K College, Moradabad 244001, Uttar Pradesh, INDIA S. Anbarasu 1 Department of Physics, St. Xavier’s College (Autonomous), Palayamkottai – 627002, INDIA DevarajanPrem Anand 2 SheikhKashif Raffat 1 Department of CS and IT, Federal Urdu University of Arts, Sciences and Technology, Karachi, PAKISTAN Mohd.Shahab Siddiqui 2 HIIT, FEST, Hamdard University, Karachi, PAKISTAN Mohd. Siddiq 3 National University of Computer and Emerging Sciences, Karachi, PAKISTAN Shaikh ZubairA. 4 FEST, Hamdard University, Karachi, PAKISTAN AbdulRahman Memon 5 Dubey SharmaRuchi 1 Department of Chemistry BUIT, Barkatullah University Bhopal, MP, INDIA Joshi Smita 2 Department of Chemistry, Sarojini Naidu Govt. Girls PG College, Bhopal, MP, INDIA Amlathe Sulbha 3 Department of Chemistry BUIT, Barkatullah University, Bhopal, MP, INDIA > Research Paper 2012 7 27 2012 October 2 A selective, simple, inexpensive and a new reagent for determination of selenium is proposed. This method is based on oxidation of hydroxylamine hydrochloride with selenite ions to nitrous acid, which in turn diazotizes sulfanilic acid which subsequently couples with NEDA to form magenta colored azo dye. The dye thus formed shows a maximum absorbance at 550 nm. The method obeys Beer’s law in the range of 0.05 to 0.26 ppm of Se. Its molar absorptivity, Sandell’s sensitivity, standard deviation and relative standard deviation were found 9.04x10, 8.73x10-3, 0.002, and 0.87% respectively. All the reaction parameters have been optimized. Interferences between the azo reaction and non targeted ions often present in environmental samples were investigated. The method has been successfully applied to the analysis of waste water and plant material. Chemical sensing strips were also been prepared and were successfully applied for detection of selenium in air and semi quantitative determination in water. The advantages of method are its high sensitivity, reproducibility, and the fact that measurement is simple, rapid and low cost. Copyright@ International Science Community Association